[Truncated] Forensic DNA profiling has become an indispensable and routine investigative tool for the purpose of human identification in criminal justice and elsewhere. DNA profiling utilises the highly sensitive Polymerase Chain Reaction (PCR) to amplify the polymorphic microsatellite DNA and produce a pattern or profile of the genetic material from blood or cellular samples. The resultant DNA profile can be used for identification purposes and to distinguish between individuals. In current DNA profiling one of the most serious sources of contamination is carryover contamination resulting from the accidental transfer of PCR amplicons produced during the DNA profiling reaction into fresh samples. Although accidental laboratory carryover contamination by PCR amplicons is widely recognised, the issue of intentional contamination of samples has not previously been considered. As new technologies to improve the power and sensitivity of DNA profiling technology are introduced, the accidental contamination of field or laboratory samples will become a major issue.
|Publication status||Unpublished - 2007|