The mitochondrial LYR protein SDHAF1 is required for succinate dehydrogenase activity in Arabidopsis

Ying Li; Katharina Belt; Saad F. Alqahtani; Saurabh Saha; Ricarda Fenske; Olivier Van Aken; James Whelan; A. Harvey Millar; Monika W. Murcha; Shaobai Huang

doi:10.1111/tpj.15684

The mitochondrial LYR protein SDHAF1 is required for succinate dehydrogenase activity in Arabidopsis

Ying Li, Katharina Belt, Saad F. Alqahtani, Saurabh Saha, Ricarda Fenske, Olivier Van Aken, James Whelan, A. Harvey Millar, Monika W. Murcha, Shaobai Huang

Research output: Contribution to journal › Article › peer-review

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Abstract

Succinate dehydrogenase (SDH, complex II), which plays an essential role in mitochondrial respiration and tricarboxylic acid metabolism, requires the assembly of eight nuclear-encoded subunits and the insertion of various cofactors. Here, we report on the characterization of an Arabidopsis thaliana leucine-tyrosine-arginine (LYR) protein family member SDHAF1, (At2g39725) is a factor required for SDH activity. SDHAF1 is located in mitochondria and can fully complement the yeast SDHAF1 deletion strain. Knockdown of SDHAF1 using RNA interference resulted in a decrease in seedling hypocotyl elongation and reduced SDH activity. Proteomic analyses revealed a decreased abundance of various SDH subunits and assembly factors. Protein interaction assays revealed that SDHAF1 can interact exclusively with the Fe-S cluster-containing subunit SDH2 and HSCB, a cochaperone involved in Fe-S cluster complex recruitment. Therefore, we propose that in Arabidopsis, SDHAF1 plays a role in the biogenesis of SDH2 to form the functional complex II, which is essential for mitochondrial respiration and metabolism.

Original language	English
Pages (from-to)	499-512
Number of pages	14
Journal	Plant Journal
Volume	110
Issue number	2
Early online date	26 Feb 2022
DOIs	https://doi.org/10.1111/tpj.15684
Publication status	Published - Apr 2022

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title = "The mitochondrial LYR protein SDHAF1 is required for succinate dehydrogenase activity in Arabidopsis",

abstract = "Succinate dehydrogenase (SDH, complex II), which plays an essential role in mitochondrial respiration and tricarboxylic acid metabolism, requires the assembly of eight nuclear-encoded subunits and the insertion of various cofactors. Here, we report on the characterization of an Arabidopsis thaliana leucine-tyrosine-arginine (LYR) protein family member SDHAF1, (At2g39725) is a factor required for SDH activity. SDHAF1 is located in mitochondria and can fully complement the yeast SDHAF1 deletion strain. Knockdown of SDHAF1 using RNA interference resulted in a decrease in seedling hypocotyl elongation and reduced SDH activity. Proteomic analyses revealed a decreased abundance of various SDH subunits and assembly factors. Protein interaction assays revealed that SDHAF1 can interact exclusively with the Fe-S cluster-containing subunit SDH2 and HSCB, a cochaperone involved in Fe-S cluster complex recruitment. Therefore, we propose that in Arabidopsis, SDHAF1 plays a role in the biogenesis of SDH2 to form the functional complex II, which is essential for mitochondrial respiration and metabolism.",

keywords = "mitochondrial metabolism, complex II, succinate dehydrogenase, assembly factors, iron-sulfur cluster insertion, IRON-SULFUR SUBUNIT, COMPLEX-II, GENE, ACID, PHOTOSYNTHESIS, RESPIRATION, PEROXISOMES, METABOLISM, MEMBRANE, INTERACT",

author = "Ying Li and Katharina Belt and Alqahtani, {Saad F.} and Saurabh Saha and Ricarda Fenske and {Van Aken}, Olivier and James Whelan and Millar, {A. Harvey} and Murcha, {Monika W.} and Shaobai Huang",

year = "2022",

month = apr,

doi = "10.1111/tpj.15684",

language = "English",

volume = "110",

pages = "499--512",

journal = "Plant Journal",

issn = "0960-7412",

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T1 - The mitochondrial LYR protein SDHAF1 is required for succinate dehydrogenase activity in Arabidopsis

AU - Li, Ying

AU - Belt, Katharina

AU - Alqahtani, Saad F.

AU - Saha, Saurabh

AU - Fenske, Ricarda

AU - Van Aken, Olivier

AU - Whelan, James

AU - Millar, A. Harvey

AU - Murcha, Monika W.

AU - Huang, Shaobai

PY - 2022/4

Y1 - 2022/4

N2 - Succinate dehydrogenase (SDH, complex II), which plays an essential role in mitochondrial respiration and tricarboxylic acid metabolism, requires the assembly of eight nuclear-encoded subunits and the insertion of various cofactors. Here, we report on the characterization of an Arabidopsis thaliana leucine-tyrosine-arginine (LYR) protein family member SDHAF1, (At2g39725) is a factor required for SDH activity. SDHAF1 is located in mitochondria and can fully complement the yeast SDHAF1 deletion strain. Knockdown of SDHAF1 using RNA interference resulted in a decrease in seedling hypocotyl elongation and reduced SDH activity. Proteomic analyses revealed a decreased abundance of various SDH subunits and assembly factors. Protein interaction assays revealed that SDHAF1 can interact exclusively with the Fe-S cluster-containing subunit SDH2 and HSCB, a cochaperone involved in Fe-S cluster complex recruitment. Therefore, we propose that in Arabidopsis, SDHAF1 plays a role in the biogenesis of SDH2 to form the functional complex II, which is essential for mitochondrial respiration and metabolism.

AB - Succinate dehydrogenase (SDH, complex II), which plays an essential role in mitochondrial respiration and tricarboxylic acid metabolism, requires the assembly of eight nuclear-encoded subunits and the insertion of various cofactors. Here, we report on the characterization of an Arabidopsis thaliana leucine-tyrosine-arginine (LYR) protein family member SDHAF1, (At2g39725) is a factor required for SDH activity. SDHAF1 is located in mitochondria and can fully complement the yeast SDHAF1 deletion strain. Knockdown of SDHAF1 using RNA interference resulted in a decrease in seedling hypocotyl elongation and reduced SDH activity. Proteomic analyses revealed a decreased abundance of various SDH subunits and assembly factors. Protein interaction assays revealed that SDHAF1 can interact exclusively with the Fe-S cluster-containing subunit SDH2 and HSCB, a cochaperone involved in Fe-S cluster complex recruitment. Therefore, we propose that in Arabidopsis, SDHAF1 plays a role in the biogenesis of SDH2 to form the functional complex II, which is essential for mitochondrial respiration and metabolism.

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KW - COMPLEX-II

KW - GENE

KW - ACID

KW - PHOTOSYNTHESIS

KW - RESPIRATION

KW - PEROXISOMES

KW - METABOLISM

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KW - INTERACT

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JO - Plant Journal

JF - Plant Journal

SN - 0960-7412

IS - 2

ER -

The mitochondrial LYR protein SDHAF1 is required for succinate dehydrogenase activity in Arabidopsis

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