The expanding CRISPR toolbox for natural product discovery and engineering in filamentous fungi

Clara Woodcraft; Yit-Heng Chooi; Indra Roux

doi:10.1039/d2np00055e

The expanding CRISPR toolbox for natural product discovery and engineering in filamentous fungi

Clara Woodcraft, Yit-Heng Chooi, Indra Roux

Research output: Contribution to journal › Review article › peer-review

11 Citations (Scopus)

Abstract

Covering: up to May 2022 Fungal genetics has transformed natural product research by enabling the elucidation of cryptic metabolites and biosynthetic steps. The enhanced capability to add, subtract, modulate, and rewrite genes via CRISPR/Cas technologies has opened up avenues for the manipulation of biosynthetic gene clusters across diverse filamentous fungi. This review discusses the innovative and diverse strategies for fungal natural product discovery and engineering made possible by CRISPR/Cas-based tools. We also provide a guide into multiple angles of CRISPR/Cas experiment design, and discuss current gaps in genetic tool development for filamentous fungi and the promising opportunities for natural product research.

Original language	English
Pages (from-to)	158-173
Number of pages	16
Journal	Natural Product Reports
Volume	40
Issue number	1
DOIs	https://doi.org/10.1039/d2np00055e
Publication status	Published - 7 Oct 2022

Access to Document

10.1039/d2np00055e

Cite this

@article{30ca9ac926554ff591407cfb70f966cd,

title = "The expanding CRISPR toolbox for natural product discovery and engineering in filamentous fungi",

abstract = "Covering: up to May 2022 Fungal genetics has transformed natural product research by enabling the elucidation of cryptic metabolites and biosynthetic steps. The enhanced capability to add, subtract, modulate, and rewrite genes via CRISPR/Cas technologies has opened up avenues for the manipulation of biosynthetic gene clusters across diverse filamentous fungi. This review discusses the innovative and diverse strategies for fungal natural product discovery and engineering made possible by CRISPR/Cas-based tools. We also provide a guide into multiple angles of CRISPR/Cas experiment design, and discuss current gaps in genetic tool development for filamentous fungi and the promising opportunities for natural product research.",

keywords = "BIOSYNTHETIC GENE CLUSTERS, GENOMIC DNA, SHIRAIA-BAMBUSICOLA, BASE, RNA, IDENTIFICATION, SYSTEM, ACID, RECONSTITUTION, ENDONUCLEASE",

author = "Clara Woodcraft and Yit-Heng Chooi and Indra Roux",

year = "2022",

month = oct,

day = "7",

doi = "10.1039/d2np00055e",

language = "English",

volume = "40",

pages = "158--173",

journal = "Natural Product Reports",

issn = "0265-0568",

publisher = "ROYAL SOC CHEMISTRY",

number = "1",

}

TY - JOUR

T1 - The expanding CRISPR toolbox for natural product discovery and engineering in filamentous fungi

AU - Woodcraft, Clara

AU - Chooi, Yit-Heng

AU - Roux, Indra

PY - 2022/10/7

Y1 - 2022/10/7

N2 - Covering: up to May 2022 Fungal genetics has transformed natural product research by enabling the elucidation of cryptic metabolites and biosynthetic steps. The enhanced capability to add, subtract, modulate, and rewrite genes via CRISPR/Cas technologies has opened up avenues for the manipulation of biosynthetic gene clusters across diverse filamentous fungi. This review discusses the innovative and diverse strategies for fungal natural product discovery and engineering made possible by CRISPR/Cas-based tools. We also provide a guide into multiple angles of CRISPR/Cas experiment design, and discuss current gaps in genetic tool development for filamentous fungi and the promising opportunities for natural product research.

AB - Covering: up to May 2022 Fungal genetics has transformed natural product research by enabling the elucidation of cryptic metabolites and biosynthetic steps. The enhanced capability to add, subtract, modulate, and rewrite genes via CRISPR/Cas technologies has opened up avenues for the manipulation of biosynthetic gene clusters across diverse filamentous fungi. This review discusses the innovative and diverse strategies for fungal natural product discovery and engineering made possible by CRISPR/Cas-based tools. We also provide a guide into multiple angles of CRISPR/Cas experiment design, and discuss current gaps in genetic tool development for filamentous fungi and the promising opportunities for natural product research.

KW - BIOSYNTHETIC GENE CLUSTERS

KW - GENOMIC DNA

KW - SHIRAIA-BAMBUSICOLA

KW - BASE

KW - RNA

KW - IDENTIFICATION

KW - SYSTEM

KW - ACID

KW - RECONSTITUTION

KW - ENDONUCLEASE

UR - http://www.scopus.com/inward/record.url?scp=85140914376&partnerID=8YFLogxK

UR - https://www.webofscience.com/wos/woscc/full-record/WOS:000864806900001

U2 - 10.1039/d2np00055e

DO - 10.1039/d2np00055e

M3 - Review article

C2 - 36205232

SN - 0265-0568

VL - 40

SP - 158

EP - 173

JO - Natural Product Reports

JF - Natural Product Reports

IS - 1

ER -

The expanding CRISPR toolbox for natural product discovery and engineering in filamentous fungi

Abstract

Access to Document

Other files and links

Fingerprint

CRISPR-based pathway activation for bioactive molecule discovery in fungi

Uncovering the Hidden Secondary Metabolite Biosynthetic Potential of Fungi

Cite this

The expanding CRISPR toolbox for natural product discovery and engineering in filamentous fungi

Abstract

Access to Document

Other files and links

Fingerprint

Projects

CRISPR-based pathway activation for bioactive molecule discovery in fungi

Uncovering the Hidden Secondary Metabolite Biosynthetic Potential of Fungi

Cite this