Studying membrane transport processes by non-invasive microelectrodes: Basic principles and methods

Sergey Shabala, Lana Shabala, Ian Newman

Research output: Chapter in Book/Conference paperChapterpeer-review

3 Citations (Scopus)

Abstract

Non-invasive microelectrode flux measurement is a convenient tool to study membrane transport processes in plants in situ. Its high resolution (a few microns in space and several seconds in time) enables real-time studies of membrane transport processes at various levels of plant structural organization, from intact organs to various tissues, single cells or protoplasts derived from these cells. Being noninvasive, the technique allows flux measurements over several hours or days, even from rapidly moving tissues such as growing or nutating plant axial organs. Importantly, fluxes of several ions and neutral molecules can be measured concurrently, enabling not only quantitative estimates of rates of measured processes, but also providing some valuable information about the stoichiometry between activities of various membrane transporters and their time dependence. In this review, we revise the principles of non-invasive ion and neutral molecule flux measurements using the MIFE™ technique and discuss some methodological aspects of manufacturing, calibrating, and using flux measuring microelectrodes.

Original languageEnglish
Title of host publicationPlant Electrophysiology
Subtitle of host publicationMethods and Cell Electrophysiology
PublisherSpringer
Pages167-186
Number of pages20
ISBN (Electronic)9783642291197
ISBN (Print)364229118X, 9783642291180
DOIs
Publication statusPublished - 1 Feb 2012
Externally publishedYes

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