Switch in rod opsin gene expression in the European eel, Anguilla anguilla (L.)

A. J. Hope, J. C. Partridge, P. K. Hayes

Research output: Contribution to journalArticle

57 Citations (Scopus)

Abstract

The rod photoreceptors of the European eel, Anguilla anguilla (L.), alter their wavelength of maximum sensitivity (λ(max) from ca. 523 nm to ca. 482 nm at maturation, a switch involving the synthesis of a new visual pigment protein (opsin) that is inserted into the outer segments of existing rods. We artificially induced the switch in rod opsin production by the administration of hormones, and monitored the switch at the level of mRNA accumulation using radiolabelled oligonuleotides that hybridized differently to the two forms of eel rod opsin. The production of the deep-sea form of rod opsin was detected 6h after the first hormone injection, and the switch in rod opsin expression was complete within four weeks, at which time only the mRNA for the deep-sea opsin was detectable in the retinal cells. It is suggested that this system could be used as a tractable model for studying the regulatory control of opsin gene expression.

Original languageEnglish
Pages (from-to)869-874
Number of pages6
JournalProceedings of the Royal Society B: Biological Sciences
Volume265
Issue number1399
DOIs
Publication statusPublished - 22 May 1998
Externally publishedYes

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