The effect of cell density, conditioned medium and cytosine arabinoside on myogenesis in primary and secondary cultures

G. C.T. Yeoh, H. Holtzer

Research output: Contribution to journalArticle

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Abstract

Fusion in myogenic cultures is followed by direct microscopic observation and by monitoring the accumulation of creatine phosphokinase (CPK). CPK levels are useful when dealing with cultures in early stages of fusion and in high density cultures, for under these circumstances reliable estimates effusion are impossible to obtain microscopically. The proposition tested is that (i) cell density; (ii) conditioned medium and (iii) cytosine arabinoside can induce precocious fusion in a population of myogenic cells. We find we cannot induce precocious fusion by varying the above exogenous conditions in cells which are not endogenously programmed to fuse. Our data are consistent with the observation that there are two major subpopulations of myogenic cells which, in terms of cell division and fusion, are restricted in their options. There are the replicating presumptive myoblasts which cannot fuse, and the post-mitotic myoblasts which are the only cells in the myogenic lineage that can fuse. These two types of myogenic cells constitute the penultimate and ultimate compartments of the myogenic lineage.

Original languageEnglish
Pages (from-to)63-78
Number of pages16
JournalExperimental Cell Research
Volume104
Issue number1
DOIs
Publication statusPublished - 1 Jan 1977
Externally publishedYes

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Muscle Development
Cytarabine
Conditioned Culture Medium
Cell Count
Myoblasts
Creatine Kinase
Cell Fusion
Cell Division
Population

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abstract = "Fusion in myogenic cultures is followed by direct microscopic observation and by monitoring the accumulation of creatine phosphokinase (CPK). CPK levels are useful when dealing with cultures in early stages of fusion and in high density cultures, for under these circumstances reliable estimates effusion are impossible to obtain microscopically. The proposition tested is that (i) cell density; (ii) conditioned medium and (iii) cytosine arabinoside can induce precocious fusion in a population of myogenic cells. We find we cannot induce precocious fusion by varying the above exogenous conditions in cells which are not endogenously programmed to fuse. Our data are consistent with the observation that there are two major subpopulations of myogenic cells which, in terms of cell division and fusion, are restricted in their options. There are the replicating presumptive myoblasts which cannot fuse, and the post-mitotic myoblasts which are the only cells in the myogenic lineage that can fuse. These two types of myogenic cells constitute the penultimate and ultimate compartments of the myogenic lineage.",
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The effect of cell density, conditioned medium and cytosine arabinoside on myogenesis in primary and secondary cultures. / Yeoh, G. C.T.; Holtzer, H.

In: Experimental Cell Research, Vol. 104, No. 1, 01.01.1977, p. 63-78.

Research output: Contribution to journalArticle

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AU - Yeoh, G. C.T.

AU - Holtzer, H.

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AB - Fusion in myogenic cultures is followed by direct microscopic observation and by monitoring the accumulation of creatine phosphokinase (CPK). CPK levels are useful when dealing with cultures in early stages of fusion and in high density cultures, for under these circumstances reliable estimates effusion are impossible to obtain microscopically. The proposition tested is that (i) cell density; (ii) conditioned medium and (iii) cytosine arabinoside can induce precocious fusion in a population of myogenic cells. We find we cannot induce precocious fusion by varying the above exogenous conditions in cells which are not endogenously programmed to fuse. Our data are consistent with the observation that there are two major subpopulations of myogenic cells which, in terms of cell division and fusion, are restricted in their options. There are the replicating presumptive myoblasts which cannot fuse, and the post-mitotic myoblasts which are the only cells in the myogenic lineage that can fuse. These two types of myogenic cells constitute the penultimate and ultimate compartments of the myogenic lineage.

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