The effect of ovarian reserve and receptor signalling on granulosa cell apoptosis during human follicle development

Sheena L.P. Regan; Phil G. Knight; John L. Yovich; James D. Stanger; Yee Leung; Frank Arfuso; Ghanim Almahbobi; Arun Dharmarajan

doi:10.1016/j.mce.2017.11.002

The effect of ovarian reserve and receptor signalling on granulosa cell apoptosis during human follicle development

Sheena L.P. Regan, Phil G. Knight, John L. Yovich, James D. Stanger, Yee Leung, Frank Arfuso, Ghanim Almahbobi, Arun Dharmarajan

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41 Citations (Scopus)

Abstract

The poor oocyte quality in older women has previously been linked to the depletion of the ovarian reserve of primordial follicles and an increase in granulosal apoptosis. Granulosa cells were collected from 198 follicles and individually analysed by flow cytometry. In the young IVF patients, the level of apoptosis was inversely proportional to the expression of bone morphogenetic protein (BMPR1B) and follicle stimulating hormone (FSH) receptors. Conversely, in the older patients this relationship became dysregulated. In the older patients, at the time of preovulatory maturation, the reduced apoptosis reflects the poor mitogenic growth turnover rate of healthy follicles rather than the death rate in an atretic follicle. Restoring an optimum receptor density and down-regulation of receptors may improve oocyte quality and the pregnancy rate in older women.

Original language	English
Pages (from-to)	219-227
Number of pages	9
Journal	Molecular and Cellular Endocrinology
Volume	470
DOIs	https://doi.org/10.1016/j.mce.2017.11.002
Publication status	Published - 15 Jul 2018
Externally published	Yes

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title = "The effect of ovarian reserve and receptor signalling on granulosa cell apoptosis during human follicle development",

abstract = "The poor oocyte quality in older women has previously been linked to the depletion of the ovarian reserve of primordial follicles and an increase in granulosal apoptosis. Granulosa cells were collected from 198 follicles and individually analysed by flow cytometry. In the young IVF patients, the level of apoptosis was inversely proportional to the expression of bone morphogenetic protein (BMPR1B) and follicle stimulating hormone (FSH) receptors. Conversely, in the older patients this relationship became dysregulated. In the older patients, at the time of preovulatory maturation, the reduced apoptosis reflects the poor mitogenic growth turnover rate of healthy follicles rather than the death rate in an atretic follicle. Restoring an optimum receptor density and down-regulation of receptors may improve oocyte quality and the pregnancy rate in older women.",

keywords = "Ageing, Apoptosis, Fertility, Granulosa cell, Receptor expression",

author = "Regan, {Sheena L.P.} and Knight, {Phil G.} and Yovich, {John L.} and Stanger, {James D.} and Yee Leung and Frank Arfuso and Ghanim Almahbobi and Arun Dharmarajan",

note = "Funding Information: S.L.P.R. was a recipient of an Australian Postgraduate Award and a Curtin University Postgraduate Scholarship. This work was supported by additional private external funding, which was gratefully accepted from Denby Macgregor. Publisher Copyright: {\textcopyright} 2017 Elsevier B.V.",

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doi = "10.1016/j.mce.2017.11.002",

language = "English",

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AU - Regan, Sheena L.P.

AU - Knight, Phil G.

AU - Yovich, John L.

AU - Stanger, James D.

AU - Leung, Yee

AU - Arfuso, Frank

AU - Almahbobi, Ghanim

AU - Dharmarajan, Arun

N1 - Funding Information: S.L.P.R. was a recipient of an Australian Postgraduate Award and a Curtin University Postgraduate Scholarship. This work was supported by additional private external funding, which was gratefully accepted from Denby Macgregor. Publisher Copyright: © 2017 Elsevier B.V.

PY - 2018/7/15

Y1 - 2018/7/15

N2 - The poor oocyte quality in older women has previously been linked to the depletion of the ovarian reserve of primordial follicles and an increase in granulosal apoptosis. Granulosa cells were collected from 198 follicles and individually analysed by flow cytometry. In the young IVF patients, the level of apoptosis was inversely proportional to the expression of bone morphogenetic protein (BMPR1B) and follicle stimulating hormone (FSH) receptors. Conversely, in the older patients this relationship became dysregulated. In the older patients, at the time of preovulatory maturation, the reduced apoptosis reflects the poor mitogenic growth turnover rate of healthy follicles rather than the death rate in an atretic follicle. Restoring an optimum receptor density and down-regulation of receptors may improve oocyte quality and the pregnancy rate in older women.

AB - The poor oocyte quality in older women has previously been linked to the depletion of the ovarian reserve of primordial follicles and an increase in granulosal apoptosis. Granulosa cells were collected from 198 follicles and individually analysed by flow cytometry. In the young IVF patients, the level of apoptosis was inversely proportional to the expression of bone morphogenetic protein (BMPR1B) and follicle stimulating hormone (FSH) receptors. Conversely, in the older patients this relationship became dysregulated. In the older patients, at the time of preovulatory maturation, the reduced apoptosis reflects the poor mitogenic growth turnover rate of healthy follicles rather than the death rate in an atretic follicle. Restoring an optimum receptor density and down-regulation of receptors may improve oocyte quality and the pregnancy rate in older women.

KW - Ageing

KW - Apoptosis

KW - Fertility

KW - Granulosa cell

KW - Receptor expression

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DO - 10.1016/j.mce.2017.11.002

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AN - SCOPUS:85035136523

SN - 0303-7207

VL - 470

SP - 219

EP - 227

JO - Molecular and Cellular Endocrinology

JF - Molecular and Cellular Endocrinology

ER -

The effect of ovarian reserve and receptor signalling on granulosa cell apoptosis during human follicle development

Abstract

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