The impact of persistent bacterial bronchitis on the pulmonary microbiome of children

Leah Cuthbertson, Vanessa Craven, Lynne Bingle, William O.C.M. Cookson, Mark L. Everard, Miriam F. Moffatt

    Research output: Contribution to journalArticle

    5 Citations (Scopus)

    Abstract

    Introduction Persistent bacterial bronchitis (PBB) is a leading cause of chronic wet cough in young children. This study aimed to characterise the respiratory bacterial microbiota of healthy children and to assess the impact of the changes associated with the development of PBB. Blind, protected brushings were obtained from 20 healthy controls and 24 children with PBB, with an additional directed sample obtained from PBB patients. DNA was extracted, quantified using a 16S rRNA gene quantitative PCR assay prior to microbial community analysis by 16S rRNA gene sequencing. Results No significant difference in bacterial diversity or community composition (R2 = 0.01, P = 0.36) was observed between paired blind and non-blind brushes, showing that blind brushings are a valid means of accessing the airway microbiota. This has important implications for collecting lower respiratory samples from healthy children. A significant decrease in bacterial diversity (P < 0.001) and change in community composition (R2 = 0.08, P = 0.004) was observed among controls, in comparison with patients. Bacterial communities within patients with PBB were dominated by Proteobacteria, and indicator species analysis showed that Haemophilus and Neisseria were significantly associated with the patient group. In 15 (52.9%) cases the dominant organism by sequencing was not identified by standard routine clinical culture. Conclusion The bacteria present in the lungs of patients with PBB were less diverse in terms of richness and evenness. The results validate the clinical diagnosis, and suggest that more attention to bacterial communities in children with chronic cough may lead to more rapid recognition of this condition with earlier treatment and reduction in disease burden.

    Original languageEnglish
    Article numbere0190075
    JournalPLoS One
    Volume12
    Issue number12
    DOIs
    Publication statusPublished - 1 Dec 2017

    Fingerprint

    bronchitis
    Bronchitis
    Microbiota
    Genes
    lungs
    Lung
    Brushes
    Chemical analysis
    Assays
    Bacteria
    cough
    rRNA Genes
    Cough
    bacterial communities
    DNA
    ribosomal RNA
    Neisseria
    Haemophilus
    Proteobacteria
    burden of disease

    Cite this

    Cuthbertson, L., Craven, V., Bingle, L., Cookson, W. O. C. M., Everard, M. L., & Moffatt, M. F. (2017). The impact of persistent bacterial bronchitis on the pulmonary microbiome of children. PLoS One, 12(12), [e0190075]. https://doi.org/10.1371/journal.pone.0190075
    Cuthbertson, Leah ; Craven, Vanessa ; Bingle, Lynne ; Cookson, William O.C.M. ; Everard, Mark L. ; Moffatt, Miriam F. / The impact of persistent bacterial bronchitis on the pulmonary microbiome of children. In: PLoS One. 2017 ; Vol. 12, No. 12.
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    abstract = "Introduction Persistent bacterial bronchitis (PBB) is a leading cause of chronic wet cough in young children. This study aimed to characterise the respiratory bacterial microbiota of healthy children and to assess the impact of the changes associated with the development of PBB. Blind, protected brushings were obtained from 20 healthy controls and 24 children with PBB, with an additional directed sample obtained from PBB patients. DNA was extracted, quantified using a 16S rRNA gene quantitative PCR assay prior to microbial community analysis by 16S rRNA gene sequencing. Results No significant difference in bacterial diversity or community composition (R2 = 0.01, P = 0.36) was observed between paired blind and non-blind brushes, showing that blind brushings are a valid means of accessing the airway microbiota. This has important implications for collecting lower respiratory samples from healthy children. A significant decrease in bacterial diversity (P < 0.001) and change in community composition (R2 = 0.08, P = 0.004) was observed among controls, in comparison with patients. Bacterial communities within patients with PBB were dominated by Proteobacteria, and indicator species analysis showed that Haemophilus and Neisseria were significantly associated with the patient group. In 15 (52.9{\%}) cases the dominant organism by sequencing was not identified by standard routine clinical culture. Conclusion The bacteria present in the lungs of patients with PBB were less diverse in terms of richness and evenness. The results validate the clinical diagnosis, and suggest that more attention to bacterial communities in children with chronic cough may lead to more rapid recognition of this condition with earlier treatment and reduction in disease burden.",
    author = "Leah Cuthbertson and Vanessa Craven and Lynne Bingle and Cookson, {William O.C.M.} and Everard, {Mark L.} and Moffatt, {Miriam F.}",
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    Cuthbertson, L, Craven, V, Bingle, L, Cookson, WOCM, Everard, ML & Moffatt, MF 2017, 'The impact of persistent bacterial bronchitis on the pulmonary microbiome of children' PLoS One, vol. 12, no. 12, e0190075. https://doi.org/10.1371/journal.pone.0190075

