Projects per year
Abstract
An important trait associated with the salt tolerance of wheat is the exclusion of sodium ions (Na+) from the shoot. We have previously shown that the sodium transporters TmHKT1;5-A and TaHKT1;5-D, from Triticum monoccocum (Tm) and Triticum aestivum (Ta), are encoded by genes underlying the major shoot Na+-exclusion loci Nax1 and Kna1, respectively. Here, using heterologous expression, we show that the affinity (Km) for the Na+ transport of TmHKT1;5-A, at 2.66 mM, is higher than that of TaHKT1;5-D at 7.50 mM. Through 3D structural modelling, we identify residues D471/a gap and D474/G473 that contribute to this property. We identify four additional mutations in amino acid residues that inhibit the transport activity of TmHKT1;5-A, which are predicted to be the result of an occlusion of the pore. We propose that the underlying transport properties of TmHKT1;5-A and TaHKT1;5-D contribute to their unique ability to improve Na+ exclusion in wheat that leads to an improved salinity tolerance in the field.
Original language | English |
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Pages (from-to) | 1133-1144 |
Number of pages | 12 |
Journal | Cellular and Molecular Life Sciences |
Volume | 75 |
Issue number | 6 |
Early online date | 27 Nov 2017 |
DOIs | |
Publication status | Published - Mar 2018 |
Fingerprint Dive into the research topics of 'Structural variations in wheat HKT1;5 underpin differences in Na<sup>+</sup> transport capacity'. Together they form a unique fingerprint.
Projects
- 1 Active
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ARC Centre of Excellence in Plant Energy Biology 2014 (CPEB2)
Millar, H., Pogson, B., Tyerman, S., Small, I., Whelan, J., Borevitz, J., Lister, R., Atkin, O. & Munns, R.
1/01/14 → 31/05/21
Project: Research