Telomerecat: A ploidy-agnostic method for estimating telomere length from whole genome sequencing data

James H.R. Farmery, Mike L. Smith, Aarnoud Huissoon, Abigail Furnell, Adam Mead, Adam P. Levine, Adnan Manzur, Adrian Thrasher, Alan Greenhalgh, Alasdair Parker, Alba Sanchis-Juan, Alex Richter, Alice Gardham, Allan Lawrie, Aman Sohal, Amanda Creaser-Myers, Amy Frary, Andreas Greinacher, Andreas Themistocleous, Andrew J. Peacock & 329 others Andrew Marshall, Andrew Mumford, Andrew Rice, Andrew Webster, Angie Brady, Ania Koziell, Ania Manson, Anita Chandra, Anke Hensiek, Anna Huis In T. Veld, Anna Maw, Anne M. Kelly, Anthony Moore, Anton Vonk Noordegraaf, Antony Attwood, Archana Herwadkar, Ardi Ghofrani, Arjan C. Houweling, Barbara Girerd, Bruce Furie, Carmen M. Treacy, Carolyn M. Millar, Carrock Sewell, Catherine Roughley, Catherine Titterton, Catherine Williamson, Charaka Hadinnapola, Charu Deshpande, Cheng Hock Toh, Chiara Bacchelli, Chris Patch, Chris Van Geet, Christian Babbs, Christine Bryson, Christopher J. Penkett, Christopher J. Rhodes, Christopher Watt, Claire Bethune, Claire Booth, Claire Lentaigne, Coleen McJannet, Colin Church, Courtney French, Crina Samarghitean, Csaba Halmagyi, Daniel Gale, Daniel Greene, Daniel Hart, David Allsup, David Bennett, David Edgar, David G. Kiely, David Gosal, David J. Perry, David Keeling, David Montani, Debbie Shipley, Deborah Whitehorn, Debra Fletcher, Deepa Krishnakumar, Detelina Grozeva, Dinakantha Kumararatne, Dorothy Thompson, Dragana Josifova, Eamonn Maher, Edwin K.S. Wong, Elaine Murphy, Eleanor Dewhurst, Eleni Louka, Elisabeth Rosser, Elizabeth Chalmers, Elizabeth Colby, Elizabeth Drewe, Elizabeth McDermott, Ellen Thomas, Emily Staples, Emma Clement, Emma Matthews, Emma Wakeling, Eric Oksenhendler, Ernest Turro, Evan Reid, Evangeline Wassmer, F. Lucy Raymond, Fengyuan Hu, Fiona Kennedy, Florent Soubrier, Frances Flinter, Gabor Kovacs, Gary Polwarth, Gautum Ambegaonkar, Gavin Arno, Gavin Hudson, Geoff Woods, Gerry Coghlan, Grant Hayman, Gururaj Arumugakani, Gwen Schotte, H. Terry Cook, Hana Alachkar, Hana Lango Allen, Hana Lango-Allen, Hannah Stark, Hans Stauss, Harald Schulze, Harm J. Boggard, Helen Baxendale, Helen Dolling, Helen Firth, Henning Gall, Henry Watson, Hilary Longhurst, Hugh S. Markus, Hugh Watkins, Ilenia Simeoni, Ingrid Emmerson, Irene Roberts, Isabella Quinti, Ivy Wanjiku, J. Simon R. Gibbs, James Thaventhiran, James Whitworth, Jane Hurst, Janine Collins, Jay Suntharalingam, Jeanette Payne, Jecko Thachil, Jennifer M. Martin, Jenny Carmichael, Jesmeen Maimaris, Joan Paterson, Joanna Pepke-Zaba, Johan W.M. Heemskerk, Johanna Gebhart, John Davis, John Pasi, John R. Bradley, John Wharton, Jonathan Stephens, Julia Rankin, Julie Anderson, Julie Vogt, Julie Von Ziegenweldt, Karola Rehnstrom, Karyn Megy, Kate Talks, Kathelijne Peerlinck, Katherine Yates, Kathleen Freson, Kathleen Stirrups, Keith Gomez, Kenneth G.C. Smith, Kevin Rue-Albrecht, Kimberley Gilmour, Larahmie Masati, Laura Scelsi, Laura Southgate, Lavanya Ranganathan, Lionel Ginsberg, Lisa Devlin, Lisa Willcocks, Liz Ormondroyd, Lorena Lorenzo, Lorraine Harper, Louise Allen, Louise Daugherty, Manali Chitre, Manju Kurian, Marc Humbert, Marc Tischkowitz, Maria Bitner-Glindzicz, Marie Erwood, Marie Scully, Marijke Veltman, Mark Caulfield, Mark Layton, Mark McCarthy, Mark Ponsford, Mark Toshner, Marta Bleda, Martin Wilkins, Mary Mathias, Mary Reilly, Maryam Afzal, Matthew Brown, Matthew Rondina, Matthew Stubbs, Matthias Haimel, Melissa Lees, Michael A. Laffan, Michael Browning, Michael Gattens, Michael Richards, Michel Michaelides, Michele P. Lambert, Mike Makris, Minka De Vries, Mohamed Mahdi-Rogers, Moin Saleem, Moira Thomas, Muriel Holder, Mélanie Eyries, Naomi Clements-Brod, Natalie Canham, Natalie Dormand, Natalie Van Zuydam, Nathalie Kingston, Neeti Ghali, Nichola Cooper, Nicholas W. Morrell, Nigel Yeatman, Noémi Roy, Olga Shamardina, Omid S. Alavijeh, Paolo Gresele, Paquita Nurden, Patrick Chinnery, Patrick Deegan, Patrick Yong, Patrick Yu Wai Man, Paul A. Corris, Paul Calleja, Paul Gissen, Paula Bolton-Maggs, Paula Rayner-Matthews, Pavandeep K. Ghataorhe, Pavel Gordins, Penelope Stein, Peter Collins, Peter Dixon, Peter Kelleher, Phil Ancliff, Ping Yu, R. Campbell Tait, Rachel Linger, Rainer Doffinger, Rajiv Machado, Rashid Kazmi, Ravishankar Sargur, Remi Favier, Rhea Tan, Ri Liesner, Richard Antrobus, Richard Sandford, Richard Scott, Richard Trembath, Rita Horvath, Rob Hadden, Rob V. Mackenzieross, Robert Henderson, Robert MacLaren, Roger James, Rohit Ghurye, Rosa Dacosta, Rosie Hague, Rutendo Mapeta, Ruth Armstrong, Sadia Noorani, Sai Murng, Saikat Santra, Salih Tuna, Sally Johnson, Sam Chong, Sara Lear, Sara Walker, Sarah Goddard, Sarah Mangles, Sarah Westbury, Sarju Mehta, Scott Hackett, Sergey Nejentsev, Shahin Moledina, Shahnaz Bibi, Sharon Meehan, Shokri Othman, Shoshana Revel-Vilk, Simon Holden, Simon McGowan, Simon Staines, Sinisa Savic, Siobhan Burns, Sofia Grigoriadou, Sofia Papadia, Sofie Ashford, Sol Schulman, Sonia Ali, Soo Mi Park, Sophie Davies, Sophie Stock, Souad Ali, Sri V.V. Deevi, Stefan Gräf, Stefano Ghio, Stephen J. Wort, Stephen Jolles, Steve Austin, Steve Welch, Stuart Meacham, Stuart Rankin, Suellen Walker, Suranjith Seneviratne, Susan Holder, Suthesh Sivapalaratnam, Sylvia Richardson, Taco W. Kuijpers, Taco W. Kuijpers, Tadbir K. Bariana, Tamam Bakchoul, Tamara Everington, Tara Renton, Tim Young, Timothy Aitman, Timothy Q. Warner, Tom Vale, Tracey Hammerton, Val Pollock, Vera Matser, Victoria Cookson, Virginia Clowes, Waseem Qasim, Wei Wei, Wendy N. Erber, Willem H. Ouwehand, William Astle, William Egner, Wojciech Turek, Yvonne Henskens, Yvonne Tan, Andy G. Lynch

