Structural variations in wheat HKT1;5 underpin differences in Na+ transport capacity

Bo Xu, Shane Waters, Caitlin S. Byrt, Darren Plett, Stephen D. Tyerman, Mark Tester, Rana Munns, Maria Hrmova, Matthew Gilliham

Research output: Contribution to journalArticle

17 Citations (Scopus)
277 Downloads (Pure)

Abstract

An important trait associated with the salt tolerance of wheat is the exclusion of sodium ions (Na+) from the shoot. We have previously shown that the sodium transporters TmHKT1;5-A and TaHKT1;5-D, from Triticum monoccocum (Tm) and Triticum aestivum (Ta), are encoded by genes underlying the major shoot Na+-exclusion loci Nax1 and Kna1, respectively. Here, using heterologous expression, we show that the affinity (Km) for the Na+ transport of TmHKT1;5-A, at 2.66 mM, is higher than that of TaHKT1;5-D at 7.50 mM. Through 3D structural modelling, we identify residues D471/a gap and D474/G473 that contribute to this property. We identify four additional mutations in amino acid residues that inhibit the transport activity of TmHKT1;5-A, which are predicted to be the result of an occlusion of the pore. We propose that the underlying transport properties of TmHKT1;5-A and TaHKT1;5-D contribute to their unique ability to improve Na+ exclusion in wheat that leads to an improved salinity tolerance in the field.

Original languageEnglish
Pages (from-to)1133-1144
Number of pages12
JournalCellular and Molecular Life Sciences
Volume75
Issue number6
Early online date27 Nov 2017
DOIs
Publication statusPublished - Mar 2018

Fingerprint Dive into the research topics of 'Structural variations in wheat HKT1;5 underpin differences in Na<sup>+</sup> transport capacity'. Together they form a unique fingerprint.

Cite this