The mitochondrial LYR protein SDHAF1 is required for succinate dehydrogenase activity in Arabidopsis

Ying Li, Katharina Belt, Saad F. Alqahtani, Saurabh Saha, Ricarda Fenske, Olivier Van Aken, James Whelan, A. Harvey Millar, Monika W. Murcha, Shaobai Huang

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)

Abstract

Succinate dehydrogenase (SDH, complex II), which plays an essential role in mitochondrial respiration and tricarboxylic acid metabolism, requires the assembly of eight nuclear-encoded subunits and the insertion of various cofactors. Here, we report on the characterization of an Arabidopsis thaliana leucine-tyrosine-arginine (LYR) protein family member SDHAF1, (At2g39725) is a factor required for SDH activity. SDHAF1 is located in mitochondria and can fully complement the yeast SDHAF1 deletion strain. Knockdown of SDHAF1 using RNA interference resulted in a decrease in seedling hypocotyl elongation and reduced SDH activity. Proteomic analyses revealed a decreased abundance of various SDH subunits and assembly factors. Protein interaction assays revealed that SDHAF1 can interact exclusively with the Fe-S cluster-containing subunit SDH2 and HSCB, a cochaperone involved in Fe-S cluster complex recruitment. Therefore, we propose that in Arabidopsis, SDHAF1 plays a role in the biogenesis of SDH2 to form the functional complex II, which is essential for mitochondrial respiration and metabolism.

Original languageEnglish
Pages (from-to)499-512
Number of pages14
JournalPlant Journal
Volume110
Issue number2
Early online date26 Feb 2022
DOIs
Publication statusPublished - Apr 2022

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