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Telomere length is a risk factor in disease and the dynamics of telomere length are crucial to our understanding of cell replication and vitality. The proliferation of whole genome sequencing represents an unprecedented opportunity to glean new insights into telomere biology on a previously unimaginable scale. To this end, a number of approaches for estimating telomere length from whole-genome sequencing data have been proposed. Here we present Telomerecat, a novel approach to the estimation of telomere length. Previous methods have been dependent on the number of telomeres present in a cell being known, which may be problematic when analysing aneuploid cancer data and non-human samples. Telomerecat is designed to be agnostic to the number of telomeres present, making it suited for the purpose of estimating telomere length in cancer studies. Telomerecat also accounts for interstitial telomeric reads and presents a novel approach to dealing with sequencing errors. We show that Telomerecat performs well at telomere length estimation when compared to leading experimental and computational methods. Furthermore, we show that it detects expected patterns in longitudinal data, repeated measurements, and cross-species comparisons. We also apply the method to a cancer cell data, uncovering an interesting relationship with the underlying telomerase genotype.

Original languageEnglish
Article number1300
JournalScientific Reports
Volume8
Issue number1
DOIs
Publication statusPublished - 1 Dec 2018

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Ploidies
Telomere
Genome
Neoplasms
Telomerase
Aneuploidy
Genotype

