The fumagillin biosynthetic gene cluster in Aspergillus fumigatus encodes a cryptic terpene cyclase involved in the formation of β-trans-bergamotene

H.C. Lin, Heng Chooi, S. Dhingra, W. Xu, A.M. Calvo, Y. Tang

Research output: Contribution to journalArticle

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Abstract

Fumagillin 1 is a meroterpenoid from Aspergillus fumigatus that is known for its anti-angiogenic activity by binding to human methionine aminopeptidase 2. The genetic and molecular basis for biosynthesis of 1 had been an enigma despite the availability of the A. fumigatus genome sequence. Here, we report the identification and verification of the fma gene cluster, followed by characterization of the polyketide synthase and acyltransferase involved in biosynthesis of the dioic acid portion of 1. More significantly, we uncovered the elusive β-trans-bergamotene synthase in A. fumigatus as a membrane-bound terpene cyclase. © 2013 American Chemical Society.
Original languageEnglish
Pages (from-to)4616-4619
JournalJournal of the American Chemical Society
Volume135
Issue number12
DOIs
Publication statusPublished - 2013

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Terpenes
Aspergillus fumigatus
Aspergillus
Biosynthesis
Multigene Family
Genes
Polyketide Synthases
Acyltransferases
Availability
Membranes
Acids
Molecular Biology
Genome
fumagillin
methionine aminopeptidase 2

Cite this

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title = "The fumagillin biosynthetic gene cluster in Aspergillus fumigatus encodes a cryptic terpene cyclase involved in the formation of β-trans-bergamotene",
abstract = "Fumagillin 1 is a meroterpenoid from Aspergillus fumigatus that is known for its anti-angiogenic activity by binding to human methionine aminopeptidase 2. The genetic and molecular basis for biosynthesis of 1 had been an enigma despite the availability of the A. fumigatus genome sequence. Here, we report the identification and verification of the fma gene cluster, followed by characterization of the polyketide synthase and acyltransferase involved in biosynthesis of the dioic acid portion of 1. More significantly, we uncovered the elusive β-trans-bergamotene synthase in A. fumigatus as a membrane-bound terpene cyclase. {\circledC} 2013 American Chemical Society.",
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The fumagillin biosynthetic gene cluster in Aspergillus fumigatus encodes a cryptic terpene cyclase involved in the formation of β-trans-bergamotene. / Lin, H.C.; Chooi, Heng; Dhingra, S.; Xu, W.; Calvo, A.M.; Tang, Y.

In: Journal of the American Chemical Society, Vol. 135, No. 12, 2013, p. 4616-4619.

Research output: Contribution to journalArticle

TY - JOUR

T1 - The fumagillin biosynthetic gene cluster in Aspergillus fumigatus encodes a cryptic terpene cyclase involved in the formation of β-trans-bergamotene

AU - Lin, H.C.

AU - Chooi, Heng

AU - Dhingra, S.

AU - Xu, W.

AU - Calvo, A.M.

AU - Tang, Y.

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AB - Fumagillin 1 is a meroterpenoid from Aspergillus fumigatus that is known for its anti-angiogenic activity by binding to human methionine aminopeptidase 2. The genetic and molecular basis for biosynthesis of 1 had been an enigma despite the availability of the A. fumigatus genome sequence. Here, we report the identification and verification of the fma gene cluster, followed by characterization of the polyketide synthase and acyltransferase involved in biosynthesis of the dioic acid portion of 1. More significantly, we uncovered the elusive β-trans-bergamotene synthase in A. fumigatus as a membrane-bound terpene cyclase. © 2013 American Chemical Society.

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