The detection and characterisation of forensic DNA profiles manipulated by the addition of PCR amplicons

Rebecca Jane Dent

    Research output: ThesisMaster's Thesis

    26 Downloads (Pure)

    Abstract

    [Truncated] Forensic DNA profiling has become an indispensable and routine investigative tool for the purpose of human identification in criminal justice and elsewhere. DNA profiling utilises the highly sensitive Polymerase Chain Reaction (PCR) to amplify the polymorphic microsatellite DNA and produce a pattern or profile of the genetic material from blood or cellular samples. The resultant DNA profile can be used for identification purposes and to distinguish between individuals. In current DNA profiling one of the most serious sources of contamination is carryover contamination resulting from the accidental transfer of PCR amplicons produced during the DNA profiling reaction into fresh samples. Although accidental laboratory carryover contamination by PCR amplicons is widely recognised, the issue of intentional contamination of samples has not previously been considered. As new technologies to improve the power and sensitivity of DNA profiling technology are introduced, the accidental contamination of field or laboratory samples will become a major issue.
    Original languageEnglish
    QualificationMasters
    Awarding Institution
    • The University of Western Australia
    DOIs
    Publication statusUnpublished - 2007

      Fingerprint

    Bibliographical note

    This thesis has been made available in the UWA Profiles and Research Repository as part of a UWA Library project to digitise and make available theses completed before 2003. If you are the author of this thesis and would like it removed from the UWA Profiles and Research Repository, please contact digitaltheses-lib@uwa.edu.au

    Cite this