Projects per year
Abstract
In native systems, gene expression is regulated by RNA binding proteins. Such proteins have been the target of a great deal of recent research interest, due to the potential for harnessing these regulatory effects for the construction of new biotechnological tools. In particular, focus has been targeted on building synthetic RNA binding proteins for sequence-specific targeting of new RNA transcripts. Pentatricopeptide repeat (PPR) proteins make compelling candidates as synthetic RNA binding proteins to target and bind RNA transcripts of interest, due to their defined RNA binding “code”, modular structure, and native capability to deliver catalytic C-terminal domains. In this review, we present a summary of up-to-date understanding of RNA site recognition by PPR proteins, progress towards the design of synthetic PPR proteins for RNA targeting in vitro and in vivo, highlight key areas for further research around these proteins and present an outlook for future applications for synthetic PPR proteins as biotechnological tools.
Original language | English |
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Pages (from-to) | 19-26 |
Number of pages | 8 |
Journal | Methods |
Volume | 208 |
DOIs | |
Publication status | Published - Dec 2022 |
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Mastering pyrimidine editing in RNA
ARC Australian Research Council
1/01/20 → 31/12/24
Project: Research
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ARC Centre of Excellence in Plant Energy Biology 2014 (CPEB2)
Millar, H., Pogson, B., Tyerman, S., Small, I., Whelan, J., Borevitz, J., Lister, R., Atkin, O. & Munns, R.
ARC Australian Research Council
1/01/14 → 31/05/21
Project: Research