Projects per year
Abstract
Succinate dehydrogenase (SDH, complex II), which plays an essential role in mitochondrial respiration and tricarboxylic acid metabolism, requires the assembly of eight nuclear-encoded subunits and the insertion of various cofactors. Here, we report on the characterization of an Arabidopsis thaliana leucine-tyrosine-arginine (LYR) protein family member SDHAF1, (At2g39725) is a factor required for SDH activity. SDHAF1 is located in mitochondria and can fully complement the yeast SDHAF1 deletion strain. Knockdown of SDHAF1 using RNA interference resulted in a decrease in seedling hypocotyl elongation and reduced SDH activity. Proteomic analyses revealed a decreased abundance of various SDH subunits and assembly factors. Protein interaction assays revealed that SDHAF1 can interact exclusively with the Fe-S cluster-containing subunit SDH2 and HSCB, a cochaperone involved in Fe-S cluster complex recruitment. Therefore, we propose that in Arabidopsis, SDHAF1 plays a role in the biogenesis of SDH2 to form the functional complex II, which is essential for mitochondrial respiration and metabolism.
Original language | English |
---|---|
Pages (from-to) | 499-512 |
Number of pages | 14 |
Journal | Plant Journal |
Volume | 110 |
Issue number | 2 |
DOIs | |
Publication status | Published - Apr 2022 |
Fingerprint
Dive into the research topics of 'The mitochondrial LYR protein SDHAF1 is required for succinate dehydrogenase activity in Arabidopsis'. Together they form a unique fingerprint.Projects
- 1 Finished
-
DP210103258 ‘Mitochondrial Biogenesis and Signalling in Plants
Whelan, J. (Investigator 01), Murcha, M. (Investigator 02), Shou, H. (Investigator 03) & Strand, A. (Investigator 04)
ARC Australian Research Council
28/06/21 → 31/12/24
Project: Research