Cite this

Farmery, J. H. R., Smith, M. L., Huissoon, A., Furnell, A., Mead, A., Levine, A. P., ... Lynch, A. G. (2018). Telomerecat: A ploidy-agnostic method for estimating telomere length from whole genome sequencing data. Scientific Reports, 8(1), [1300]. https://doi.org/10.1038/s41598-017-14403-y
Farmery, James H.R. ; Smith, Mike L. ; Huissoon, Aarnoud ; Furnell, Abigail ; Mead, Adam ; Levine, Adam P. ; Manzur, Adnan ; Thrasher, Adrian ; Greenhalgh, Alan ; Parker, Alasdair ; Sanchis-Juan, Alba ; Richter, Alex ; Gardham, Alice ; Lawrie, Allan ; Sohal, Aman ; Creaser-Myers, Amanda ; Frary, Amy ; Greinacher, Andreas ; Themistocleous, Andreas ; Peacock, Andrew J. ; Marshall, Andrew ; Mumford, Andrew ; Rice, Andrew ; Webster, Andrew ; Brady, Angie ; Koziell, Ania ; Manson, Ania ; Chandra, Anita ; Hensiek, Anke ; Veld, Anna Huis In T. ; Maw, Anna ; Kelly, Anne M. ; Moore, Anthony ; Vonk Noordegraaf, Anton ; Attwood, Antony ; Herwadkar, Archana ; Ghofrani, Ardi ; Houweling, Arjan C. ; Girerd, Barbara ; Furie, Bruce ; Treacy, Carmen M. ; Millar, Carolyn M. ; Sewell, Carrock ; Roughley, Catherine ; Titterton, Catherine ; Williamson, Catherine ; Hadinnapola, Charaka ; Deshpande, Charu ; Toh, Cheng Hock ; Bacchelli, Chiara ; Patch, Chris ; Geet, Chris Van ; Babbs, Christian ; Bryson, Christine ; Penkett, Christopher J. ; Rhodes, Christopher J. ; Watt, Christopher ; Bethune, Claire ; Booth, Claire ; Lentaigne, Claire ; McJannet, Coleen ; Church, Colin ; French, Courtney ; Samarghitean, Crina ; Halmagyi, Csaba ; Gale, Daniel ; Greene, Daniel ; Hart, Daniel ; Allsup, David ; Bennett, David ; Edgar, David ; Kiely, David G. ; Gosal, David ; Perry, David J. ; Keeling, David ; Montani, David ; Shipley, Debbie ; Whitehorn, Deborah ; Fletcher, Debra ; Krishnakumar, Deepa ; Grozeva, Detelina ; Kumararatne, Dinakantha ; Thompson, Dorothy ; Josifova, Dragana ; Maher, Eamonn ; Wong, Edwin K.S. ; Murphy, Elaine ; Dewhurst, Eleanor ; Louka, Eleni ; Rosser, Elisabeth ; Chalmers, Elizabeth ; Colby, Elizabeth ; Drewe, Elizabeth ; McDermott, Elizabeth ; Thomas, Ellen ; Staples, Emily ; Clement, Emma ; Matthews, Emma ; Wakeling, Emma ; Oksenhendler, Eric ; Turro, Ernest ; Reid, Evan ; Wassmer, Evangeline ; Raymond, F. Lucy ; Hu, Fengyuan ; Kennedy, Fiona ; Soubrier, Florent ; Flinter, Frances ; Kovacs, Gabor ; Polwarth, Gary ; Ambegaonkar, Gautum ; Arno, Gavin ; Hudson, Gavin ; Woods, Geoff ; Coghlan, Gerry ; Hayman, Grant ; Arumugakani, Gururaj ; Schotte, Gwen ; Cook, H. Terry ; Alachkar, Hana ; Lango Allen, Hana ; Lango-Allen, Hana ; Stark, Hannah ; Stauss, Hans ; Schulze, Harald ; Boggard, Harm J. ; Baxendale, Helen ; Dolling, Helen ; Firth, Helen ; Gall, Henning ; Watson, Henry ; Longhurst, Hilary ; Markus, Hugh S. ; Watkins, Hugh ; Simeoni, Ilenia ; Emmerson, Ingrid ; Roberts, Irene ; Quinti, Isabella ; Wanjiku, Ivy ; Gibbs, J. Simon R. ; Thaventhiran, James ; Whitworth, James ; Hurst, Jane ; Collins, Janine ; Suntharalingam, Jay ; Payne, Jeanette ; Thachil, Jecko ; Martin, Jennifer M. ; Carmichael, Jenny ; Maimaris, Jesmeen ; Paterson, Joan ; Pepke-Zaba, Joanna ; Heemskerk, Johan W.M. ; Gebhart, Johanna ; Davis, John ; Pasi, John ; Bradley, John R. ; Wharton, John ; Stephens, Jonathan ; Rankin, Julia ; Anderson, Julie ; Vogt, Julie ; Von Ziegenweldt, Julie ; Rehnstrom, Karola ; Megy, Karyn ; Talks, Kate ; Peerlinck, Kathelijne ; Yates, Katherine ; Freson, Kathleen ; Stirrups, Kathleen ; Gomez, Keith ; Smith, Kenneth G.C. ; Rue-Albrecht, Kevin ; Gilmour, Kimberley ; Masati, Larahmie ; Scelsi, Laura ; Southgate, Laura ; Ranganathan, Lavanya ; Ginsberg, Lionel ; Devlin, Lisa ; Willcocks, Lisa ; Ormondroyd, Liz ; Lorenzo, Lorena ; Harper, Lorraine ; Allen, Louise ; Daugherty, Louise ; Chitre, Manali ; Kurian, Manju ; Humbert, Marc ; Tischkowitz, Marc ; Bitner-Glindzicz, Maria ; Erwood, Marie ; Scully, Marie ; Veltman, Marijke ; Caulfield, Mark ; Layton, Mark ; McCarthy, Mark ; Ponsford, Mark ; Toshner, Mark ; Bleda, Marta ; Wilkins, Martin ; Mathias, Mary ; Reilly, Mary ; Afzal, Maryam ; Brown, Matthew ; Rondina, Matthew ; Stubbs, Matthew ; Haimel, Matthias ; Lees, Melissa ; Laffan, Michael A. ; Browning, Michael ; Gattens, Michael ; Richards, Michael ; Michaelides, Michel ; Lambert, Michele P. ; Makris, Mike ; De Vries, Minka ; Mahdi-Rogers, Mohamed ; Saleem, Moin ; Thomas, Moira ; Holder, Muriel ; Eyries, Mélanie ; Clements-Brod, Naomi ; Canham, Natalie ; Dormand, Natalie ; Zuydam, Natalie Van ; Kingston, Nathalie ; Ghali, Neeti ; Cooper, Nichola ; Morrell, Nicholas W. ; Yeatman, Nigel ; Roy, Noémi ; Shamardina, Olga ; Alavijeh, Omid S. ; Gresele, Paolo ; Nurden, Paquita ; Chinnery, Patrick ; Deegan, Patrick ; Yong, Patrick ; Man, Patrick Yu Wai ; Corris, Paul A. ; Calleja, Paul ; Gissen, Paul ; Bolton-Maggs, Paula ; Rayner-Matthews, Paula ; Ghataorhe, Pavandeep K. ; Gordins, Pavel ; Stein, Penelope ; Collins, Peter ; Dixon, Peter ; Kelleher, Peter ; Ancliff, Phil ; Yu, Ping ; Tait, R. Campbell ; Linger, Rachel ; Doffinger, Rainer ; Machado, Rajiv ; Kazmi, Rashid ; Sargur, Ravishankar ; Favier, Remi ; Tan, Rhea ; Liesner, Ri ; Antrobus, Richard ; Sandford, Richard ; Scott, Richard ; Trembath, Richard ; Horvath, Rita ; Hadden, Rob ; Mackenzieross, Rob V. ; Henderson, Robert ; MacLaren, Robert ; James, Roger ; Ghurye, Rohit ; Dacosta, Rosa ; Hague, Rosie ; Mapeta, Rutendo ; Armstrong, Ruth ; Noorani, Sadia ; Murng, Sai ; Santra, Saikat ; Tuna, Salih ; Johnson, Sally ; Chong, Sam ; Lear, Sara ; Walker, Sara ; Goddard, Sarah ; Mangles, Sarah ; Westbury, Sarah ; Mehta, Sarju ; Hackett, Scott ; Nejentsev, Sergey ; Moledina, Shahin ; Bibi, Shahnaz ; Meehan, Sharon ; Othman, Shokri ; Revel-Vilk, Shoshana ; Holden, Simon ; McGowan, Simon ; Staines, Simon ; Savic, Sinisa ; Burns, Siobhan ; Grigoriadou, Sofia ; Papadia, Sofia ; Ashford, Sofie ; Schulman, Sol ; Ali, Sonia ; Park, Soo Mi ; Davies, Sophie ; Stock, Sophie ; Ali, Souad ; Deevi, Sri V.V. ; Gräf, Stefan ; Ghio, Stefano ; Wort, Stephen J. ; Jolles, Stephen ; Austin, Steve ; Welch, Steve ; Meacham, Stuart ; Rankin, Stuart ; Walker, Suellen ; Seneviratne, Suranjith ; Holder, Susan ; Sivapalaratnam, Suthesh ; Richardson, Sylvia ; Kuijpers, Taco W. ; Kuijpers, Taco W. ; Bariana, Tadbir K. ; Bakchoul, Tamam ; Everington, Tamara ; Renton, Tara ; Young, Tim ; Aitman, Timothy ; Warner, Timothy Q. ; Vale, Tom ; Hammerton, Tracey ; Pollock, Val ; Matser, Vera ; Cookson, Victoria ; Clowes, Virginia ; Qasim, Waseem ; Wei, Wei ; Erber, Wendy N. ; Ouwehand, Willem H. ; Astle, William ; Egner, William ; Turek, Wojciech ; Henskens, Yvonne ; Tan, Yvonne ; Lynch, Andy G. / Telomerecat : A ploidy-agnostic method for estimating telomere length from whole genome sequencing data. In: Scientific Reports. 2018 ; Vol. 8, No. 1.
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title = "Telomerecat: A ploidy-agnostic method for estimating telomere length from whole genome sequencing data",
abstract = "Telomere length is a risk factor in disease and the dynamics of telomere length are crucial to our understanding of cell replication and vitality. The proliferation of whole genome sequencing represents an unprecedented opportunity to glean new insights into telomere biology on a previously unimaginable scale. To this end, a number of approaches for estimating telomere length from whole-genome sequencing data have been proposed. Here we present Telomerecat, a novel approach to the estimation of telomere length. Previous methods have been dependent on the number of telomeres present in a cell being known, which may be problematic when analysing aneuploid cancer data and non-human samples. Telomerecat is designed to be agnostic to the number of telomeres present, making it suited for the purpose of estimating telomere length in cancer studies. Telomerecat also accounts for interstitial telomeric reads and presents a novel approach to dealing with sequencing errors. We show that Telomerecat performs well at telomere length estimation when compared to leading experimental and computational methods. Furthermore, we show that it detects expected patterns in longitudinal data, repeated measurements, and cross-species comparisons. We also apply the method to a cancer cell data, uncovering an interesting relationship with the underlying telomerase genotype.",
