Targeted manipulation of the mammalian epigenome

Eng Kiat Dennis Tan

Research output: ThesisDoctoral Thesis

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Abstract

The ability to alter DNA methylation states at specific CG sites is instrumental for understanding the functional role of methyl­cytosine in the genome. This thesis research has demonstrated that the Epi-Cas9-SunTag is an excellent molecular tool that can induce highly specific and controllable editing of DNA methylation states at region(s) of interest. However, there remains multiple challenges to be resolved and is thoroughly discussed in this thesis. In conclusion, Epi-Cas9-SunTag is a powerful molecular tool for dissecting the functional roles of DNA methylation in the genome with the potential of being a highly specific approach for future targeted epigenetic therapies.
Original languageEnglish
QualificationDoctor of Philosophy
Awarding Institution
  • The University of Western Australia
Supervisors/Advisors
  • Lister, Ryan, Supervisor
  • Millar, Harvey, Supervisor
  • Ford, Ethan, Supervisor
  • Small, Ian, Supervisor
Award date5 Feb 2018
DOIs
Publication statusUnpublished - 2018

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