The expression of apoptosis related genes in the first trimester human placenta using a short term in vitro model

Adrian Charles; S. Hisheh; D. Liu; R.M. Rao; Brendan Waddell; Jan Dickinson; A.J. Rao; A.M. Dharmarajan

doi:10.1007/s10495-005-6068-5

The expression of apoptosis related genes in the first trimester human placenta using a short term in vitro model

Adrian Charles, S. Hisheh, D. Liu, R.M. Rao, Brendan Waddell, Jan Dickinson, A.J. Rao, A.M. Dharmarajan

Graduate Research School

Research output: Contribution to journal › Article › peer-review

8 Citations (Scopus)

Abstract

Using in vitro model for studying the induction and inhibition of spontaneous apoptosis in human first trimester placental villi, mediated by the free radical scavenger SOD, we have examined the expression of bcl-xL, bax, Caspase-3 and PARP (Poly ADP-ribosyl). An increase in apoptosis was associated with activation of PARP and an increase and activation of Caspase-3. There was no significant change in bcl-x or bax. Therefore bcl-x and bax do not appear to have a significant role in apoptosis in the first trimester in vitro. Cleavage of Caspase-3 rather than transcriptional regulation appears to be the main determinant of Caspase-3 activity in first trimester placental villi.

Original language	English
Pages (from-to)	135-140
Journal	Apoptosis
Volume	10
Issue number	1
DOIs	https://doi.org/10.1007/s10495-005-6068-5
Publication status	Published - 2005

Access to Document

10.1007/s10495-005-6068-5

Cite this

@article{f92fc8adaddf42bcb25ad0038d96204b,

title = "The expression of apoptosis related genes in the first trimester human placenta using a short term in vitro model",

abstract = "Using in vitro model for studying the induction and inhibition of spontaneous apoptosis in human first trimester placental villi, mediated by the free radical scavenger SOD, we have examined the expression of bcl-xL, bax, Caspase-3 and PARP (Poly ADP-ribosyl). An increase in apoptosis was associated with activation of PARP and an increase and activation of Caspase-3. There was no significant change in bcl-x or bax. Therefore bcl-x and bax do not appear to have a significant role in apoptosis in the first trimester in vitro. Cleavage of Caspase-3 rather than transcriptional regulation appears to be the main determinant of Caspase-3 activity in first trimester placental villi.",

author = "Adrian Charles and S. Hisheh and D. Liu and R.M. Rao and Brendan Waddell and Jan Dickinson and A.J. Rao and A.M. Dharmarajan",

year = "2005",

doi = "10.1007/s10495-005-6068-5",

language = "English",

volume = "10",

pages = "135--140",

journal = "Apoptosis",

issn = "1360-8185",

publisher = "Springer",

number = "1",

}

TY - JOUR

T1 - The expression of apoptosis related genes in the first trimester human placenta using a short term in vitro model

AU - Charles, Adrian

AU - Hisheh, S.

AU - Liu, D.

AU - Rao, R.M.

AU - Waddell, Brendan

AU - Dickinson, Jan

AU - Rao, A.J.

AU - Dharmarajan, A.M.

PY - 2005

Y1 - 2005

N2 - Using in vitro model for studying the induction and inhibition of spontaneous apoptosis in human first trimester placental villi, mediated by the free radical scavenger SOD, we have examined the expression of bcl-xL, bax, Caspase-3 and PARP (Poly ADP-ribosyl). An increase in apoptosis was associated with activation of PARP and an increase and activation of Caspase-3. There was no significant change in bcl-x or bax. Therefore bcl-x and bax do not appear to have a significant role in apoptosis in the first trimester in vitro. Cleavage of Caspase-3 rather than transcriptional regulation appears to be the main determinant of Caspase-3 activity in first trimester placental villi.

AB - Using in vitro model for studying the induction and inhibition of spontaneous apoptosis in human first trimester placental villi, mediated by the free radical scavenger SOD, we have examined the expression of bcl-xL, bax, Caspase-3 and PARP (Poly ADP-ribosyl). An increase in apoptosis was associated with activation of PARP and an increase and activation of Caspase-3. There was no significant change in bcl-x or bax. Therefore bcl-x and bax do not appear to have a significant role in apoptosis in the first trimester in vitro. Cleavage of Caspase-3 rather than transcriptional regulation appears to be the main determinant of Caspase-3 activity in first trimester placental villi.

U2 - 10.1007/s10495-005-6068-5

DO - 10.1007/s10495-005-6068-5

M3 - Article

C2 - 15711929

SN - 1360-8185

VL - 10

SP - 135

EP - 140

JO - Apoptosis

JF - Apoptosis

IS - 1

ER -

The expression of apoptosis related genes in the first trimester human placenta using a short term in vitro model

Abstract

Access to Document

Fingerprint

Cite this