The impact of chronic intrauterine inflammation on the physiologic and neurodevelopmental consequences of intermittent umbilical cord occlusion in fetal sheep

Ilias Nitsos, John Newnham, S.M. Rees, R.K. Harding, T.J.M. Moss

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    Objective: To determine the effect of intrauterine inflammation on fetal responses to umbilical cord occlusion (UCO).

    Study Design: In pregnant sheep, lipopolysaccharide (LPS) or saline (SAL) was infused intra-amniotically for 4 weeks from 80 days of gestation (d). At 110 d, fetuses were instrumented for UCOs (5 2-minutes, 30-minute intervals: LPS + UCO, n6; SAL + UCO, n8) or no UCO (sham, n6) on 117 and 118 d. Tissues were collected at 126 d.

    Results: Fetal physiological responses to UCO were similar between LPS + UCO and SAL + UCO. Histologic chorioamnionitis and increased amniotic fluid interleukin 8 (IL-8) were observed in LPS + UCO pregnancies (versus SAL + UCO, P <.05). CNPase-positive oligodendrocyte number in the cerebral white matter was lower in LPS + UCO and SAL + UCO than sham (P <.05); there was no effect on astrocytes or activated microglia/macrophages. Two of the SAL + UCO fetuses had white matter lesions; none were observed in LPS + UCO or sham.

    Conclusion: Chronic pre-existing intrauterine inflammation did not exacerbate fetal brain injury induced by intermittent UCO.

    Original languageEnglish
    Pages (from-to)658-670
    JournalReproductive Sciences
    Issue number5
    Early online date18 Mar 2011
    Publication statusPublished - May 2014


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