Super high resolution for single molecule-sequence-based typing of classical HLA loci using ion torrent PGM

Takashi Shiina, Shingo Suzuki, Jerzy K. Kulski, Hidetoshi Inoko

Research output: Chapter in Book/Conference paperChapter

3 Citations (Scopus)

Abstract

Super high resolution-single molecule-sequence-based typing (SS-SBT) is an HLA DNA typing method to the field 4 level of allelic resolution (formerly known as 8-digit typing) to efficiently detect novel and null alleles without phase ambiguity by combination of long ranged PCR amplification and next-generation sequencing (NGS) technologies. In this chapter, we describe three basic steps, long ranged PCR, NGS, and allele assignment.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
EditorsSebastian Boegel
Place of PublicationUSA
PublisherHumana Press
Pages115-133
Number of pages19
ISBN (Electronic)9781493985463
ISBN (Print)9781493985456
DOIs
Publication statusPublished - 1 Jan 2018

Publication series

NameMethods in Molecular Biology
Volume1802
ISSN (Print)1064-3745

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