Structured illumination microscopy of living cells

Liisa M. Hirvonen, Trevor A. Smith

Research output: Chapter in Book/Conference paperConference paper

Abstract

One of the recently proposed super-resolution microscopy techniques is structured illumination microscopy (SIM), where a fine grating is projected onto the sample, and the final image is reconstructed from a set of images taken at different grating positions. With this technique, resolution improvement by a factor of two can be achieved compared to conventional wide-field imaging without the need for pulsed lasers, scanning or special properties of the fluorophores. The latest results in the application of this technique to image different subcellular structures at high resolution will be presented.

Original languageEnglish
Title of host publicationConference on Lasers and Electro-Optics/Pacific Rim, CLEOPR 2011
Pages766-768
Number of pages3
Publication statusPublished - 1 Dec 2011
Externally publishedYes
EventConference on Lasers and Electro-Optics/Pacific Rim, CLEOPR 2011 - Sydney, Australia
Duration: 28 Aug 20111 Sep 2011

Publication series

NameOptics InfoBase Conference Papers
ISSN (Electronic)2162-2701

Conference

ConferenceConference on Lasers and Electro-Optics/Pacific Rim, CLEOPR 2011
CountryAustralia
CitySydney
Period28/08/111/09/11

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