The investigation of single molecule sequencing to characterise HLA genes for clinical use in allogeneic transplantation

Linh Truong

Research output: ThesisDoctoral Thesis

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Abstract

Experiments presented in this thesis reveal the possibility of producing phase-defined HLA genotypes through the use of multiplexed amplification and nanopore sequencing. We present a breakthrough that provides prospective HLA allele-level typing during solid organ allocation. We encountered challenges throughout the development of HLA nanopore sequencing, specifically in the generation of large datasets. A novel data analysis pipeline was built to utilise cloud resources. When analysing HLA genes on a larger scale, important SNPs and extended haplotypes became more apparent. Finally, future research based on the findings in this thesis should focus on HLA haplotypic associations or MHC block matching.
Original languageEnglish
QualificationDoctor of Philosophy
Awarding Institution
  • The University of Western Australia
Supervisors/Advisors
  • D'Orsogna, Lloyd, Supervisor
  • Martinez, Olga, Supervisor
  • Tilanus, Marcel G.J., Supervisor, External person
  • De Santis, Dianne, Supervisor
  • Groeneweg, Mathijs, Supervisor, External person
Thesis sponsors
Award date9 Nov 2023
DOIs
Publication statusUnpublished - 2022

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