In an intensive survey of lentil and chickpea fields and research stations plots carried out in the Punjab province of Pakistan in 1997, more than 6505 plant samples were collected and tested by tissue-blot immunoassay (TBIA) for the presence of 14 different viruses. Overall, about one in five plants of lentil was virus infected, 15 % of fields having incidences higher than 50 %. In contrast, incidences of infection were mostly low in chickpea. Pea seed-borne mosaic virus (PSbMV, genus Potyvirus, family Potyviridae) followed by Cucumber mosaic virus (CMV, genus Cucumovirus, family Bromoviridae), both of which are aphid-transmitted and seed-borne in chickpea and lentil, were the most abundant viruses in lentils, and also occurred in chickpea. Chickpea chlorotic dwarf virus (CCDV, genus Mastrevirus, family Geminiviridae), Beet western yellows virus (BWYV, genus Polerovirus, family Luteoviridae) and 'chickpea luteovirus' (family Luteoviridae) were common in chickpea fields and were present in lentil. Faba bean necrotic yellows virus (FBNYV, genus Nanovirus) was occasionally found in lentil and chickpea fields, and Alfalfa mosaic virus (AMV, genus Alfamovirus, family Bromoviridae) in chickpea fields. Counting plants with virus symptoms greatly underestimated virus incidence in lentil crops but was a better guide to virus occurrence in chickpea. Because of associated yield losses, the high incidences of infection in lentils are cause for concern for lentil production. Virus incidence was sometimes sufficient within individual chickpea fields to be of concern.
|Translated title of the contribution||Survey for viruses in lentil and chickpea crops in Pakistan|
|Number of pages||11|
|Journal||Zeitschrift fur Pflanzenkrankheiten und Pflanzenschutz|
|Publication status||Published - 1 Jan 2001|