Targeting Glycoside Hydrolases and Central Carbon Metabolism to Disrupt Burkholderia pseudomallei Pathogenesis

Clare Moran

doi:10.26182/6abx-0k61

Targeting Glycoside Hydrolases and Central Carbon Metabolism to Disrupt Burkholderia pseudomallei Pathogenesis

Clare Moran

School of Biomedical Sciences

Research output: Thesis › Doctoral Thesis

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Abstract

The processes of carbohydrate metabolism are important for virulence in many heterotrophic bacterial pathogens. Carbohydrate-active enzymes (CAZymes) such as glycoside hydrolases (GHs) may therefore provide novel antimicrobial targets. Burkholderia pseudomallei is the causative agent of melioidosis, a disease characterized by severe pneumonia and septicaemia. Despite B.pseudomallei encoding for 35 GHs, only a few have been characterized. The aim of this study was to investigate the role of four putative GHs encoded by the genes bpsl0500, bpss0671, bpsl0611, bpss0159 and a Class II fructose-1,6-bisphosphate aldolase, encoded by bpsl0798, by the generation and characterisation of mutant strains.

Original language	English
Qualification	Doctor of Philosophy
Awarding Institution	The University of Western Australia
Supervisors/Advisors	Sarkar-Tyson, Mitali, Supervisor Debowski, Aleksandra, Supervisor Stubbs, Keith, Supervisor Titball, Richard W., Supervisor, External person
Thesis sponsors	Australian Government Research Training Program
Award date	29 May 2023
DOIs	https://doi.org/10.26182/6abx-0k61
Publication status	Unpublished - 2023

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10.26182/6abx-0k61

THESIS - DOCTOR OF PHILOSOPHY - MORAN Clare - 2023
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Cite this

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title = "Targeting Glycoside Hydrolases and Central Carbon Metabolism to Disrupt Burkholderia pseudomallei Pathogenesis",

abstract = "The processes of carbohydrate metabolism are important for virulence in many heterotrophic bacterial pathogens. Carbohydrate-active enzymes (CAZymes) such as glycoside hydrolases (GHs) may therefore provide novel antimicrobial targets. Burkholderia pseudomallei is the causative agent of melioidosis, a disease characterized by severe pneumonia and septicaemia. Despite B.pseudomallei encoding for 35 GHs, only a few have been characterized. The aim of this study was to investigate the role of four putative GHs encoded by the genes bpsl0500, bpss0671, bpsl0611, bpss0159 and a Class II fructose-1,6-bisphosphate aldolase, encoded by bpsl0798, by the generation and characterisation of mutant strains.",

keywords = "carbohydrate metabolism, Burkholderia pseudomallei, CAZyme, glycoside hydrolase, pathogenesis",

author = "Clare Moran",

year = "2023",

doi = "10.26182/6abx-0k61",

language = "English",

school = "The University of Western Australia",

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TY - BOOK

T1 - Targeting Glycoside Hydrolases and Central Carbon Metabolism to Disrupt Burkholderia pseudomallei Pathogenesis

AU - Moran, Clare

PY - 2023

Y1 - 2023

N2 - The processes of carbohydrate metabolism are important for virulence in many heterotrophic bacterial pathogens. Carbohydrate-active enzymes (CAZymes) such as glycoside hydrolases (GHs) may therefore provide novel antimicrobial targets. Burkholderia pseudomallei is the causative agent of melioidosis, a disease characterized by severe pneumonia and septicaemia. Despite B.pseudomallei encoding for 35 GHs, only a few have been characterized. The aim of this study was to investigate the role of four putative GHs encoded by the genes bpsl0500, bpss0671, bpsl0611, bpss0159 and a Class II fructose-1,6-bisphosphate aldolase, encoded by bpsl0798, by the generation and characterisation of mutant strains.

AB - The processes of carbohydrate metabolism are important for virulence in many heterotrophic bacterial pathogens. Carbohydrate-active enzymes (CAZymes) such as glycoside hydrolases (GHs) may therefore provide novel antimicrobial targets. Burkholderia pseudomallei is the causative agent of melioidosis, a disease characterized by severe pneumonia and septicaemia. Despite B.pseudomallei encoding for 35 GHs, only a few have been characterized. The aim of this study was to investigate the role of four putative GHs encoded by the genes bpsl0500, bpss0671, bpsl0611, bpss0159 and a Class II fructose-1,6-bisphosphate aldolase, encoded by bpsl0798, by the generation and characterisation of mutant strains.

KW - carbohydrate metabolism

KW - Burkholderia pseudomallei

KW - CAZyme

KW - glycoside hydrolase

KW - pathogenesis

U2 - 10.26182/6abx-0k61

DO - 10.26182/6abx-0k61

M3 - Doctoral Thesis

ER -

Targeting Glycoside Hydrolases and Central Carbon Metabolism to Disrupt Burkholderia pseudomallei Pathogenesis

Abstract

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