TY - JOUR
T1 - Structure of a mitochondrial supercomplex formed by respiratory-chain complexes I and III
AU - Dudkina, N.V.
AU - Eubel, Holger
AU - Keegstra, W.
AU - Boekema, E.J.
AU - Braun, H-P.
PY - 2005
Y1 - 2005
N2 - Mitochondria are central to the efficient provision of energy for eukaryotic cells. The oxidative-phosphorylation system of mitochondria consists of a series of five major membrane complexes: NADH–ubiquinone oxidoreductase (commonly known as complex I), succinate–ubiquinone oxidoreductase (complex II), ubiquinol–cytochrome c oxidoreductase (cytochrome bc1 complex or complex III), cytochrome c–O2 oxidoreductase (complex IV), and F1F0–ATP synthase (complex V). Several lines of evidence have recently suggested that complexes I and III–V might interact to form supercomplexes. However, because of their fragility, the structures of these supercomplexes are still unknown. A stable supercomplex consisting of complex I and dimeric complex III was purified from plant mitochondria. Structural characterization by single-particle EM indicates a specific type of interaction between monomeric complex I and dimeric complex III in a 1:1 ratio. We present a model for how complexes I and III are spatially organized within the I+III2 supercomplex.
AB - Mitochondria are central to the efficient provision of energy for eukaryotic cells. The oxidative-phosphorylation system of mitochondria consists of a series of five major membrane complexes: NADH–ubiquinone oxidoreductase (commonly known as complex I), succinate–ubiquinone oxidoreductase (complex II), ubiquinol–cytochrome c oxidoreductase (cytochrome bc1 complex or complex III), cytochrome c–O2 oxidoreductase (complex IV), and F1F0–ATP synthase (complex V). Several lines of evidence have recently suggested that complexes I and III–V might interact to form supercomplexes. However, because of their fragility, the structures of these supercomplexes are still unknown. A stable supercomplex consisting of complex I and dimeric complex III was purified from plant mitochondria. Structural characterization by single-particle EM indicates a specific type of interaction between monomeric complex I and dimeric complex III in a 1:1 ratio. We present a model for how complexes I and III are spatially organized within the I+III2 supercomplex.
U2 - 10.1073/pnas.0408870102
DO - 10.1073/pnas.0408870102
M3 - Article
SN - 0027-8424
VL - 102
SP - 3225
EP - 3229
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
IS - 9
ER -