author = "Farmery, {James H.R.} and Smith, {Mike L.} and Aarnoud Huissoon and Abigail Furnell and Adam Mead and Levine, {Adam P.} and Adnan Manzur and Adrian Thrasher and Alan Greenhalgh and Alasdair Parker and Alba Sanchis-Juan and Alex Richter and Alice Gardham and Allan Lawrie and Aman Sohal and Amanda Creaser-Myers and Amy Frary and Andreas Greinacher and Andreas Themistocleous and Peacock, {Andrew J.} and Andrew Marshall and Andrew Mumford and Andrew Rice and Andrew Webster and Angie Brady and Ania Koziell and Ania Manson and Anita Chandra and Anke Hensiek and Veld, {Anna Huis In T.} and Anna Maw and Kelly, {Anne M.} and Anthony Moore and {Vonk Noordegraaf}, Anton and Antony Attwood and Archana Herwadkar and Ardi Ghofrani and Houweling, {Arjan C.} and Barbara Girerd and Bruce Furie and Treacy, {Carmen M.} and Millar, {Carolyn M.} and Carrock Sewell and Catherine Roughley and Catherine Titterton and Catherine Williamson and Charaka Hadinnapola and Charu Deshpande and Toh, {Cheng Hock} and Chiara Bacchelli and Chris Patch and Geet, {Chris Van} and Christian Babbs and Christine Bryson and Penkett, {Christopher J.} and Rhodes, {Christopher J.} and Christopher Watt and Claire Bethune and Claire Booth and Claire Lentaigne and Coleen McJannet and Colin Church and Courtney French and Crina Samarghitean and Csaba Halmagyi and Daniel Gale and Daniel Greene and Daniel Hart and David Allsup and David Bennett and David Edgar and Kiely, {David G.} and David Gosal and Perry, {David J.} and David Keeling and David Montani and Debbie Shipley and Deborah Whitehorn and Debra Fletcher and Deepa Krishnakumar and Detelina Grozeva and Dinakantha Kumararatne and Dorothy Thompson and Dragana Josifova and Eamonn Maher and Wong, {Edwin K.S.} and Elaine Murphy and Eleanor Dewhurst and Eleni Louka and Elisabeth Rosser and Elizabeth Chalmers and Elizabeth Colby and Elizabeth Drewe and Elizabeth McDermott and Ellen Thomas and Emily Staples and Emma Clement and Emma Matthews and Emma Wakeling and Eric Oksenhendler and Ernest Turro and Evan Reid and Evangeline Wassmer and Raymond, {F. Lucy} and Fengyuan Hu and Fiona Kennedy and Florent Soubrier and Frances Flinter and Gabor Kovacs and Gary Polwarth and Gautum Ambegaonkar and Gavin Arno and Gavin Hudson and Geoff Woods and Gerry Coghlan and Grant Hayman and Gururaj Arumugakani and Gwen Schotte and Cook, {H. Terry} and Hana Alachkar and {Lango Allen}, Hana and Hana Lango-Allen and Hannah Stark and Hans Stauss and Harald Schulze and Boggard, {Harm J.} and Helen Baxendale and Helen Dolling and Helen Firth and Henning Gall and Henry Watson and Hilary Longhurst and Markus, {Hugh S.} and Hugh Watkins and Ilenia Simeoni and Ingrid Emmerson and Irene Roberts and Isabella Quinti and Ivy Wanjiku and Gibbs, {J. Simon R.} and James Thaventhiran and James Whitworth and Jane Hurst and Janine Collins and Jay Suntharalingam and Jeanette Payne and Jecko Thachil and Martin, {Jennifer M.} and Jenny Carmichael and Jesmeen Maimaris and Joan Paterson and Joanna Pepke-Zaba and Heemskerk, {Johan W.M.} and Johanna Gebhart and John Davis and John Pasi and Bradley, {John R.} and John Wharton and Jonathan Stephens and Julia Rankin and Julie Anderson and Julie Vogt and {Von Ziegenweldt}, Julie and Karola Rehnstrom and Karyn Megy and Kate Talks and Kathelijne Peerlinck and Katherine Yates and Kathleen Freson and Kathleen Stirrups and Keith Gomez and Smith, {Kenneth G.C.} and Kevin Rue-Albrecht and Kimberley Gilmour and Larahmie Masati and Laura Scelsi and Laura Southgate and Lavanya Ranganathan and Lionel Ginsberg and Lisa Devlin and Lisa Willcocks and Liz Ormondroyd and Lorena Lorenzo and Lorraine Harper and Louise Allen and Louise Daugherty and Manali Chitre and Manju Kurian and Marc Humbert and Marc Tischkowitz and Maria Bitner-Glindzicz and Marie Erwood and Marie Scully and Marijke Veltman and Mark Caulfield and Mark Layton and Mark McCarthy and Mark Ponsford and Mark Toshner and Marta Bleda and Martin Wilkins and Mary Mathias and Mary Reilly and Maryam Afzal and Matthew Brown and Matthew Rondina and Matthew Stubbs and Matthias Haimel and Melissa Lees and Laffan, {Michael A.} and Michael Browning and Michael Gattens and Michael Richards and Michel Michaelides and Lambert, {Michele P.} and Mike Makris and {De Vries}, Minka and Mohamed Mahdi-Rogers and Moin Saleem and Moira Thomas and Muriel Holder and M{\'e}lanie Eyries and Naomi Clements-Brod and Natalie Canham and Natalie Dormand and Zuydam, {Natalie Van} and Nathalie Kingston and Neeti Ghali and Nichola Cooper and Morrell, {Nicholas W.} and Nigel Yeatman and No{\'e}mi Roy and Olga Shamardina and Alavijeh, {Omid S.} and Paolo Gresele and Paquita Nurden and Patrick Chinnery and Patrick Deegan and Patrick Yong and Man, {Patrick Yu Wai} and Corris, {Paul A.} and Paul Calleja and Paul Gissen and Paula Bolton-Maggs and Paula Rayner-Matthews and Ghataorhe, {Pavandeep K.} and Pavel Gordins and Penelope Stein and Peter Collins and Peter Dixon and Peter Kelleher and Phil Ancliff and Ping Yu and Tait, {R. Campbell} and Rachel Linger and Rainer Doffinger and Rajiv Machado and Rashid Kazmi and Ravishankar Sargur and Remi Favier and Rhea Tan and Ri Liesner and Richard Antrobus and Richard Sandford and Richard Scott and Richard Trembath and Rita Horvath and Rob Hadden and Mackenzieross, {Rob V.} and Robert Henderson and Robert MacLaren and Roger James and Rohit Ghurye and Rosa Dacosta and Rosie Hague and Rutendo Mapeta and Ruth Armstrong and Sadia Noorani and Sai Murng and Saikat Santra and Salih Tuna and Sally Johnson and Sam Chong and Sara Lear and Sara Walker and Sarah Goddard and Sarah Mangles and Sarah Westbury and Sarju Mehta and Scott Hackett and Sergey Nejentsev and Shahin Moledina and Shahnaz Bibi and Sharon Meehan and Shokri Othman and Shoshana Revel-Vilk and Simon Holden and Simon McGowan and Simon Staines and Sinisa Savic and Siobhan Burns and Sofia Grigoriadou and Sofia Papadia and Sofie Ashford and Sol Schulman and Sonia Ali and Park, {Soo Mi} and Sophie Davies and Sophie Stock and Souad Ali and Deevi, {Sri V.V.} and Stefan Gr{\"a}f and Stefano Ghio and Wort, {Stephen J.} and Stephen Jolles and Steve Austin and Steve Welch and Stuart Meacham and Stuart Rankin and Suellen Walker and Suranjith Seneviratne and Susan Holder and Suthesh Sivapalaratnam and Sylvia Richardson and Kuijpers, {Taco W.} and Kuijpers, {Taco W.} and Bariana, {Tadbir K.} and Tamam Bakchoul and Tamara Everington and Tara Renton and Tim Young and Timothy Aitman and Warner, {Timothy Q.} and Tom Vale and Tracey Hammerton and Val Pollock and Vera Matser and Victoria Cookson and Virginia Clowes and Waseem Qasim and Wei Wei and Erber, {Wendy N.} and Ouwehand, {Willem H.} and William Astle and William Egner and Wojciech Turek and Yvonne Henskens and Yvonne Tan and Lynch, {Andy G.}",
year = "2018",
month = "12",
day = "1",
doi = "10.1038/s41598-017-14403-y",
language = "English",
volume = "8",
journal = "Scientific Reports",
issn = "2045-2322",
publisher = "Nature Publishing Group - Macmillan Publishers",
number = "1",

