The Distribution of Arsenate and Arsenite in Shoots and Roots of Holcus lanatus is Influenced by Arsenic Tolerance and Arsenate and Phosphate Supply

M. Quaghebeur, Zed Rengel

Research output: Contribution to journalArticlepeer-review

94 Citations (Scopus)

Abstract

The recent discovery that phytochelatins are important for arsenic (As) detoxification in terrestrial plants results in the necessity to understand As speciation and metabolism in plant material. A hydroponic study was therefore conducted to examine the effects of different levels of phosphate and arsenate [As(V)] on As speciation and distribution in tolerant and non-tolerant clones of Holcus lanatus. Speciation. of As in tissue (using high-performance liquid chromatography-inductively coupled plasma mass spectrometry) revealed that the predominant species present were the inorganic As species (As(V) and arsenite [As(Ill)]), although small levels (<1%) of organic As species (dimethylarsinic acid and monomethylarsonic acid) were detected in shoot material. In roots, the proportion of total As present as As(III) generally increased with increasing levels of As(V) in the nutrient solution, whereas in shoots, the proportion of total As present as As(III) generally decreased with increasing levels of As(V). H. lanatus plants growing in the high-phosphorus (P) (100 &mu;m) solution contained a higher proportion of As(V) (with regard to total As) in both roots and shoots than plants supplied with low P (10 &mu;m); in addition, tolerant clones generally contained a higher proportion of As(V) with regard to total As than non-tolerant clones. The study further revealed that As(V) can be reduced to As(III) in both roots and shoots. Although the reduction capacity was limited, the reduction was closely regulated by As influx for all treatments. The results therefore provide a new understanding about As metabolism in H. lanatus.
Original languageEnglish
Pages (from-to)1600-1609
JournalPlant Physiology
Volume132
Issue number3
DOIs
Publication statusPublished - 2003

Fingerprint

Dive into the research topics of 'The Distribution of Arsenate and Arsenite in Shoots and Roots of Holcus lanatus is Influenced by Arsenic Tolerance and Arsenate and Phosphate Supply'. Together they form a unique fingerprint.

Cite this