TY - BOOK
T1 - Structural characterisation of capsular transporter proteins from Neisseria meningitidis
AU - Masselot--Joubert, Lorelei
PY - 2023
Y1 - 2023
N2 - Neisseria meningitidis (Nm) uses a polysaccharide capsule to protect itself and escape the immune system. Polysaccharide transporters are complexes inserted into bacterial membranes exporting polysaccharides, of which the Nm ABC-transporter-dependent system (CtrA, CtrB, CtrC and CtrD) is one. This thesis presents: the crystallographic structure of CtrC (transmembrane domain of the ABC transporter), the low-resolution TEM structure of CtrB (polysaccharide co-polymerase protein) in detergent, the interactions between CtrB and CtrC and the CtrB/CtrC low-resolution TEM structure in circularised nanodiscs.
AB - Neisseria meningitidis (Nm) uses a polysaccharide capsule to protect itself and escape the immune system. Polysaccharide transporters are complexes inserted into bacterial membranes exporting polysaccharides, of which the Nm ABC-transporter-dependent system (CtrA, CtrB, CtrC and CtrD) is one. This thesis presents: the crystallographic structure of CtrC (transmembrane domain of the ABC transporter), the low-resolution TEM structure of CtrB (polysaccharide co-polymerase protein) in detergent, the interactions between CtrB and CtrC and the CtrB/CtrC low-resolution TEM structure in circularised nanodiscs.
KW - Neisseria meningitidis
KW - ABC-transporter-dependent system
KW - Polysaccharide transporter
KW - Polysaccharide capsule
KW - X-ray crystallography
KW - Transmission electron microscopy
KW - Bacterial membrane protein
KW - Structural biology
U2 - 10.26182/93gm-xp13
DO - 10.26182/93gm-xp13
M3 - Doctoral Thesis
ER -