Techniques for paraspeckle visualisation and analysis in cellular stress and disease contexts

Finn McCluggage

doi:10.26182/qhkm-6m87

Techniques for paraspeckle visualisation and analysis in cellular stress and disease contexts

Finn McCluggage

Research output: Thesis › Doctoral Thesis

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Abstract

Paraspeckles are subnuclear bodies formed via interactions between the long non-coding RNANEAT1_2 and paraspeckle associated proteins. Increased formation of paraspeckles is a common feature between a wide range of cellular stress responses. In this thesis, I aimed to explore and utilize novel methodologies for paraspeckle visualisation in cellular stress and disease contexts. I first explored the use of FLIM-FRET microscopy to develop a paraspeckle biosensor. Next, I investigated the ability of a large panel of Australian metabolites to moderate paraspeckles. Finally, using live-cell imaging techniques I investigated paraspeckle dynamics throughout confined migration through microchannels.

Original language	English
Qualification	Doctor of Philosophy
Awarding Institution	The University of Western Australia
Supervisors/Advisors	Fox, Archa, Supervisor Choi, Yu Suk, Supervisor Smith, Nicole, Supervisor
Thesis sponsors	Australian Government Research Training Program
Award date	14 Jun 2024
DOIs	https://doi.org/10.26182/qhkm-6m87
Publication status	Unpublished - 2023

Embargo information

Paraspeckles
long non-coding RNA
FLIM-FRET
small molecules
metastasis
CRISPR

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10.26182/qhkm-6m87

THESIS - DOCTOR OF PHILOSOPHY - MCCLUGGAGE Finn Allan - 2023
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2 Article
1 Review article

Confined environments induce polarized paraspeckle condensates
Todorovski, V., McCluggage, F., Li, Y., Meid, A., Spatz, J. P., Holle, A. W., Fox, A. H. & Choi, Y. S., 3 Feb 2023, In: Communications Biology. 6, 1, 145.
Research output: Contribution to journal › Article › peer-review

Open Access
11 Citations (Scopus)
Tumour mutations in long noncoding RNAs enhance cell fitness
Esposito, R., Lanzós, A., Uroda, T., Ramnarayanan, S., Büchi, I., Polidori, T., Guillen-Ramirez, H., Mihaljevic, A., Merlin, B. M., Mela, L., Zoni, E., Hovhannisyan, L., McCluggage, F., Medo, M., Basile, G., Meise, D. F., Zwyssig, S., Wenger, C., Schwarz, K. & Vancura, A. & 12 others, Bosch-Guiteras, N., Andrades, Á., Tham, A. M., Roemmele, M., Ochsenbein, A. F., Riether, C., Kruithof-de Julio, M., Zimmer, Y., Medová, M., Stroka, D., Fox, A. & Johnson, R., Dec 2023, In: Nature Communications. 14, 1, 3342.
Research output: Contribution to journal › Article › peer-review

Open Access
7 Citations (Scopus)
Paraspeckle nuclear condensates: Global sensors of cell stress?
McCluggage, F. & Fox, A. H., May 2021, In: BioEssays. 43, 5, 2000245.
Research output: Contribution to journal › Review article › peer-review
56 Citations (Scopus)

Cite this

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AB - Paraspeckles are subnuclear bodies formed via interactions between the long non-coding RNANEAT1_2 and paraspeckle associated proteins. Increased formation of paraspeckles is a common feature between a wide range of cellular stress responses. In this thesis, I aimed to explore and utilize novel methodologies for paraspeckle visualisation in cellular stress and disease contexts. I first explored the use of FLIM-FRET microscopy to develop a paraspeckle biosensor. Next, I investigated the ability of a large panel of Australian metabolites to moderate paraspeckles. Finally, using live-cell imaging techniques I investigated paraspeckle dynamics throughout confined migration through microchannels.

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DO - 10.26182/qhkm-6m87

M3 - Doctoral Thesis

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Techniques for paraspeckle visualisation and analysis in cellular stress and disease contexts

Abstract

Embargo information

Access to Document

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Research output

Confined environments induce polarized paraspeckle condensates

Tumour mutations in long noncoding RNAs enhance cell fitness

Paraspeckle nuclear condensates: Global sensors of cell stress?

Cite this