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Farmery, JHR, Smith, ML, Huissoon, A, Furnell, A, Mead, A, Levine, AP, Manzur, A, Thrasher, A, Greenhalgh, A, Parker, A, Sanchis-Juan, A, Richter, A, Gardham, A, Lawrie, A, Sohal, A, Creaser-Myers, A, Frary, A, Greinacher, A, Themistocleous, A, Peacock, AJ, Marshall, A, Mumford, A, Rice, A, Webster, A, Brady, A, Koziell, A, Manson, A, Chandra, A, Hensiek, A, Veld, AHIT, Maw, A, Kelly, AM, Moore, A, Vonk Noordegraaf, A, Attwood, A, Herwadkar, A, Ghofrani, A, Houweling, AC, Girerd, B, Furie, B, Treacy, CM, Millar, CM, Sewell, C, Roughley, C, Titterton, C, Williamson, C, Hadinnapola, C, Deshpande, C, Toh, CH, Bacchelli, C, Patch, C, Geet, CV, Babbs, C, Bryson, C, Penkett, CJ, Rhodes, CJ, Watt, C, Bethune, C, Booth, C, Lentaigne, C, McJannet, C, Church, C, French, C, Samarghitean, C, Halmagyi, C, Gale, D, Greene, D, Hart, D, Allsup, D, Bennett, D, Edgar, D, Kiely, DG, Gosal, D, Perry, DJ, Keeling, D, Montani, D, Shipley, D, Whitehorn, D, Fletcher, D, Krishnakumar, D, Grozeva, D, Kumararatne, D, Thompson, D, Josifova, D, Maher, E, Wong, EKS, Murphy, E, Dewhurst, E, Louka, E, Rosser, E, Chalmers, E, Colby, E, Drewe, E, McDermott, E, Thomas, E, Staples, E, Clement, E, Matthews, E, Wakeling, E, Oksenhendler, E, Turro, E, Reid, E, Wassmer, E, Raymond, FL, Hu, F, Kennedy, F, Soubrier, F, Flinter, F, Kovacs, G, Polwarth, G, Ambegaonkar, G, Arno, G, Hudson, G, Woods, G, Coghlan, G, Hayman, G, Arumugakani, G, Schotte, G, Cook, HT, Alachkar, H, Lango Allen, H, Lango-Allen, H, Stark, H, Stauss, H, Schulze, H, Boggard, HJ, Baxendale, H, Dolling, H, Firth, H, Gall, H, Watson, H, Longhurst, H, Markus, HS, Watkins, H, Simeoni, I, Emmerson, I, Roberts, I, Quinti, I, Wanjiku, I, Gibbs, JSR, Thaventhiran, J, Whitworth, J, Hurst, J, Collins, J, Suntharalingam, J, Payne, J, Thachil, J, Martin, JM, Carmichael, J, Maimaris, J, Paterson, J, Pepke-Zaba, J, Heemskerk, JWM, Gebhart, J, Davis, J, Pasi, J, Bradley, JR, Wharton, J, Stephens, J, Rankin, J, Anderson, J, Vogt, J, Von Ziegenweldt, J, Rehnstrom, K, Megy, K, Talks, K, Peerlinck, K, Yates, K, Freson, K, Stirrups, K, Gomez, K, Smith, KGC, Rue-Albrecht, K, Gilmour, K, Masati, L, Scelsi, L, Southgate, L, Ranganathan, L, Ginsberg, L, Devlin, L, Willcocks, L, Ormondroyd, L, Lorenzo, L, Harper, L, Allen, L, Daugherty, L, Chitre, M, Kurian, M, Humbert, M, Tischkowitz, M, Bitner-Glindzicz, M, Erwood, M, Scully, M, Veltman, M, Caulfield, M, Layton, M, McCarthy, M, Ponsford, M, Toshner, M, Bleda, M, Wilkins, M, Mathias, M, Reilly, M, Afzal, M, Brown, M, Rondina, M, Stubbs, M, Haimel, M, Lees, M, Laffan, MA, Browning, M, Gattens, M, Richards, M, Michaelides, M, Lambert, MP, Makris, M, De Vries, M, Mahdi-Rogers, M, Saleem, M, Thomas, M, Holder, M, Eyries, M, Clements-Brod, N, Canham, N, Dormand, N, Zuydam, NV, Kingston, N, Ghali, N, Cooper, N, Morrell, NW, Yeatman, N, Roy, N, Shamardina, O, Alavijeh, OS, Gresele, P, Nurden, P, Chinnery, P, Deegan, P, Yong, P, Man, PYW, Corris, PA, Calleja, P, Gissen, P, Bolton-Maggs, P, Rayner-Matthews, P, Ghataorhe, PK, Gordins, P, Stein, P, Collins, P, Dixon, P, Kelleher, P, Ancliff, P, Yu, P, Tait, RC, Linger, R, Doffinger, R, Machado, R, Kazmi, R, Sargur, R, Favier, R, Tan, R, Liesner, R, Antrobus, R, Sandford, R, Scott, R, Trembath, R, Horvath, R, Hadden, R, Mackenzieross, RV, Henderson, R, MacLaren, R, James, R, Ghurye, R, Dacosta, R, Hague, R, Mapeta, R, Armstrong, R, Noorani, S, Murng, S, Santra, S, Tuna, S, Johnson, S, Chong, S, Lear, S, Walker, S, Goddard, S, Mangles, S, Westbury, S, Mehta, S, Hackett, S, Nejentsev, S, Moledina, S, Bibi, S, Meehan, S, Othman, S, Revel-Vilk, S, Holden, S, McGowan, S, Staines, S, Savic, S, Burns, S, Grigoriadou, S, Papadia, S, Ashford, S, Schulman, S, Ali, S, Park, SM, Davies, S, Stock, S, Ali, S, Deevi, SVV, Gräf, S, Ghio, S, Wort, SJ, Jolles, S, Austin, S, Welch, S, Meacham, S, Rankin, S, Walker, S, Seneviratne, S, Holder, S, Sivapalaratnam, S, Richardson, S, Kuijpers, TW, Kuijpers, TW, Bariana, TK, Bakchoul, T, Everington, T, Renton, T, Young, T, Aitman, T, Warner, TQ, Vale, T, Hammerton, T, Pollock, V, Matser, V, Cookson, V, Clowes, V, Qasim, W, Wei, W, Erber, WN, Ouwehand, WH, Astle, W, Egner, W, Turek, W, Henskens, Y, Tan, Y & Lynch, AG 2018, 'Telomerecat: A ploidy-agnostic method for estimating telomere length from whole genome sequencing data' Scientific Reports, vol. 8, no. 1, 1300. https://doi.org/10.1038/s41598-017-14403-y

