The impact of chronic intrauterine inflammation on the physiologic and neurodevelopmental consequences of intermittent umbilical cord occlusion in fetal sheep

Ilias Nitsos; John Newnham; S.M. Rees; R.K. Harding; T.J.M. Moss

doi:10.1177/1933719111399928

The impact of chronic intrauterine inflammation on the physiologic and neurodevelopmental consequences of intermittent umbilical cord occlusion in fetal sheep

Ilias Nitsos, John Newnham, S.M. Rees, R.K. Harding, T.J.M. Moss

Research output: Contribution to journal › Article › peer-review

9 Citations (Scopus)

Abstract

Objective: To determine the effect of intrauterine inflammation on fetal responses to umbilical cord occlusion (UCO).

Study Design: In pregnant sheep, lipopolysaccharide (LPS) or saline (SAL) was infused intra-amniotically for 4 weeks from 80 days of gestation (d). At 110 d, fetuses were instrumented for UCOs (5 2-minutes, 30-minute intervals: LPS + UCO, n6; SAL + UCO, n8) or no UCO (sham, n6) on 117 and 118 d. Tissues were collected at 126 d.

Results: Fetal physiological responses to UCO were similar between LPS + UCO and SAL + UCO. Histologic chorioamnionitis and increased amniotic fluid interleukin 8 (IL-8) were observed in LPS + UCO pregnancies (versus SAL + UCO, P <.05). CNPase-positive oligodendrocyte number in the cerebral white matter was lower in LPS + UCO and SAL + UCO than sham (P <.05); there was no effect on astrocytes or activated microglia/macrophages. Two of the SAL + UCO fetuses had white matter lesions; none were observed in LPS + UCO or sham.

Conclusion: Chronic pre-existing intrauterine inflammation did not exacerbate fetal brain injury induced by intermittent UCO.

Original language	English
Pages (from-to)	658-670
Journal	Reproductive Sciences
Volume	21
Issue number	5
Early online date	18 Mar 2011
DOIs	https://doi.org/10.1177/1933719111399928
Publication status	Published - May 2014

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10.1177/1933719111399928

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3984479/

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title = "The impact of chronic intrauterine inflammation on the physiologic and neurodevelopmental consequences of intermittent umbilical cord occlusion in fetal sheep",

abstract = "Objective: To determine the effect of intrauterine inflammation on fetal responses to umbilical cord occlusion (UCO). Study Design: In pregnant sheep, lipopolysaccharide (LPS) or saline (SAL) was infused intra-amniotically for 4 weeks from 80 days of gestation (d). At 110 d, fetuses were instrumented for UCOs (5 2-minutes, 30-minute intervals: LPS + UCO, n6; SAL + UCO, n8) or no UCO (sham, n6) on 117 and 118 d. Tissues were collected at 126 d. Results: Fetal physiological responses to UCO were similar between LPS + UCO and SAL + UCO. Histologic chorioamnionitis and increased amniotic fluid interleukin 8 (IL-8) were observed in LPS + UCO pregnancies (versus SAL + UCO, P <.05). CNPase-positive oligodendrocyte number in the cerebral white matter was lower in LPS + UCO and SAL + UCO than sham (P <.05); there was no effect on astrocytes or activated microglia/macrophages. Two of the SAL + UCO fetuses had white matter lesions; none were observed in LPS + UCO or sham. Conclusion: Chronic pre-existing intrauterine inflammation did not exacerbate fetal brain injury induced by intermittent UCO.",

author = "Ilias Nitsos and John Newnham and S.M. Rees and R.K. Harding and T.J.M. Moss",

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AU - Harding, R.K.

AU - Moss, T.J.M.

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N2 - Objective: To determine the effect of intrauterine inflammation on fetal responses to umbilical cord occlusion (UCO). Study Design: In pregnant sheep, lipopolysaccharide (LPS) or saline (SAL) was infused intra-amniotically for 4 weeks from 80 days of gestation (d). At 110 d, fetuses were instrumented for UCOs (5 2-minutes, 30-minute intervals: LPS + UCO, n6; SAL + UCO, n8) or no UCO (sham, n6) on 117 and 118 d. Tissues were collected at 126 d. Results: Fetal physiological responses to UCO were similar between LPS + UCO and SAL + UCO. Histologic chorioamnionitis and increased amniotic fluid interleukin 8 (IL-8) were observed in LPS + UCO pregnancies (versus SAL + UCO, P <.05). CNPase-positive oligodendrocyte number in the cerebral white matter was lower in LPS + UCO and SAL + UCO than sham (P <.05); there was no effect on astrocytes or activated microglia/macrophages. Two of the SAL + UCO fetuses had white matter lesions; none were observed in LPS + UCO or sham. Conclusion: Chronic pre-existing intrauterine inflammation did not exacerbate fetal brain injury induced by intermittent UCO.

AB - Objective: To determine the effect of intrauterine inflammation on fetal responses to umbilical cord occlusion (UCO). Study Design: In pregnant sheep, lipopolysaccharide (LPS) or saline (SAL) was infused intra-amniotically for 4 weeks from 80 days of gestation (d). At 110 d, fetuses were instrumented for UCOs (5 2-minutes, 30-minute intervals: LPS + UCO, n6; SAL + UCO, n8) or no UCO (sham, n6) on 117 and 118 d. Tissues were collected at 126 d. Results: Fetal physiological responses to UCO were similar between LPS + UCO and SAL + UCO. Histologic chorioamnionitis and increased amniotic fluid interleukin 8 (IL-8) were observed in LPS + UCO pregnancies (versus SAL + UCO, P <.05). CNPase-positive oligodendrocyte number in the cerebral white matter was lower in LPS + UCO and SAL + UCO than sham (P <.05); there was no effect on astrocytes or activated microglia/macrophages. Two of the SAL + UCO fetuses had white matter lesions; none were observed in LPS + UCO or sham. Conclusion: Chronic pre-existing intrauterine inflammation did not exacerbate fetal brain injury induced by intermittent UCO.

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The impact of chronic intrauterine inflammation on the physiologic and neurodevelopmental consequences of intermittent umbilical cord occlusion in fetal sheep

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