The effect of myelotomy following low thoracic spinal cord compression injury in rats

Carolin Meyer; Habib Bendella; Svenja Rink; Robin Gensch; Robert Seitz; Gregor Stein; Marilena Manthou; Theodora Papamitsou; Makoto Nakamura; Bertil Bouillon; Mary Galea; Peter Batchelor; Sarah Dunlop; Doychin Angelov

doi:10.1016/j.expneurol.2018.04.011

The effect of myelotomy following low thoracic spinal cord compression injury in rats

Carolin Meyer, Habib Bendella, Svenja Rink, Robin Gensch, Robert Seitz, Gregor Stein, Marilena Manthou, Theodora Papamitsou, Makoto Nakamura, Bertil Bouillon, Mary Galea, Peter Batchelor, Sarah Dunlop, Doychin Angelov

School of Biological Sciences

Research output: Contribution to journal › Article › peer-review

10 Citations (Scopus)

Abstract

Myelotomy is a surgical procedure allowing removal of extravasated blood and necrotic tissue that is thought to attenuate secondary injury as well as promote recovery in experimental spinal cord injury (SCI) models and humans. Here we examined in rat whether myelotomy at 48 h after low-thoracic compressive SCI provided any benefit over a 12 week period. Compared to animals receiving SCI alone, myelotomy worsened BBB scores (p < 0.05) and also did not improve plantar stepping, ladder climbing, urinary bladder voiding or sensory function (thermal latency) during the 12-week period. Quantitative analyses of tissue sections at 12 weeks showed that myelotomy also did not reduce lesion volume nor alter immunohistochemical markers of axons in spared white matter bridges, microglia, astrocytes or serotinergic fibres. However, myelotomy reduced synaptophysin expression, a marker of synaptic plasticity. We conclude that further studies are required to evaluate myelotomy after SCI. (142 words).

Original language	English
Pages (from-to)	10-21
Number of pages	12
Journal	Experimental Neurology
Volume	306
DOIs	https://doi.org/10.1016/j.expneurol.2018.04.011
Publication status	Published - 1 Aug 2018

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title = "The effect of myelotomy following low thoracic spinal cord compression injury in rats",

abstract = "Myelotomy is a surgical procedure allowing removal of extravasated blood and necrotic tissue that is thought to attenuate secondary injury as well as promote recovery in experimental spinal cord injury (SCI) models and humans. Here we examined in rat whether myelotomy at 48 h after low-thoracic compressive SCI provided any benefit over a 12 week period. Compared to animals receiving SCI alone, myelotomy worsened BBB scores (p < 0.05) and also did not improve plantar stepping, ladder climbing, urinary bladder voiding or sensory function (thermal latency) during the 12-week period. Quantitative analyses of tissue sections at 12 weeks showed that myelotomy also did not reduce lesion volume nor alter immunohistochemical markers of axons in spared white matter bridges, microglia, astrocytes or serotinergic fibres. However, myelotomy reduced synaptophysin expression, a marker of synaptic plasticity. We conclude that further studies are required to evaluate myelotomy after SCI. (142 words).",

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AU - Meyer, Carolin

AU - Bendella, Habib

AU - Rink, Svenja

AU - Gensch, Robin

AU - Seitz, Robert

AU - Stein, Gregor

AU - Manthou, Marilena

AU - Papamitsou, Theodora

AU - Nakamura, Makoto

AU - Bouillon, Bertil

AU - Galea, Mary

AU - Batchelor, Peter

AU - Dunlop, Sarah

AU - Angelov, Doychin

PY - 2018/8/1

Y1 - 2018/8/1

N2 - Myelotomy is a surgical procedure allowing removal of extravasated blood and necrotic tissue that is thought to attenuate secondary injury as well as promote recovery in experimental spinal cord injury (SCI) models and humans. Here we examined in rat whether myelotomy at 48 h after low-thoracic compressive SCI provided any benefit over a 12 week period. Compared to animals receiving SCI alone, myelotomy worsened BBB scores (p < 0.05) and also did not improve plantar stepping, ladder climbing, urinary bladder voiding or sensory function (thermal latency) during the 12-week period. Quantitative analyses of tissue sections at 12 weeks showed that myelotomy also did not reduce lesion volume nor alter immunohistochemical markers of axons in spared white matter bridges, microglia, astrocytes or serotinergic fibres. However, myelotomy reduced synaptophysin expression, a marker of synaptic plasticity. We conclude that further studies are required to evaluate myelotomy after SCI. (142 words).

AB - Myelotomy is a surgical procedure allowing removal of extravasated blood and necrotic tissue that is thought to attenuate secondary injury as well as promote recovery in experimental spinal cord injury (SCI) models and humans. Here we examined in rat whether myelotomy at 48 h after low-thoracic compressive SCI provided any benefit over a 12 week period. Compared to animals receiving SCI alone, myelotomy worsened BBB scores (p < 0.05) and also did not improve plantar stepping, ladder climbing, urinary bladder voiding or sensory function (thermal latency) during the 12-week period. Quantitative analyses of tissue sections at 12 weeks showed that myelotomy also did not reduce lesion volume nor alter immunohistochemical markers of axons in spared white matter bridges, microglia, astrocytes or serotinergic fibres. However, myelotomy reduced synaptophysin expression, a marker of synaptic plasticity. We conclude that further studies are required to evaluate myelotomy after SCI. (142 words).

KW - Bladder and sensory function

KW - Cellular markers

KW - Locomotor

KW - Myelotomy

KW - Rat

KW - Spinal cord injury

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SN - 0014-4886

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JO - Experimental Neurology

JF - Experimental Neurology

ER -

The effect of myelotomy following low thoracic spinal cord compression injury in rats

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