Study of the spatial point spread function with depth in two-photon microscopy

Andrew K. Dunn, Vincent Wallace, Maria Coleno, Peter So, Bruce J. Tromberg

Research output: Chapter in Book/Conference paperConference paperpeer-review

Abstract

The spatial point spread function is studied for two-photon fluorescence imaging in turbid media. Using a Monte Carlo based model the effects of tissue optical properties on the point spread function, fluorescence generation, and fluorescence collection are studied. The simulated results are compared to measured point spread functions at different depths within a scattering sample. Results indicate that the limiting factor in two-photon imaging is a loss of signal caused by scattering rather than a loss of resolution.

Original languageEnglish
Title of host publicationProceedings of SPIE - The International Society for Optical Engineering
PublisherSPIE
Pages112-119
Number of pages8
Volume3605
ISBN (Print)0819430757
Publication statusPublished - 1999
Externally publishedYes
EventProceedings of the 1999 Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing VI - San Jose, CA, USA
Duration: 24 Jan 199925 Jan 1999

Conference

ConferenceProceedings of the 1999 Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing VI
CitySan Jose, CA, USA
Period24/01/9925/01/99

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