Targeted manipulation of the mammalian epigenome

Eng Kiat Dennis Tan

doi:10.4225/23/5a978f31a2dcd

Targeted manipulation of the mammalian epigenome

Eng Kiat Dennis Tan

School of Molecular Sciences

Research output: Thesis › Doctoral Thesis

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Abstract

The ability to alter DNA methylation states at specific CG sites is instrumental for understanding the functional role of methylcytosine in the genome. This thesis research has demonstrated that the Epi-Cas9-SunTag is an excellent molecular tool that can induce highly specific and controllable editing of DNA methylation states at region(s) of interest. However, there remains multiple challenges to be resolved and is thoroughly discussed in this thesis. In conclusion, Epi-Cas9-SunTag is a powerful molecular tool for dissecting the functional roles of DNA methylation in the genome with the potential of being a highly specific approach for future targeted epigenetic therapies.

Original language	English
Qualification	Doctor of Philosophy
Awarding Institution	The University of Western Australia
Supervisors/Advisors	Lister, Ryan, Supervisor Millar, Harvey, Supervisor Ford, Ethan, Supervisor Small, Ian, Supervisor
Award date	5 Feb 2018
DOIs	https://doi.org/10.4225/23/5a978f31a2dcd
Publication status	Unpublished - 2018

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10.4225/23/5a978f31a2dcd

THESIS - DOCTOR OF PHILOSOPHY - TAN Dennis Eng Kiat - 2018
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title = "Targeted manipulation of the mammalian epigenome",

abstract = "The ability to alter DNA methylation states at specific CG sites is instrumental for understanding the functional role of methylcytosine in the genome. This thesis research has demonstrated that the Epi-Cas9-SunTag is an excellent molecular tool that can induce highly specific and controllable editing of DNA methylation states at region(s) of interest. However, there remains multiple challenges to be resolved and is thoroughly discussed in this thesis. In conclusion, Epi-Cas9-SunTag is a powerful molecular tool for dissecting the functional roles of DNA methylation in the genome with the potential of being a highly specific approach for future targeted epigenetic therapies. ",

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AB - The ability to alter DNA methylation states at specific CG sites is instrumental for understanding the functional role of methylcytosine in the genome. This thesis research has demonstrated that the Epi-Cas9-SunTag is an excellent molecular tool that can induce highly specific and controllable editing of DNA methylation states at region(s) of interest. However, there remains multiple challenges to be resolved and is thoroughly discussed in this thesis. In conclusion, Epi-Cas9-SunTag is a powerful molecular tool for dissecting the functional roles of DNA methylation in the genome with the potential of being a highly specific approach for future targeted epigenetic therapies.

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DO - 10.4225/23/5a978f31a2dcd

M3 - Doctoral Thesis

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Targeted manipulation of the mammalian epigenome

Abstract

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