The magnetic susceptibilities of iron deposits in thalassaemic spleen tissue

Seval Hackett, Wanida Chua-Anusorn, P. Pootrakul, Tim St Pierre

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    26 Citations (Scopus)

    Abstract

    The iron-specific magnetic susceptibility of tissue iron deposits is used in the field of non-invasive measurement of tissue iron concentrations. It has generally been assumed to be a constant for all tissue and disease types. The iron-specific magnetic susceptibilities chi(Fe) for spleen tissue samples from 7 transfusion dependent beta-thalassaemia (beta-thal) patients and 11 non-transfusion dependent beta-thalassaemia/Haemoglobin E (beta/E) patients were measured at 37 degrees C. Both groups of patients were iron loaded with no significant difference in the distribution of spleen iron concentrations between the two groups. There was a significant difference between the mean chi(Fe), of the spleen tissue from each group. The non-transfusion dependent beta/E patients had a higher mean (+/- standard deviation) spleen chi(Fe) (1.55 +/- 0.23x10(-6) m(3)/kg Fe). than the transfusion dependent beta-thal patients (1.16 +/- 0.25x10(-6) m(3)/kg Fe). Correlations were observed between chi(Fe) of the spleen tissue and the fraction of magnetic hyperfine split sextet in the Fe-57 Mossbauer spectra of the tissues at 78 K (Spearman rank order correlation r=-0.54, p=0.03) and between chi(Fe) of the spleen tissue and the fraction of doublet in the spectra at 5 K (r=0.58, p=0.02) indicating that chi(Fe) of the spleen tissue is related to the chemical speciation of the iron deposits in the tissue. (c) 2007 Elsevier B.V. All rights reserved.
    Original languageEnglish
    Pages (from-to)330-337
    JournalBiochimica et Biophysica Acta - Molecular Basis of Disease
    Volume1772
    Issue number3
    DOIs
    Publication statusPublished - 2007

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