Telomerecat : A ploidy-agnostic method for estimating telomere length from whole genome sequencing data. / Farmery, James H.R.; Smith, Mike L.; Huissoon, Aarnoud; Furnell, Abigail; Mead, Adam; Levine, Adam P.; Manzur, Adnan; Thrasher, Adrian; Greenhalgh, Alan; Parker, Alasdair; Sanchis-Juan, Alba; Richter, Alex; Gardham, Alice; Lawrie, Allan; Sohal, Aman; Creaser-Myers, Amanda; Frary, Amy; Greinacher, Andreas; Themistocleous, Andreas; Peacock, Andrew J.; Marshall, Andrew; Mumford, Andrew; Rice, Andrew; Webster, Andrew; Brady, Angie; Koziell, Ania; Manson, Ania; Chandra, Anita; Hensiek, Anke; Veld, Anna Huis In T.; Maw, Anna; Kelly, Anne M.; Moore, Anthony; Vonk Noordegraaf, Anton; Attwood, Antony; Herwadkar, Archana; Ghofrani, Ardi; Houweling, Arjan C.; Girerd, Barbara; Furie, Bruce; Treacy, Carmen M.; Millar, Carolyn M.; Sewell, Carrock; Roughley, Catherine; Titterton, Catherine; Williamson, Catherine; Hadinnapola, Charaka; Deshpande, Charu; Toh, Cheng Hock; Bacchelli, Chiara; Patch, Chris; Geet, Chris Van; Babbs, Christian; Bryson, Christine; Penkett, Christopher J.; Rhodes, Christopher J.; Watt, Christopher; Bethune, Claire; Booth, Claire; Lentaigne, Claire; McJannet, Coleen; Church, Colin; French, Courtney; Samarghitean, Crina; Halmagyi, Csaba; Gale, Daniel; Greene, Daniel; Hart, Daniel; Allsup, David; Bennett, David; Edgar, David; Kiely, David G.; Gosal, David; Perry, David J.; Keeling, David; Montani, David; Shipley, Debbie; Whitehorn, Deborah; Fletcher, Debra; Krishnakumar, Deepa; Grozeva, Detelina; Kumararatne, Dinakantha; Thompson, Dorothy; Josifova, Dragana; Maher, Eamonn; Wong, Edwin K.S.; Murphy, Elaine; Dewhurst, Eleanor; Louka, Eleni; Rosser, Elisabeth; Chalmers, Elizabeth; Colby, Elizabeth; Drewe, Elizabeth; McDermott, Elizabeth; Thomas, Ellen; Staples, Emily; Clement, Emma; Matthews, Emma; Wakeling, Emma; Oksenhendler, Eric; Turro, Ernest; Reid, Evan; Wassmer, Evangeline; Raymond, F. Lucy; Hu, Fengyuan; Kennedy, Fiona; Soubrier, Florent; Flinter, Frances; Kovacs, Gabor; Polwarth, Gary; Ambegaonkar, Gautum; Arno, Gavin; Hudson, Gavin; Woods, Geoff; Coghlan, Gerry; Hayman, Grant; Arumugakani, Gururaj; Schotte, Gwen; Cook, H. Terry; Alachkar, Hana; Lango Allen, Hana; Lango-Allen, Hana; Stark, Hannah; Stauss, Hans; Schulze, Harald; Boggard, Harm J.; Baxendale, Helen; Dolling, Helen; Firth, Helen; Gall, Henning; Watson, Henry; Longhurst, Hilary; Markus, Hugh S.; Watkins, Hugh; Simeoni, Ilenia; Emmerson, Ingrid; Roberts, Irene; Quinti, Isabella; Wanjiku, Ivy; Gibbs, J. Simon R.; Thaventhiran, James; Whitworth, James; Hurst, Jane; Collins, Janine; Suntharalingam, Jay; Payne, Jeanette; Thachil, Jecko; Martin, Jennifer M.; Carmichael, Jenny; Maimaris, Jesmeen; Paterson, Joan; Pepke-Zaba, Joanna; Heemskerk, Johan W.M.; Gebhart, Johanna; Davis, John; Pasi, John; Bradley, John R.; Wharton, John; Stephens, Jonathan; Rankin, Julia; Anderson, Julie; Vogt, Julie; Von Ziegenweldt, Julie; Rehnstrom, Karola; Megy, Karyn; Talks, Kate; Peerlinck, Kathelijne; Yates, Katherine; Freson, Kathleen; Stirrups, Kathleen; Gomez, Keith; Smith, Kenneth G.C.; Rue-Albrecht, Kevin; Gilmour, Kimberley; Masati, Larahmie; Scelsi, Laura; Southgate, Laura; Ranganathan, Lavanya; Ginsberg, Lionel; Devlin, Lisa; Willcocks, Lisa; Ormondroyd, Liz; Lorenzo, Lorena; Harper, Lorraine; Allen, Louise; Daugherty, Louise; Chitre, Manali; Kurian, Manju; Humbert, Marc; Tischkowitz, Marc; Bitner-Glindzicz, Maria; Erwood, Marie; Scully, Marie; Veltman, Marijke; Caulfield, Mark; Layton, Mark; McCarthy, Mark; Ponsford, Mark; Toshner, Mark; Bleda, Marta; Wilkins, Martin; Mathias, Mary; Reilly, Mary; Afzal, Maryam; Brown, Matthew; Rondina, Matthew; Stubbs, Matthew; Haimel, Matthias; Lees, Melissa; Laffan, Michael A.; Browning, Michael; Gattens, Michael; Richards, Michael; Michaelides, Michel; Lambert, Michele P.; Makris, Mike; De Vries, Minka; Mahdi-Rogers, Mohamed; Saleem, Moin; Thomas, Moira; Holder, Muriel; Eyries, Mélanie; Clements-Brod, Naomi; Canham, Natalie; Dormand, Natalie; Zuydam, Natalie Van; Kingston, Nathalie; Ghali, Neeti; Cooper, Nichola; Morrell, Nicholas W.; Yeatman, Nigel; Roy, Noémi; Shamardina, Olga; Alavijeh, Omid S.; Gresele, Paolo; Nurden, Paquita; Chinnery, Patrick; Deegan, Patrick; Yong, Patrick; Man, Patrick Yu Wai; Corris, Paul A.; Calleja, Paul; Gissen, Paul; Bolton-Maggs, Paula; Rayner-Matthews, Paula; Ghataorhe, Pavandeep K.; Gordins, Pavel; Stein, Penelope; Collins, Peter; Dixon, Peter; Kelleher, Peter; Ancliff, Phil; Yu, Ping; Tait, R. Campbell; Linger, Rachel; Doffinger, Rainer; Machado, Rajiv; Kazmi, Rashid; Sargur, Ravishankar; Favier, Remi; Tan, Rhea; Liesner, Ri; Antrobus, Richard; Sandford, Richard; Scott, Richard; Trembath, Richard; Horvath, Rita; Hadden, Rob; Mackenzieross, Rob V.; Henderson, Robert; MacLaren, Robert; James, Roger; Ghurye, Rohit; Dacosta, Rosa; Hague, Rosie; Mapeta, Rutendo; Armstrong, Ruth; Noorani, Sadia; Murng, Sai; Santra, Saikat; Tuna, Salih; Johnson, Sally; Chong, Sam; Lear, Sara; Walker, Sara; Goddard, Sarah; Mangles, Sarah; Westbury, Sarah; Mehta, Sarju; Hackett, Scott; Nejentsev, Sergey; Moledina, Shahin; Bibi, Shahnaz; Meehan, Sharon; Othman, Shokri; Revel-Vilk, Shoshana; Holden, Simon; McGowan, Simon; Staines, Simon; Savic, Sinisa; Burns, Siobhan; Grigoriadou, Sofia; Papadia, Sofia; Ashford, Sofie; Schulman, Sol; Ali, Sonia; Park, Soo Mi; Davies, Sophie; Stock, Sophie; Ali, Souad; Deevi, Sri V.V.; Gräf, Stefan; Ghio, Stefano; Wort, Stephen J.; Jolles, Stephen; Austin, Steve; Welch, Steve; Meacham, Stuart; Rankin, Stuart; Walker, Suellen; Seneviratne, Suranjith; Holder, Susan; Sivapalaratnam, Suthesh; Richardson, Sylvia; Kuijpers, Taco W.; Kuijpers, Taco W.; Bariana, Tadbir K.; Bakchoul, Tamam; Everington, Tamara; Renton, Tara; Young, Tim; Aitman, Timothy; Warner, Timothy Q.; Vale, Tom; Hammerton, Tracey; Pollock, Val; Matser, Vera; Cookson, Victoria; Clowes, Virginia; Qasim, Waseem; Wei, Wei; Erber, Wendy N.; Ouwehand, Willem H.; Astle, William; Egner, William; Turek, Wojciech; Henskens, Yvonne; Tan, Yvonne; Lynch, Andy G.

