Structure illumination microscopy imaging of lipid vesicles in live bacteria with naphthalimide-appended organometallic complexes

Anna Maria Ranieri, Matteo Vezzelli, Kathryn G. Leslie, Song Huang, Stefano Stagni, Denis Jacquemin, Haibo Jiang, Alysia Hubbard, Luca Rigamonti, Elizabeth L.J. Watkin, Mark I. Ogden, Elizabeth J. New, Massimiliano Massi

Research output: Contribution to journalArticlepeer-review

Abstract

There is a lack of molecular probes for imaging bacteria, in comparison to the array of such tools available for the imaging of mammalian cells. Here, organometallic molecular probes have been developed and assessed for bacterial imaging, designed to have the potential to support multiple imaging modalities. The chemical structure of the probes is designed around a metal-naphthalimide structure. The 4-amino-1,8-naphthalimide moiety, covalently appended through a pyridine ancillary ligand, acts as a luminescent probe for super-resolution microscopy. On the other hand, the metal centre, rhenium(i) or platinum(ii) in the current study, enables techniques such as nanoSIMS. While the rhenium(i) complex was not sufficiently stable to be used as a probe, the platinum(ii) analogue showed good chemical and biological stability. Structured illumination microscopy (SIM) imaging on live Bacillus cereus confirmed the suitability of the probe for super-resolution microscopy. NanoSIMS analysis was used to monitor the uptake of the platinum(ii) complex within the bacteria and demonstrate the potential of this chemical architecture to enable multimodal imaging. The successful combination of these two moieties introduces a platform that could lead to a versatile range of multi-functional probes for bacteria. This journal is

Original languageEnglish
Pages (from-to)3818-3822
Number of pages5
JournalAnalyst
Volume146
Issue number12
DOIs
Publication statusPublished - 21 Jun 2021

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