Temporal flux in ß-lactam resistance among Klebsiella pneumoniae in Western Australia

Jarrad M. Hall, Paul R. Ingram, L.C. O’Reilly, Tim J.J. Inglis

    Research output: Contribution to journalArticle

    5 Citations (Scopus)

    Abstract

    © 2016 The Authors Printed in Great Britain. Our aim was to identify long-term ß-lactam resistance trends in local Klebsiella pneumoniae isolates, which are a common cause of sepsis in Western Australia. We studied three collections of K. pneumoniae isolates from Western Australia between 1977 and 2015 comprising contemporary blood culture (n=98), multiresistant (n=21) and historical (n =50) isolates. Antimicrobial resistance was determined by Clinical and Laboratory Standards Institute agar dilution methods. PCR DNA sequencing identified ß-lactamase variants and porin mutations contributing to ß-lactam resistance. Isolates were genotyped by PFGE, multilocus sequence typing and a variable number tandem repeat method. From 1989 onwards, we detected the SHV-2a extended-spectrum ß-lactamase (ESBL) in ceftriaxone-resistant isolates, and in ceftazidime-and aztreonam-resistant isolates from 1993. Ceftriaxone, ceftazidime and aztreonam resistance persisted, with blaCTX-M types becoming the dominant ESBLs by 2010. CTX-M-15 was encountered in both multiresistant and blood culture isolates. Meropenem resistance was detected for the first time in 2011 in a locally isolated blaIMP-4-positive K. pneumoniae. We found sequence types ST23 and ST86 that occurred in multiple isolates from invasive infections. ST86 was the most common and maintained a high degree (90%) of similarity by PFGE since 1977. Ceftazidime-resistant K. pneumoniae sequence types have caused invasive infections in Western Australia since 1993. Invasive isolates producing CTX-M-14 and CTX-M-15 appeared in Western Australia during the last decade, before the appearance of carbapenemases. The diversity of ß-lactam resistance and ß-lactamase resistance mechanisms in Western Australian K. pneumoniae has increased since ESBLs were first described locally.
    Original languageEnglish
    Pages (from-to)429-437
    Number of pages9
    JournalJournal of Medical Microbiology
    Volume65
    Issue number5
    DOIs
    Publication statusPublished - 2016

    Fingerprint

    Lactams
    Western Australia
    Klebsiella pneumoniae
    Ceftazidime
    Aztreonam
    Ceftriaxone
    meropenem
    Multilocus Sequence Typing
    Porins
    Minisatellite Repeats
    Infection
    DNA Sequence Analysis
    Agar
    Sepsis
    Polymerase Chain Reaction
    Mutation

    Cite this

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    title = "Temporal flux in {\ss}-lactam resistance among Klebsiella pneumoniae in Western Australia",
    abstract = "{\circledC} 2016 The Authors Printed in Great Britain. Our aim was to identify long-term {\ss}-lactam resistance trends in local Klebsiella pneumoniae isolates, which are a common cause of sepsis in Western Australia. We studied three collections of K. pneumoniae isolates from Western Australia between 1977 and 2015 comprising contemporary blood culture (n=98), multiresistant (n=21) and historical (n =50) isolates. Antimicrobial resistance was determined by Clinical and Laboratory Standards Institute agar dilution methods. PCR DNA sequencing identified {\ss}-lactamase variants and porin mutations contributing to {\ss}-lactam resistance. Isolates were genotyped by PFGE, multilocus sequence typing and a variable number tandem repeat method. From 1989 onwards, we detected the SHV-2a extended-spectrum {\ss}-lactamase (ESBL) in ceftriaxone-resistant isolates, and in ceftazidime-and aztreonam-resistant isolates from 1993. Ceftriaxone, ceftazidime and aztreonam resistance persisted, with blaCTX-M types becoming the dominant ESBLs by 2010. CTX-M-15 was encountered in both multiresistant and blood culture isolates. Meropenem resistance was detected for the first time in 2011 in a locally isolated blaIMP-4-positive K. pneumoniae. We found sequence types ST23 and ST86 that occurred in multiple isolates from invasive infections. ST86 was the most common and maintained a high degree (90{\%}) of similarity by PFGE since 1977. Ceftazidime-resistant K. pneumoniae sequence types have caused invasive infections in Western Australia since 1993. Invasive isolates producing CTX-M-14 and CTX-M-15 appeared in Western Australia during the last decade, before the appearance of carbapenemases. The diversity of {\ss}-lactam resistance and {\ss}-lactamase resistance mechanisms in Western Australian K. pneumoniae has increased since ESBLs were first described locally.",
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    Temporal flux in ß-lactam resistance among Klebsiella pneumoniae in Western Australia. / Hall, Jarrad M.; Ingram, Paul R.; O’Reilly, L.C.; Inglis, Tim J.J.

    In: Journal of Medical Microbiology, Vol. 65, No. 5, 2016, p. 429-437.

    Research output: Contribution to journalArticle

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    AU - Ingram, Paul R.

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