Temporal changes in BEXSERO® antigen sequence type associated with genetic lineages of Neisseria meningitidis over a 15-year period in Western Australia

Shakeel Mowlaboccus, Tim Perkins, H. Smith, T. Sloots, S. Tozer, Lydia Prempeh, Alfred Chin Yen Tay, Fanny Peters, D. Speers, A.D. Keil, Charlene Kahler

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    Abstract

    © 2016 Mowlaboccus et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.Neisseria meningitidis is the causative agent of invasive meningococcal disease (IMD). The BEXSERO® vaccine which is used to prevent serogroup B disease is composed of four sub-capsular protein antigens supplemented with an outer membrane vesicle. Since the sub-capsular protein antigens are variably expressed and antigenically variable amongst meningococcal isolates, vaccine coverage can be estimated by the meningococcal antigen typing system (MATS) which measures the propensity of the strain to be killed by vaccinated sera. Whole genome sequencing (WGS) which identifies the alleles of the antigens that may be recognised by the antibody response could represent, in future, an alternative estimate of coverage. In this study, WGS of 278 meningococcal isolates responsible for 62% of IMD in Western Australia from 2000-2014 were analysed for association of genetic lineage (sequence type [ST], clonal complex [cc]) with BEXSERO® antigen sequence type (BAST) and MATS to predict the annual vaccine coverage. A hyperendemic period of IMD between 2000-05 was caused by cc41/44 with the major sequence type of ST-146 which was not predicted by MATS or BAST to be covered by the vaccine. An increase in serogroup diversity was observed between 2010-14 with the emergence of cc11 serogroup W in the adolescent population and cc23 serogroup Y in the elderly. BASTs were statistically associated with clonal complex although individual antigens underwent antigenic drift from the major type. BAST and MATS predicted an annual range of 44-91% vaccine coverage. Periods of low vaccine coverage in years post-2005 were not a result of the resurgence of cc41/44:ST-146 but were characterised by increased diversity of clonal complexes expressing BASTs which were not predicted by MATS to be c
    Original languageEnglish
    JournalPLoS One
    Volume11
    Issue number6
    DOIs
    Publication statusPublished - 29 Jun 2016

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    Neisseria meningitidis
    Western Australia
    antigens
    Antigens
    vaccines
    Vaccines
    serotypes
    Genes
    Meningococcal Vaccines
    Genome
    antigenic variation
    genome
    Licensure
    Antibody Formation
    Reproduction
    Proteins

    Cite this

    Mowlaboccus, Shakeel ; Perkins, Tim ; Smith, H. ; Sloots, T. ; Tozer, S. ; Prempeh, Lydia ; Tay, Alfred Chin Yen ; Peters, Fanny ; Speers, D. ; Keil, A.D. ; Kahler, Charlene. / Temporal changes in BEXSERO® antigen sequence type associated with genetic lineages of Neisseria meningitidis over a 15-year period in Western Australia. In: PLoS One. 2016 ; Vol. 11, No. 6.
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    abstract = "{\circledC} 2016 Mowlaboccus et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.Neisseria meningitidis is the causative agent of invasive meningococcal disease (IMD). The BEXSERO{\circledR} vaccine which is used to prevent serogroup B disease is composed of four sub-capsular protein antigens supplemented with an outer membrane vesicle. Since the sub-capsular protein antigens are variably expressed and antigenically variable amongst meningococcal isolates, vaccine coverage can be estimated by the meningococcal antigen typing system (MATS) which measures the propensity of the strain to be killed by vaccinated sera. Whole genome sequencing (WGS) which identifies the alleles of the antigens that may be recognised by the antibody response could represent, in future, an alternative estimate of coverage. In this study, WGS of 278 meningococcal isolates responsible for 62{\%} of IMD in Western Australia from 2000-2014 were analysed for association of genetic lineage (sequence type [ST], clonal complex [cc]) with BEXSERO{\circledR} antigen sequence type (BAST) and MATS to predict the annual vaccine coverage. A hyperendemic period of IMD between 2000-05 was caused by cc41/44 with the major sequence type of ST-146 which was not predicted by MATS or BAST to be covered by the vaccine. An increase in serogroup diversity was observed between 2010-14 with the emergence of cc11 serogroup W in the adolescent population and cc23 serogroup Y in the elderly. BASTs were statistically associated with clonal complex although individual antigens underwent antigenic drift from the major type. BAST and MATS predicted an annual range of 44-91{\%} vaccine coverage. Periods of low vaccine coverage in years post-2005 were not a result of the resurgence of cc41/44:ST-146 but were characterised by increased diversity of clonal complexes expressing BASTs which were not predicted by MATS to be c",
    author = "Shakeel Mowlaboccus and Tim Perkins and H. Smith and T. Sloots and S. Tozer and Lydia Prempeh and Tay, {Alfred Chin Yen} and Fanny Peters and D. Speers and A.D. Keil and Charlene Kahler",
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    Temporal changes in BEXSERO® antigen sequence type associated with genetic lineages of Neisseria meningitidis over a 15-year period in Western Australia. / Mowlaboccus, Shakeel; Perkins, Tim; Smith, H.; Sloots, T.; Tozer, S.; Prempeh, Lydia; Tay, Alfred Chin Yen; Peters, Fanny; Speers, D.; Keil, A.D.; Kahler, Charlene.

    In: PLoS One, Vol. 11, No. 6, 29.06.2016.

    Research output: Contribution to journalArticle

    TY - JOUR

    T1 - Temporal changes in BEXSERO® antigen sequence type associated with genetic lineages of Neisseria meningitidis over a 15-year period in Western Australia

    AU - Mowlaboccus, Shakeel

    AU - Perkins, Tim

    AU - Smith, H.

    AU - Sloots, T.

    AU - Tozer, S.

    AU - Prempeh, Lydia

    AU - Tay, Alfred Chin Yen

    AU - Peters, Fanny

    AU - Speers, D.

    AU - Keil, A.D.

    AU - Kahler, Charlene

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