In: Scientific Reports, Vol. 8, No. 1, 1300, 01.12.2018.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Telomerecat

T2 - A ploidy-agnostic method for estimating telomere length from whole genome sequencing data

AU - Farmery, James H.R.

AU - Smith, Mike L.

AU - Huissoon, Aarnoud

AU - Furnell, Abigail

AU - Mead, Adam

AU - Levine, Adam P.

AU - Manzur, Adnan

AU - Thrasher, Adrian

AU - Greenhalgh, Alan

AU - Parker, Alasdair

AU - Sanchis-Juan, Alba

AU - Richter, Alex

AU - Gardham, Alice

AU - Lawrie, Allan

AU - Sohal, Aman

AU - Creaser-Myers, Amanda

AU - Frary, Amy

AU - Greinacher, Andreas

AU - Themistocleous, Andreas

AU - Peacock, Andrew J.

AU - Marshall, Andrew

AU - Mumford, Andrew

AU - Rice, Andrew

AU - Webster, Andrew

AU - Brady, Angie

AU - Koziell, Ania

AU - Manson, Ania

AU - Chandra, Anita

AU - Hensiek, Anke

AU - Veld, Anna Huis In T.

AU - Maw, Anna

AU - Kelly, Anne M.

AU - Moore, Anthony

AU - Vonk Noordegraaf, Anton

AU - Attwood, Antony

AU - Herwadkar, Archana

AU - Ghofrani, Ardi

AU - Houweling, Arjan C.

AU - Girerd, Barbara

AU - Furie, Bruce

AU - Treacy, Carmen M.

AU - Millar, Carolyn M.

AU - Sewell, Carrock

AU - Roughley, Catherine

AU - Titterton, Catherine

AU - Williamson, Catherine

AU - Hadinnapola, Charaka

AU - Deshpande, Charu

AU - Toh, Cheng Hock

AU - Bacchelli, Chiara

AU - Patch, Chris

AU - Geet, Chris Van

AU - Babbs, Christian

AU - Bryson, Christine

AU - Penkett, Christopher J.

AU - Rhodes, Christopher J.

AU - Watt, Christopher

AU - Bethune, Claire

AU - Booth, Claire

AU - Lentaigne, Claire

AU - McJannet, Coleen

AU - Church, Colin

AU - French, Courtney

AU - Samarghitean, Crina

AU - Halmagyi, Csaba

AU - Gale, Daniel

AU - Greene, Daniel

AU - Hart, Daniel

AU - Allsup, David

AU - Bennett, David

AU - Edgar, David

AU - Kiely, David G.

AU - Gosal, David

AU - Perry, David J.

AU - Keeling, David

AU - Montani, David

AU - Shipley, Debbie

AU - Whitehorn, Deborah

AU - Fletcher, Debra

AU - Krishnakumar, Deepa

AU - Grozeva, Detelina

AU - Kumararatne, Dinakantha

AU - Thompson, Dorothy

AU - Josifova, Dragana

AU - Maher, Eamonn

AU - Wong, Edwin K.S.

AU - Murphy, Elaine

AU - Dewhurst, Eleanor

AU - Louka, Eleni

AU - Rosser, Elisabeth

AU - Chalmers, Elizabeth

AU - Colby, Elizabeth

AU - Drewe, Elizabeth

AU - McDermott, Elizabeth

AU - Thomas, Ellen

AU - Staples, Emily

AU - Clement, Emma

AU - Matthews, Emma

AU - Wakeling, Emma

AU - Oksenhendler, Eric

AU - Turro, Ernest

AU - Reid, Evan

AU - Wassmer, Evangeline

AU - Raymond, F. Lucy

AU - Hu, Fengyuan

AU - Kennedy, Fiona

AU - Soubrier, Florent

AU - Flinter, Frances

AU - Kovacs, Gabor

AU - Polwarth, Gary

AU - Ambegaonkar, Gautum

AU - Arno, Gavin

AU - Hudson, Gavin

AU - Woods, Geoff

AU - Coghlan, Gerry

AU - Hayman, Grant

AU - Arumugakani, Gururaj

AU - Schotte, Gwen

AU - Cook, H. Terry

AU - Alachkar, Hana

AU - Lango Allen, Hana

AU - Lango-Allen, Hana

AU - Stark, Hannah

AU - Stauss, Hans

AU - Schulze, Harald

AU - Boggard, Harm J.

AU - Baxendale, Helen

AU - Dolling, Helen

AU - Firth, Helen

AU - Gall, Henning

AU - Watson, Henry

AU - Longhurst, Hilary

AU - Markus, Hugh S.

AU - Watkins, Hugh

AU - Simeoni, Ilenia

AU - Emmerson, Ingrid

AU - Roberts, Irene

AU - Quinti, Isabella

AU - Wanjiku, Ivy

AU - Gibbs, J. Simon R.

AU - Thaventhiran, James

AU - Whitworth, James

AU - Hurst, Jane

AU - Collins, Janine

AU - Suntharalingam, Jay

AU - Payne, Jeanette

AU - Thachil, Jecko

AU - Martin, Jennifer M.

AU - Carmichael, Jenny

AU - Maimaris, Jesmeen

AU - Paterson, Joan

AU - Pepke-Zaba, Joanna

AU - Heemskerk, Johan W.M.

AU - Gebhart, Johanna

AU - Davis, John

AU - Pasi, John

AU - Bradley, John R.

AU - Wharton, John

AU - Stephens, Jonathan

AU - Rankin, Julia

AU - Anderson, Julie

AU - Vogt, Julie

AU - Von Ziegenweldt, Julie

AU - Rehnstrom, Karola

AU - Megy, Karyn

AU - Talks, Kate

AU - Peerlinck, Kathelijne

AU - Yates, Katherine

AU - Freson, Kathleen

AU - Stirrups, Kathleen

AU - Gomez, Keith

AU - Smith, Kenneth G.C.

AU - Rue-Albrecht, Kevin

AU - Gilmour, Kimberley

AU - Masati, Larahmie

AU - Scelsi, Laura

AU - Southgate, Laura

AU - Ranganathan, Lavanya

AU - Ginsberg, Lionel

AU - Devlin, Lisa

AU - Willcocks, Lisa

AU - Ormondroyd, Liz

AU - Lorenzo, Lorena

AU - Harper, Lorraine

AU - Allen, Louise

AU - Daugherty, Louise

AU - Chitre, Manali

AU - Kurian, Manju

AU - Humbert, Marc

AU - Tischkowitz, Marc

AU - Bitner-Glindzicz, Maria

AU - Erwood, Marie

AU - Scully, Marie

AU - Veltman, Marijke

AU - Caulfield, Mark

AU - Layton, Mark

AU - McCarthy, Mark

AU - Ponsford, Mark

AU - Toshner, Mark

AU - Bleda, Marta

AU - Wilkins, Martin

AU - Mathias, Mary

AU - Reilly, Mary

AU - Afzal, Maryam

AU - Brown, Matthew

AU - Rondina, Matthew

AU - Stubbs, Matthew

AU - Haimel, Matthias

AU - Lees, Melissa

AU - Laffan, Michael A.

