Structural characterization of polysaccharide-coated iron oxide nanoparticles produced by Staphylococcus warneri, isolated from a thermal spring

Sedigheh Kianpour, Alireza Ebrahiminezhad, Maryam Deyhimi, Manica Negahdaripour, Mohammad Javad Raee, Milad Mohkam, Hamideh Rezaee, Cambyz Irajie, Aydin Berenjian, Younes Ghasemi

Research output: Contribution to journalArticle

Abstract

The biocompatible-coated iron oxide nanoparticles (IONs) have attracted a great interest because of their various applications in biological science and medicine. In most cases, the toxic effect of naked iron oxide nanoparticles is completely cleared by adding a biocompatible coating, such as polysaccharides, polyethylene glycol (PEG), or biosynthesis of biocompatible-coated IONs using microorganisms such as bacteria. In the present study, polysaccharide-coated iron oxide nanoparticles were produced by a strain of Staphylococcus warneri isolated from a thermal spring. For identification of the isolated bacterium, 16S rRNA gene sequencing was done. Characterization of the nanoparticles was performed for the first time, using transmission electron microscopy (TEM), dynamic light scattering (DLS), thermogravimetric analysis (TGA), X-ray crystallography (XRD), Fourier-transform infrared (FTIR) spectroscopy, vibrating sample magnetometer (VSM), and 3-(4,5-dimethylthiazol-2-yl)−2,5-diphenyltetrazolium bromide (MTT) assay. Results indicated that the spherical iron oxide nanoparticles were coated by a polysaccharide (13.6%), which provided a large negative charge of −91 mV and very low saturation magnetization of around 0.28 emu/g. The result of MTT assay on MOLT-4 cell lines showed that the percentage of viability was between 95.6% and 68.9% in the 10–100 µM of nanoparticle concentrations with a high IC 50 value, which makes it appropriate for biomedical applications such as cancer therapy.

Original languageEnglish
Pages (from-to)569-578
Number of pages10
JournalJournal of Basic Microbiology
Volume59
Issue number6
DOIs
Publication statusPublished - 1 Jun 2019

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Staphylococcus
Nanoparticles
Polysaccharides
Hot Temperature
Dromaiidae
Bacteria
Biological Science Disciplines
Poisons
X Ray Crystallography
Fourier Transform Infrared Spectroscopy
ferric oxide
Transmission Electron Microscopy
rRNA Genes
Medicine
Cell Line
Neoplasms

Cite this

Kianpour, Sedigheh ; Ebrahiminezhad, Alireza ; Deyhimi, Maryam ; Negahdaripour, Manica ; Raee, Mohammad Javad ; Mohkam, Milad ; Rezaee, Hamideh ; Irajie, Cambyz ; Berenjian, Aydin ; Ghasemi, Younes. / Structural characterization of polysaccharide-coated iron oxide nanoparticles produced by Staphylococcus warneri, isolated from a thermal spring. In: Journal of Basic Microbiology. 2019 ; Vol. 59, No. 6. pp. 569-578.
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abstract = "The biocompatible-coated iron oxide nanoparticles (IONs) have attracted a great interest because of their various applications in biological science and medicine. In most cases, the toxic effect of naked iron oxide nanoparticles is completely cleared by adding a biocompatible coating, such as polysaccharides, polyethylene glycol (PEG), or biosynthesis of biocompatible-coated IONs using microorganisms such as bacteria. In the present study, polysaccharide-coated iron oxide nanoparticles were produced by a strain of Staphylococcus warneri isolated from a thermal spring. For identification of the isolated bacterium, 16S rRNA gene sequencing was done. Characterization of the nanoparticles was performed for the first time, using transmission electron microscopy (TEM), dynamic light scattering (DLS), thermogravimetric analysis (TGA), X-ray crystallography (XRD), Fourier-transform infrared (FTIR) spectroscopy, vibrating sample magnetometer (VSM), and 3-(4,5-dimethylthiazol-2-yl)−2,5-diphenyltetrazolium bromide (MTT) assay. Results indicated that the spherical iron oxide nanoparticles were coated by a polysaccharide (13.6{\%}), which provided a large negative charge of −91 mV and very low saturation magnetization of around 0.28 emu/g. The result of MTT assay on MOLT-4 cell lines showed that the percentage of viability was between 95.6{\%} and 68.9{\%} in the 10–100 µM of nanoparticle concentrations with a high IC 50 value, which makes it appropriate for biomedical applications such as cancer therapy.",
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Kianpour, S, Ebrahiminezhad, A, Deyhimi, M, Negahdaripour, M, Raee, MJ, Mohkam, M, Rezaee, H, Irajie, C, Berenjian, A & Ghasemi, Y 2019, 'Structural characterization of polysaccharide-coated iron oxide nanoparticles produced by Staphylococcus warneri, isolated from a thermal spring' Journal of Basic Microbiology, vol. 59, no. 6, pp. 569-578. https://doi.org/10.1002/jobm.201800684

Structural characterization of polysaccharide-coated iron oxide nanoparticles produced by Staphylococcus warneri, isolated from a thermal spring. / Kianpour, Sedigheh; Ebrahiminezhad, Alireza; Deyhimi, Maryam; Negahdaripour, Manica; Raee, Mohammad Javad; Mohkam, Milad; Rezaee, Hamideh; Irajie, Cambyz; Berenjian, Aydin; Ghasemi, Younes.

In: Journal of Basic Microbiology, Vol. 59, No. 6, 01.06.2019, p. 569-578.

Research output: Contribution to journalArticle

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T1 - Structural characterization of polysaccharide-coated iron oxide nanoparticles produced by Staphylococcus warneri, isolated from a thermal spring

AU - Kianpour, Sedigheh

AU - Ebrahiminezhad, Alireza

AU - Deyhimi, Maryam

AU - Negahdaripour, Manica

AU - Raee, Mohammad Javad

AU - Mohkam, Milad

AU - Rezaee, Hamideh

AU - Irajie, Cambyz

AU - Berenjian, Aydin

AU - Ghasemi, Younes

PY - 2019/6/1

Y1 - 2019/6/1

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