Suppression subtraction hybridization and northern analysis reveal upregulation of heat shock, trkB, and sodium calcium exchanger genes following global cerebral ischemia in the rat

B.T. Majda, Bruno Meloni, N. Rixon, N.W. Knuckey

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Driver (sham-operated) and tester (ischemic) hippocampal cDNAs were subtracted, and the resulting ischemia-induced upregulated gene expression was verified by northern analysis. cDNAs isolated corresponded to (1) genes known to be upregulated following ischemia, (hsc70, hsp90, hsp105 and trkB) and (2) a gene not previously implicated with cerebral ischemia, sodium calcium exchanger (ncx). Furthermore, upregulation of these genes was demonstrated following preconditioning transient global ischemia. (C) 2001 Elsevier Science B V All rights reserved.
Original languageEnglish
Pages (from-to)173-179
JournalMolecular Brain Research
Volume93
DOIs
Publication statusPublished - 2001

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