    The impact of persistent bacterial bronchitis on the pulmonary microbiome of children. / Cuthbertson, Leah; Craven, Vanessa; Bingle, Lynne; Cookson, William O.C.M.; Everard, Mark L.; Moffatt, Miriam F.

    In: PLoS One, Vol. 12, No. 12, e0190075, 01.12.2017.

    Research output: Contribution to journalArticle

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    AU - Craven, Vanessa

    AU - Bingle, Lynne

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    AU - Everard, Mark L.

    AU - Moffatt, Miriam F.

    PY - 2017/12/1

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    N2 - Introduction Persistent bacterial bronchitis (PBB) is a leading cause of chronic wet cough in young children. This study aimed to characterise the respiratory bacterial microbiota of healthy children and to assess the impact of the changes associated with the development of PBB. Blind, protected brushings were obtained from 20 healthy controls and 24 children with PBB, with an additional directed sample obtained from PBB patients. DNA was extracted, quantified using a 16S rRNA gene quantitative PCR assay prior to microbial community analysis by 16S rRNA gene sequencing. Results No significant difference in bacterial diversity or community composition (R2 = 0.01, P = 0.36) was observed between paired blind and non-blind brushes, showing that blind brushings are a valid means of accessing the airway microbiota. This has important implications for collecting lower respiratory samples from healthy children. A significant decrease in bacterial diversity (P < 0.001) and change in community composition (R2 = 0.08, P = 0.004) was observed among controls, in comparison with patients. Bacterial communities within patients with PBB were dominated by Proteobacteria, and indicator species analysis showed that Haemophilus and Neisseria were significantly associated with the patient group. In 15 (52.9%) cases the dominant organism by sequencing was not identified by standard routine clinical culture. Conclusion The bacteria present in the lungs of patients with PBB were less diverse in terms of richness and evenness. The results validate the clinical diagnosis, and suggest that more attention to bacterial communities in children with chronic cough may lead to more rapid recognition of this condition with earlier treatment and reduction in disease burden.

    AB - Introduction Persistent bacterial bronchitis (PBB) is a leading cause of chronic wet cough in young children. This study aimed to characterise the respiratory bacterial microbiota of healthy children and to assess the impact of the changes associated with the development of PBB. Blind, protected brushings were obtained from 20 healthy controls and 24 children with PBB, with an additional directed sample obtained from PBB patients. DNA was extracted, quantified using a 16S rRNA gene quantitative PCR assay prior to microbial community analysis by 16S rRNA gene sequencing. Results No significant difference in bacterial diversity or community composition (R2 = 0.01, P = 0.36) was observed between paired blind and non-blind brushes, showing that blind brushings are a valid means of accessing the airway microbiota. This has important implications for collecting lower respiratory samples from healthy children. A significant decrease in bacterial diversity (P < 0.001) and change in community composition (R2 = 0.08, P = 0.004) was observed among controls, in comparison with patients. Bacterial communities within patients with PBB were dominated by Proteobacteria, and indicator species analysis showed that Haemophilus and Neisseria were significantly associated with the patient group. In 15 (52.9%) cases the dominant organism by sequencing was not identified by standard routine clinical culture. Conclusion The bacteria present in the lungs of patients with PBB were less diverse in terms of richness and evenness. The results validate the clinical diagnosis, and suggest that more attention to bacterial communities in children with chronic cough may lead to more rapid recognition of this condition with earlier treatment and reduction in disease burden.

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