T cell cytokine responses to stimulation with Ureaplasma parvum in pregnancy

Peter Friedland; Tracey F. Lee-Pullen; Liz A. Nathan; Rory Watts; Jeffrey A. Keelan; Matt S. Payne; Demelza J. Ireland

doi:10.1016/j.jri.2016.03.002

T cell cytokine responses to stimulation with Ureaplasma parvum in pregnancy

Peter Friedland, Tracey F. Lee-Pullen, Liz A. Nathan, Rory Watts, Jeffrey A. Keelan, Matt S. Payne, Demelza J. Ireland

Research output: Contribution to journal › Article › peer-review

5 Citations (Scopus)

Abstract

© 2016 Elsevier Ireland Ltd.Ureaplasma spp. are a common vaginal microorganism causally linked to inflammation-driven preterm birth (PTB). The nature of the immune response to Ureaplasma spp. may influence PTB risk. This study sought to define maternal T cell cytokine responses to in vitro stimulation with Ureaplasma parvum serovar 3 (UpSV3) in vaginally colonised (UP+) and non-colonised (UP-) pregnant women. Whole blood flow cytometry demonstrated an increase (p = 0.027) in the baseline frequency of IFN?-positive CD3+CD4-(CD8+) T cells in UP+ women. UpSV3 stimulation resulted in a significant and specific increase (p = 0.001) in the frequency of IFN?-positive CD3+CD4-(CD8+) T cells, regardless of vaginal colonisation status. UpSV3 stimulation also increased the frequency of IFN?-positive CD3+CD4+ T cells, particularly in the UP+ group (p = 0.003). This is the first published study to examine T cell responses to Ureaplasma spp. exposure. Future appropriately-powered studies are needed to assess whether insufficient priming or a loss of tolerance to Ureaplasma spp. is occurring in UP+ women at risk of PTB.

Original language	English
Pages (from-to)	93-97
Number of pages	5
Journal	Journal of Reproductive Immunology
Volume	116
DOIs	https://doi.org/10.1016/j.jri.2016.03.002
Publication status	Published - 1 Aug 2016

Access to Document

10.1016/j.jri.2016.03.002

Cite this

@article{4a0d88c75efe41ac9cdf9f2dd305b051,

title = "T cell cytokine responses to stimulation with Ureaplasma parvum in pregnancy",

abstract = "{\textcopyright} 2016 Elsevier Ireland Ltd.Ureaplasma spp. are a common vaginal microorganism causally linked to inflammation-driven preterm birth (PTB). The nature of the immune response to Ureaplasma spp. may influence PTB risk. This study sought to define maternal T cell cytokine responses to in vitro stimulation with Ureaplasma parvum serovar 3 (UpSV3) in vaginally colonised (UP+) and non-colonised (UP-) pregnant women. Whole blood flow cytometry demonstrated an increase (p = 0.027) in the baseline frequency of IFN?-positive CD3+CD4-(CD8+) T cells in UP+ women. UpSV3 stimulation resulted in a significant and specific increase (p = 0.001) in the frequency of IFN?-positive CD3+CD4-(CD8+) T cells, regardless of vaginal colonisation status. UpSV3 stimulation also increased the frequency of IFN?-positive CD3+CD4+ T cells, particularly in the UP+ group (p = 0.003). This is the first published study to examine T cell responses to Ureaplasma spp. exposure. Future appropriately-powered studies are needed to assess whether insufficient priming or a loss of tolerance to Ureaplasma spp. is occurring in UP+ women at risk of PTB.",

author = "Peter Friedland and Lee-Pullen, {Tracey F.} and Nathan, {Liz A.} and Rory Watts and Keelan, {Jeffrey A.} and Payne, {Matt S.} and Ireland, {Demelza J.}",

year = "2016",

month = aug,

day = "1",

doi = "10.1016/j.jri.2016.03.002",

language = "English",

volume = "116",

pages = "93--97",

journal = "Journal of Reproductive Immunology",

issn = "0165-0378",

publisher = "Elsevier",

}

TY - JOUR

T1 - T cell cytokine responses to stimulation with Ureaplasma parvum in pregnancy

AU - Friedland, Peter

AU - Lee-Pullen, Tracey F.

AU - Nathan, Liz A.

AU - Watts, Rory

AU - Keelan, Jeffrey A.

AU - Payne, Matt S.

AU - Ireland, Demelza J.

PY - 2016/8/1

Y1 - 2016/8/1

N2 - © 2016 Elsevier Ireland Ltd.Ureaplasma spp. are a common vaginal microorganism causally linked to inflammation-driven preterm birth (PTB). The nature of the immune response to Ureaplasma spp. may influence PTB risk. This study sought to define maternal T cell cytokine responses to in vitro stimulation with Ureaplasma parvum serovar 3 (UpSV3) in vaginally colonised (UP+) and non-colonised (UP-) pregnant women. Whole blood flow cytometry demonstrated an increase (p = 0.027) in the baseline frequency of IFN?-positive CD3+CD4-(CD8+) T cells in UP+ women. UpSV3 stimulation resulted in a significant and specific increase (p = 0.001) in the frequency of IFN?-positive CD3+CD4-(CD8+) T cells, regardless of vaginal colonisation status. UpSV3 stimulation also increased the frequency of IFN?-positive CD3+CD4+ T cells, particularly in the UP+ group (p = 0.003). This is the first published study to examine T cell responses to Ureaplasma spp. exposure. Future appropriately-powered studies are needed to assess whether insufficient priming or a loss of tolerance to Ureaplasma spp. is occurring in UP+ women at risk of PTB.

AB - © 2016 Elsevier Ireland Ltd.Ureaplasma spp. are a common vaginal microorganism causally linked to inflammation-driven preterm birth (PTB). The nature of the immune response to Ureaplasma spp. may influence PTB risk. This study sought to define maternal T cell cytokine responses to in vitro stimulation with Ureaplasma parvum serovar 3 (UpSV3) in vaginally colonised (UP+) and non-colonised (UP-) pregnant women. Whole blood flow cytometry demonstrated an increase (p = 0.027) in the baseline frequency of IFN?-positive CD3+CD4-(CD8+) T cells in UP+ women. UpSV3 stimulation resulted in a significant and specific increase (p = 0.001) in the frequency of IFN?-positive CD3+CD4-(CD8+) T cells, regardless of vaginal colonisation status. UpSV3 stimulation also increased the frequency of IFN?-positive CD3+CD4+ T cells, particularly in the UP+ group (p = 0.003). This is the first published study to examine T cell responses to Ureaplasma spp. exposure. Future appropriately-powered studies are needed to assess whether insufficient priming or a loss of tolerance to Ureaplasma spp. is occurring in UP+ women at risk of PTB.

U2 - 10.1016/j.jri.2016.03.002

DO - 10.1016/j.jri.2016.03.002

M3 - Article

C2 - 27281123

SN - 0165-0378

VL - 116

SP - 93

EP - 97

JO - Journal of Reproductive Immunology

JF - Journal of Reproductive Immunology

ER -

T cell cytokine responses to stimulation with Ureaplasma parvum in pregnancy

Abstract

Access to Document

Fingerprint

Cite this