T cell cytokine responses to stimulation with Ureaplasma parvum in pregnancy

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    Abstract

    © 2016 Elsevier Ireland Ltd.Ureaplasma spp. are a common vaginal microorganism causally linked to inflammation-driven preterm birth (PTB). The nature of the immune response to Ureaplasma spp. may influence PTB risk. This study sought to define maternal T cell cytokine responses to in vitro stimulation with Ureaplasma parvum serovar 3 (UpSV3) in vaginally colonised (UP+) and non-colonised (UP-) pregnant women. Whole blood flow cytometry demonstrated an increase (p = 0.027) in the baseline frequency of IFN?-positive CD3+CD4-(CD8+) T cells in UP+ women. UpSV3 stimulation resulted in a significant and specific increase (p = 0.001) in the frequency of IFN?-positive CD3+CD4-(CD8+) T cells, regardless of vaginal colonisation status. UpSV3 stimulation also increased the frequency of IFN?-positive CD3+CD4+ T cells, particularly in the UP+ group (p = 0.003). This is the first published study to examine T cell responses to Ureaplasma spp. exposure. Future appropriately-powered studies are needed to assess whether insufficient priming or a loss of tolerance to Ureaplasma spp. is occurring in UP+ women at risk of PTB.
    Original languageEnglish
    Pages (from-to)93-97
    Number of pages5
    JournalJournal of Reproductive Immunology
    Volume116
    DOIs
    Publication statusPublished - 2016

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    Ureaplasma
    Cytokines
    T-Lymphocytes
    Pregnancy
    Premature Birth
    Ireland
    Pregnant Women
    Flow Cytometry
    Mothers
    Inflammation

    Cite this

    @article{4a0d88c75efe41ac9cdf9f2dd305b051,
    title = "T cell cytokine responses to stimulation with Ureaplasma parvum in pregnancy",
    abstract = "{\circledC} 2016 Elsevier Ireland Ltd.Ureaplasma spp. are a common vaginal microorganism causally linked to inflammation-driven preterm birth (PTB). The nature of the immune response to Ureaplasma spp. may influence PTB risk. This study sought to define maternal T cell cytokine responses to in vitro stimulation with Ureaplasma parvum serovar 3 (UpSV3) in vaginally colonised (UP+) and non-colonised (UP-) pregnant women. Whole blood flow cytometry demonstrated an increase (p = 0.027) in the baseline frequency of IFN?-positive CD3+CD4-(CD8+) T cells in UP+ women. UpSV3 stimulation resulted in a significant and specific increase (p = 0.001) in the frequency of IFN?-positive CD3+CD4-(CD8+) T cells, regardless of vaginal colonisation status. UpSV3 stimulation also increased the frequency of IFN?-positive CD3+CD4+ T cells, particularly in the UP+ group (p = 0.003). This is the first published study to examine T cell responses to Ureaplasma spp. exposure. Future appropriately-powered studies are needed to assess whether insufficient priming or a loss of tolerance to Ureaplasma spp. is occurring in UP+ women at risk of PTB.",
    author = "Peter Friedland and Lee-Pullen, {Tracey F.} and Nathan, {Liz A.} and Rory Watts and Keelan, {Jeffrey A.} and Payne, {Matt S.} and Ireland, {Demelza J.}",
    year = "2016",
    doi = "10.1016/j.jri.2016.03.002",
    language = "English",
    volume = "116",
    pages = "93--97",
    journal = "Journal of Reproductive Immunology",
    issn = "0165-0378",
    publisher = "Elsevier",

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    TY - JOUR

    T1 - T cell cytokine responses to stimulation with Ureaplasma parvum in pregnancy

    AU - Friedland, Peter

    AU - Lee-Pullen, Tracey F.

    AU - Nathan, Liz A.

    AU - Watts, Rory

    AU - Keelan, Jeffrey A.

    AU - Payne, Matt S.

    AU - Ireland, Demelza J.

    PY - 2016

    Y1 - 2016

    N2 - © 2016 Elsevier Ireland Ltd.Ureaplasma spp. are a common vaginal microorganism causally linked to inflammation-driven preterm birth (PTB). The nature of the immune response to Ureaplasma spp. may influence PTB risk. This study sought to define maternal T cell cytokine responses to in vitro stimulation with Ureaplasma parvum serovar 3 (UpSV3) in vaginally colonised (UP+) and non-colonised (UP-) pregnant women. Whole blood flow cytometry demonstrated an increase (p = 0.027) in the baseline frequency of IFN?-positive CD3+CD4-(CD8+) T cells in UP+ women. UpSV3 stimulation resulted in a significant and specific increase (p = 0.001) in the frequency of IFN?-positive CD3+CD4-(CD8+) T cells, regardless of vaginal colonisation status. UpSV3 stimulation also increased the frequency of IFN?-positive CD3+CD4+ T cells, particularly in the UP+ group (p = 0.003). This is the first published study to examine T cell responses to Ureaplasma spp. exposure. Future appropriately-powered studies are needed to assess whether insufficient priming or a loss of tolerance to Ureaplasma spp. is occurring in UP+ women at risk of PTB.

    AB - © 2016 Elsevier Ireland Ltd.Ureaplasma spp. are a common vaginal microorganism causally linked to inflammation-driven preterm birth (PTB). The nature of the immune response to Ureaplasma spp. may influence PTB risk. This study sought to define maternal T cell cytokine responses to in vitro stimulation with Ureaplasma parvum serovar 3 (UpSV3) in vaginally colonised (UP+) and non-colonised (UP-) pregnant women. Whole blood flow cytometry demonstrated an increase (p = 0.027) in the baseline frequency of IFN?-positive CD3+CD4-(CD8+) T cells in UP+ women. UpSV3 stimulation resulted in a significant and specific increase (p = 0.001) in the frequency of IFN?-positive CD3+CD4-(CD8+) T cells, regardless of vaginal colonisation status. UpSV3 stimulation also increased the frequency of IFN?-positive CD3+CD4+ T cells, particularly in the UP+ group (p = 0.003). This is the first published study to examine T cell responses to Ureaplasma spp. exposure. Future appropriately-powered studies are needed to assess whether insufficient priming or a loss of tolerance to Ureaplasma spp. is occurring in UP+ women at risk of PTB.

    U2 - 10.1016/j.jri.2016.03.002

    DO - 10.1016/j.jri.2016.03.002

    M3 - Article

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    JF - Journal of Reproductive Immunology

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    ER -