The cytotoxic lipid peroxidation product, 4-hydroxy-2-nonenal, specifically inhibits decarboxylating dehydrogenases in the matrix of plant mitochondria

Harvey Millar, C.J. Leaver

Research output: Contribution to journalArticle

86 Citations (Scopus)

Abstract

4-Hydroxy-2-nonenal (HNE), a cytotoxic product of lipid peroxidation, inhibits O-2 consumption by potato tuber mitochondria, 2-Oxoglutarate dehydrogenase (OGDC), pyruvate dehydrogenase complex (PDC) (both 80% inhibited) and NAD-malic enzyme (50% inhibited) are its major targets. Mitochondrial proteins identified by reaction with antibodies raised to lipoic acid lost this antigenicity following HNE treatment. These proteins were identified as acetyltransferases of PDC (78 kDa and 55 kDa), succinyltransferases of OGDC (50 kDa and 48 kDa) and glycine decarboxylase H protein (17 kDa), The significance of the effect of these inhibitions on the impact of lipid peroxidation and plant respiratory functions is discussed. (C) 2000 Federation of European Biochemical Societies, Published by Elsevier Science B.V. All rights reserved.
Original languageEnglish
Pages (from-to)117-121
JournalFEBS Letters
Volume481
DOIs
Publication statusPublished - 2000

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