Super high resolution-single molecule-sequence-based typing (SS-SBT) is an HLA DNA typing method to the field 4 level of allelic resolution (formerly known as 8-digit typing) to efficiently detect novel and null alleles without phase ambiguity by combination of long ranged PCR amplification and next-generation sequencing (NGS) technologies. In this chapter, we describe three basic steps, long ranged PCR, NGS, and allele assignment.
|Title of host publication||Methods in Molecular Biology|
|Place of Publication||USA|
|Number of pages||19|
|Publication status||Published - 1 Jan 2018|
|Name||Methods in Molecular Biology|