Abstract
The spatial point spread function is studied for two-photon fluorescence imaging in turbid media. Using a Monte Carlo based model the effects of tissue optical properties on the point spread function, fluorescence generation, and fluorescence collection are studied. The simulated results are compared to measured point spread functions at different depths within a scattering sample. Results indicate that the limiting factor in two-photon imaging is a loss of signal caused by scattering rather than a loss of resolution.
Original language | English |
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Title of host publication | Proceedings of SPIE - The International Society for Optical Engineering |
Publisher | SPIE |
Pages | 112-119 |
Number of pages | 8 |
Volume | 3605 |
ISBN (Print) | 0819430757 |
Publication status | Published - 1999 |
Externally published | Yes |
Event | Proceedings of the 1999 Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing VI - San Jose, CA, USA Duration: 24 Jan 1999 → 25 Jan 1999 |
Conference
Conference | Proceedings of the 1999 Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing VI |
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City | San Jose, CA, USA |
Period | 24/01/99 → 25/01/99 |