Structured illumination microscopy of a living cell

Liisa M. Hirvonen, Kai Wicker, Ondrej Mandula, Rainer Heintzmann

Research output: Contribution to journalArticle

117 Citations (Scopus)

Abstract

Due to diffraction, the resolution of imaging emitted light in a fluorescence microscope is limited to about 200 nm in the lateral direction. Resolution improvement by a factor of two can be achieved using structured illumination, where a fine grating is projected onto the sample, and the final image is reconstructed from a set of images taken at different grating positions. Here we demonstrate that with the help of a spatial light modulator, this technique can be used for imaging slowly moving structures in living cells.

Original languageEnglish
Pages (from-to)807-812
Number of pages6
JournalEuropean Biophysics Journal
Volume38
Issue number6
DOIs
Publication statusPublished - 1 Jul 2009
Externally publishedYes

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