The mitochondrial DNA-associated protein SWIB5 influences mtDNA architecture and homologous recombination

Jonas Blomme, Olivier Van Aken, Jelle Van Leene, Teddy Jégu, Riet De Rycke, Michiel De Bruyne, Jasmien Vercruysse, Jonah Nolf, Twiggy Van Daele, Liesbeth De Milde, Mattias Vermeersch, Catherine Colas des Francs-Small, Geert De Jaeger, Moussa Benhamed, A. Harvey Millar, Dirk Inzé, Nathalie Gonzalez

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7 Citations (Scopus)

Abstract

In addition to the nucleus, mitochondria and chloroplasts in plant cells also contain genomes. Efficient DNA repair pathways are crucial in these organelles to fix damage resulting from endogenous and exogenous factors. Plant organellar genomes are complex compared with their animal counterparts, and although several plant-specific mediators of organelle DNA repair have been reported, many regulators remain to be identified. Here, we show that a mitochondrial SWI/SNF (nucleosome remodeling) complex B protein, SWIB5, is capable of associating with mitochondrial DNA (mtDNA) in Arabidopsis thaliana. Gainand loss-of-function mutants provided evidence for a role of SWIB5 in influencing mtDNA architecture and homologous recombination at specific intermediate-sized repeats both under normal and genotoxic conditions. SWIB5 interacts with other mitochondrial SWIB proteins. Gene expression and mutant phenotypic analysis of SWIB5 and SWIB family members suggests a link between organellar genome maintenance and cell proliferation. Taken together, our work presents a protein family that influences mtDNA architecture and homologous recombination in plants and suggests a link between organelle functioning and plant development.

Original languageEnglish
Pages (from-to)1137-1156
Number of pages20
JournalPlant Cell
Volume29
Issue number5
DOIs
Publication statusPublished - 1 May 2017

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    Blomme, J., Van Aken, O., Van Leene, J., Jégu, T., De Rycke, R., De Bruyne, M., Vercruysse, J., Nolf, J., Van Daele, T., De Milde, L., Vermeersch, M., des Francs-Small, C. C., De Jaeger, G., Benhamed, M., Millar, A. H., Inzé, D., & Gonzalez, N. (2017). The mitochondrial DNA-associated protein SWIB5 influences mtDNA architecture and homologous recombination. Plant Cell, 29(5), 1137-1156. https://doi.org/10.1105/tpc.16.00899