The Diversity and Distribution of Viruses Associated with Culex annulirostris Mosquitoes from the Kimberley Region of Western Australia

Simon H. Williams; Avram Levy; Rachel A. Yates; Nilusha Somaweera; Peter J. Neville; Jay Nicholson; Michael D.A. Lindsay; John S. Mackenzie; Komal Jain; Allison Imrie; David W. Smith; W. Ian Lipkin

doi:10.3390/v12070717

The Diversity and Distribution of Viruses Associated with Culex annulirostris Mosquitoes from the Kimberley Region of Western Australia

Simon H. Williams, Avram Levy, Rachel A. Yates, Nilusha Somaweera, Peter J. Neville, Jay Nicholson, Michael D.A. Lindsay, John S. Mackenzie, Komal Jain, Allison Imrie, David W. Smith, W. Ian Lipkin

Research output: Contribution to journal › Article › peer-review

8 Citations (Scopus)

Abstract

Metagenomics revealed an impressive breadth of previously unrecognized viruses. Here, we report the virome of the Culex annulirostris Skuse mosquito, an important vector of pathogenic arboviruses in Australia. Mosquitoes were collected from three sites in the Kimberley region of Western Australia. Unbiased high-throughput sequencing (HTS) revealed the presence of 16 novel viral sequences that share less than 90% identity with known viruses. None were closely related to pathogenic arboviruses. Viruses were distributed unevenly across sites, indicating a heterogeneous Cx. annulirostris virome. Polymerase chain reaction assays confirmed HTS data and identified marked variation between the virus prevalence identified at each site.

Original language	English
Article number	717
Journal	Viruses
Volume	12
Issue number	7
DOIs	https://doi.org/10.3390/v12070717
Publication status	Published - Jul 2020

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.3390/v12070717Licence: CC BY

Cite this

Williams, S. H., Levy, A., Yates, R. A., Somaweera, N., Neville, P. J., Nicholson, J., Lindsay, M. D. A., Mackenzie, J. S., Jain, K., Imrie, A., Smith, D. W., & Lipkin, W. I. (2020). The Diversity and Distribution of Viruses Associated with Culex annulirostris Mosquitoes from the Kimberley Region of Western Australia. Viruses, 12(7), [717]. https://doi.org/10.3390/v12070717

@article{447362c7cd394f22bf6ff466a85fa018,

title = "The Diversity and Distribution of Viruses Associated with Culex annulirostris Mosquitoes from the Kimberley Region of Western Australia",

abstract = "Metagenomics revealed an impressive breadth of previously unrecognized viruses. Here, we report the virome of the Culex annulirostris Skuse mosquito, an important vector of pathogenic arboviruses in Australia. Mosquitoes were collected from three sites in the Kimberley region of Western Australia. Unbiased high-throughput sequencing (HTS) revealed the presence of 16 novel viral sequences that share less than 90% identity with known viruses. None were closely related to pathogenic arboviruses. Viruses were distributed unevenly across sites, indicating a heterogeneous Cx. annulirostris virome. Polymerase chain reaction assays confirmed HTS data and identified marked variation between the virus prevalence identified at each site. ",

keywords = "Culex annulirostris, virome, Western Australia",

author = "Williams, {Simon H.} and Avram Levy and Yates, {Rachel A.} and Nilusha Somaweera and Neville, {Peter J.} and Jay Nicholson and Lindsay, {Michael D.A.} and Mackenzie, {John S.} and Komal Jain and Allison Imrie and Smith, {David W.} and Lipkin, {W. Ian}",

year = "2020",

month = jul,

doi = "10.3390/v12070717",

language = "English",

volume = "12",

journal = "Viruses",

issn = "1999-4915",

publisher = "MDPI Multidisciplinary Digital Publishing Institute",

number = "7",

}

TY - JOUR

T1 - The Diversity and Distribution of Viruses Associated with Culex annulirostris Mosquitoes from the Kimberley Region of Western Australia

AU - Williams, Simon H.

AU - Levy, Avram

AU - Yates, Rachel A.

AU - Somaweera, Nilusha

AU - Neville, Peter J.

AU - Nicholson, Jay

AU - Lindsay, Michael D.A.

AU - Mackenzie, John S.

AU - Jain, Komal

AU - Imrie, Allison

AU - Smith, David W.

AU - Lipkin, W. Ian

PY - 2020/7

Y1 - 2020/7

N2 - Metagenomics revealed an impressive breadth of previously unrecognized viruses. Here, we report the virome of the Culex annulirostris Skuse mosquito, an important vector of pathogenic arboviruses in Australia. Mosquitoes were collected from three sites in the Kimberley region of Western Australia. Unbiased high-throughput sequencing (HTS) revealed the presence of 16 novel viral sequences that share less than 90% identity with known viruses. None were closely related to pathogenic arboviruses. Viruses were distributed unevenly across sites, indicating a heterogeneous Cx. annulirostris virome. Polymerase chain reaction assays confirmed HTS data and identified marked variation between the virus prevalence identified at each site.

AB - Metagenomics revealed an impressive breadth of previously unrecognized viruses. Here, we report the virome of the Culex annulirostris Skuse mosquito, an important vector of pathogenic arboviruses in Australia. Mosquitoes were collected from three sites in the Kimberley region of Western Australia. Unbiased high-throughput sequencing (HTS) revealed the presence of 16 novel viral sequences that share less than 90% identity with known viruses. None were closely related to pathogenic arboviruses. Viruses were distributed unevenly across sites, indicating a heterogeneous Cx. annulirostris virome. Polymerase chain reaction assays confirmed HTS data and identified marked variation between the virus prevalence identified at each site.

KW - Culex annulirostris

KW - virome

KW - Western Australia

UR - http://www.scopus.com/inward/record.url?scp=85087687651&partnerID=8YFLogxK

U2 - 10.3390/v12070717

DO - 10.3390/v12070717

M3 - Article

C2 - 32630711

AN - SCOPUS:85087687651

VL - 12

JO - Viruses

JF - Viruses

SN - 1999-4915

IS - 7

M1 - 717

ER -

The Diversity and Distribution of Viruses Associated with Culex annulirostris Mosquitoes from the Kimberley Region of Western Australia

Abstract

UN SDGs

Access to Document

Other files and links

Fingerprint

Cite this