The impact of chronic intrauterine inflammation on the physiologic and neurodevelopmental consequences of intermittent umbilical cord occlusion in fetal sheep

Ilias Nitsos, John Newnham, S.M. Rees, R.K. Harding, T.J.M. Moss

    Research output: Contribution to journalArticle

    7 Citations (Scopus)

    Abstract

    Objective: To determine the effect of intrauterine inflammation on fetal responses to umbilical cord occlusion (UCO).

    Study Design: In pregnant sheep, lipopolysaccharide (LPS) or saline (SAL) was infused intra-amniotically for 4 weeks from 80 days of gestation (d). At 110 d, fetuses were instrumented for UCOs (5 2-minutes, 30-minute intervals: LPS + UCO, n6; SAL + UCO, n8) or no UCO (sham, n6) on 117 and 118 d. Tissues were collected at 126 d.

    Results: Fetal physiological responses to UCO were similar between LPS + UCO and SAL + UCO. Histologic chorioamnionitis and increased amniotic fluid interleukin 8 (IL-8) were observed in LPS + UCO pregnancies (versus SAL + UCO, P <.05). CNPase-positive oligodendrocyte number in the cerebral white matter was lower in LPS + UCO and SAL + UCO than sham (P <.05); there was no effect on astrocytes or activated microglia/macrophages. Two of the SAL + UCO fetuses had white matter lesions; none were observed in LPS + UCO or sham.

    Conclusion: Chronic pre-existing intrauterine inflammation did not exacerbate fetal brain injury induced by intermittent UCO.

    Original languageEnglish
    Pages (from-to)658-670
    JournalReproductive Sciences
    Volume21
    Issue number5
    Early online date18 Mar 2011
    DOIs
    Publication statusPublished - May 2014

      Fingerprint

    Cite this