AU - Browning, Michael

AU - Gattens, Michael

AU - Richards, Michael

AU - Michaelides, Michel

AU - Lambert, Michele P.

AU - Makris, Mike

AU - De Vries, Minka

AU - Mahdi-Rogers, Mohamed

AU - Saleem, Moin

AU - Thomas, Moira

AU - Holder, Muriel

AU - Eyries, Mélanie

AU - Clements-Brod, Naomi

AU - Canham, Natalie

AU - Dormand, Natalie

AU - Zuydam, Natalie Van

AU - Kingston, Nathalie

AU - Ghali, Neeti

AU - Cooper, Nichola

AU - Morrell, Nicholas W.

AU - Yeatman, Nigel

AU - Roy, Noémi

AU - Shamardina, Olga

AU - Alavijeh, Omid S.

AU - Gresele, Paolo

AU - Nurden, Paquita

AU - Chinnery, Patrick

AU - Deegan, Patrick

AU - Yong, Patrick

AU - Man, Patrick Yu Wai

AU - Corris, Paul A.

AU - Calleja, Paul

AU - Gissen, Paul

AU - Bolton-Maggs, Paula

AU - Rayner-Matthews, Paula

AU - Ghataorhe, Pavandeep K.

AU - Gordins, Pavel

AU - Stein, Penelope

AU - Collins, Peter

AU - Dixon, Peter

AU - Kelleher, Peter

AU - Ancliff, Phil

AU - Yu, Ping

AU - Tait, R. Campbell

AU - Linger, Rachel

AU - Doffinger, Rainer

AU - Machado, Rajiv

AU - Kazmi, Rashid

AU - Sargur, Ravishankar

AU - Favier, Remi

AU - Tan, Rhea

AU - Liesner, Ri

AU - Antrobus, Richard

AU - Sandford, Richard

AU - Scott, Richard

AU - Trembath, Richard

AU - Horvath, Rita

AU - Hadden, Rob

AU - Mackenzieross, Rob V.

AU - Henderson, Robert

AU - MacLaren, Robert

AU - James, Roger

AU - Ghurye, Rohit

AU - Dacosta, Rosa

AU - Hague, Rosie

AU - Mapeta, Rutendo

AU - Armstrong, Ruth

AU - Noorani, Sadia

AU - Murng, Sai

AU - Santra, Saikat

AU - Tuna, Salih

AU - Johnson, Sally

AU - Chong, Sam

AU - Lear, Sara

AU - Walker, Sara

AU - Goddard, Sarah

AU - Mangles, Sarah

AU - Westbury, Sarah

AU - Mehta, Sarju

AU - Hackett, Scott

AU - Nejentsev, Sergey

AU - Moledina, Shahin

AU - Bibi, Shahnaz

AU - Meehan, Sharon

AU - Othman, Shokri

AU - Revel-Vilk, Shoshana

AU - Holden, Simon

AU - McGowan, Simon

AU - Staines, Simon

AU - Savic, Sinisa

AU - Burns, Siobhan

AU - Grigoriadou, Sofia

AU - Papadia, Sofia

AU - Ashford, Sofie

AU - Schulman, Sol

AU - Ali, Sonia

AU - Park, Soo Mi

AU - Davies, Sophie

AU - Stock, Sophie

AU - Ali, Souad

AU - Deevi, Sri V.V.

AU - Gräf, Stefan

AU - Ghio, Stefano

AU - Wort, Stephen J.

AU - Jolles, Stephen

AU - Austin, Steve

AU - Welch, Steve

AU - Meacham, Stuart

AU - Rankin, Stuart

AU - Walker, Suellen

AU - Seneviratne, Suranjith

AU - Holder, Susan

AU - Sivapalaratnam, Suthesh

AU - Richardson, Sylvia

AU - Kuijpers, Taco W.

AU - Kuijpers, Taco W.

AU - Bariana, Tadbir K.

AU - Bakchoul, Tamam

AU - Everington, Tamara

AU - Renton, Tara

AU - Young, Tim

AU - Aitman, Timothy

AU - Warner, Timothy Q.

AU - Vale, Tom

AU - Hammerton, Tracey

AU - Pollock, Val

AU - Matser, Vera

AU - Cookson, Victoria

AU - Clowes, Virginia

AU - Qasim, Waseem

AU - Wei, Wei

AU - Erber, Wendy N.

AU - Ouwehand, Willem H.

AU - Astle, William

AU - Egner, William

AU - Turek, Wojciech

AU - Henskens, Yvonne

AU - Tan, Yvonne

AU - Lynch, Andy G.

PY - 2018/12/1

Y1 - 2018/12/1

N2 - Telomere length is a risk factor in disease and the dynamics of telomere length are crucial to our understanding of cell replication and vitality. The proliferation of whole genome sequencing represents an unprecedented opportunity to glean new insights into telomere biology on a previously unimaginable scale. To this end, a number of approaches for estimating telomere length from whole-genome sequencing data have been proposed. Here we present Telomerecat, a novel approach to the estimation of telomere length. Previous methods have been dependent on the number of telomeres present in a cell being known, which may be problematic when analysing aneuploid cancer data and non-human samples. Telomerecat is designed to be agnostic to the number of telomeres present, making it suited for the purpose of estimating telomere length in cancer studies. Telomerecat also accounts for interstitial telomeric reads and presents a novel approach to dealing with sequencing errors. We show that Telomerecat performs well at telomere length estimation when compared to leading experimental and computational methods. Furthermore, we show that it detects expected patterns in longitudinal data, repeated measurements, and cross-species comparisons. We also apply the method to a cancer cell data, uncovering an interesting relationship with the underlying telomerase genotype.

AB - Telomere length is a risk factor in disease and the dynamics of telomere length are crucial to our understanding of cell replication and vitality. The proliferation of whole genome sequencing represents an unprecedented opportunity to glean new insights into telomere biology on a previously unimaginable scale. To this end, a number of approaches for estimating telomere length from whole-genome sequencing data have been proposed. Here we present Telomerecat, a novel approach to the estimation of telomere length. Previous methods have been dependent on the number of telomeres present in a cell being known, which may be problematic when analysing aneuploid cancer data and non-human samples. Telomerecat is designed to be agnostic to the number of telomeres present, making it suited for the purpose of estimating telomere length in cancer studies. Telomerecat also accounts for interstitial telomeric reads and presents a novel approach to dealing with sequencing errors. We show that Telomerecat performs well at telomere length estimation when compared to leading experimental and computational methods. Furthermore, we show that it detects expected patterns in longitudinal data, repeated measurements, and cross-species comparisons. We also apply the method to a cancer cell data, uncovering an interesting relationship with the underlying telomerase genotype.

UR - http://www.scopus.com/inward/record.url?scp=85040985054&partnerID=8YFLogxK

U2 - 10.1038/s41598-017-14403-y

DO - 10.1038/s41598-017-14403-y

M3 - Article

VL - 8

JO - Scientific Reports

JF - Scientific Reports

SN - 2045-2322

IS - 1

M1 - 1300

ER -