Targeted delivery of an antisense oligonucleotide in the retina: uptake, distribution, stability, and effect

Elizabeth Rakoczy, Chooi-May Lai, Mark Watson, U. Seydel, Ian Constable

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26 Citations (Scopus)

Abstract

In this article, we describe the preliminary results of the development of an animal model that will enable us to study the effect of photoreceptor-derived debris accumulation on the normal function of the retina in vivo. An antisense oligonucleotide (Cat 5), saline, and two control oligonucleotides were injected into the vitreous of 7-week-old RCS-rdy+rats. The uptake, distribution, and persistence of the antisense oligonucleotide in the retina was demonstrated by fluorescent confocal microscopy, and the stability of the oligonucleotide was shown by GeneScan analysis using a fluorescein-labeled derivative of Cat 5 (Cat 5F). The accumulation of photoreceptor-derived debris was monitored by the number of undigested phagosomes in the RPE layer by light microscopy, Following intravitreal injection of Cat 5F, penetration of the oligonucleotide was observed in the ganglion cell layer in 2 hours and in the photoreceptor and pigment epithelial layers 3 days later, However, at 7, 28, and 56 days postinjection, only the RPE layer had significant amounts of Cat 5F present, Using GeneScan analysis, it was demonstrated that the fluorescein-labeled oligonucleotide present in the RPE layer was not degraded and it retained its original 19-mer length. There was no statistically significant difference in the number of phagosomes found in the RPE layer of control uninjected, saline-injected, and two sense and two antisense oligonucleotides-injected animals at 7 and 28 days postinjection, In contrast, the number of phagosomes was significantly higher (p
Original languageEnglish
Pages (from-to)207-213
JournalAntisense and Nucleic Acid Drug Development
Volume6
DOIs
Publication statusPublished - 1996

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