Structural and Functional Characterisation of Designer PPR RNA Editing Factors

Santana Royan

doi:10.26182/5f31fc788d8fa

Structural and Functional Characterisation of Designer PPR RNA Editing Factors

Santana Royan

School of Molecular Sciences

Research output: Thesis › Doctoral Thesis

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Abstract

Members of the pentatricopeptide repeat (PPR) protein family catalyse targeted RNA editing in land plants. This thesis describes the structural and functional characterisation of novel designer PPR RNA editing factors based on these natural proteins. Utilising a combination of structural modelling and small angle X-ray scattering data, structural models of these factors and their complexes were produced and refined. Site-specific RNA editing catalysed by these factors was demonstrated in the plant Arabidopsis thaliana and in the bacterium E. coli. This study serves as a successful pilot into the design and application of programmable PPR-based RNA editing factors.

Original language	English
Qualification	Doctor of Philosophy
Awarding Institution	The University of Western Australia
Supervisors/Advisors	Gutmann, Bernard, Supervisor Schmidberger, Jason, Supervisor Bond, Charlie, Supervisor Small, Ian, Supervisor
Thesis sponsors	Australian Government Research Training Program
Award date	12 Jun 2020
DOIs	https://doi.org/10.26182/5f31fc788d8fa
Publication status	Unpublished - 2019

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10.26182/5f31fc788d8fa

THESIS - DOCTOR OF PHILOSOPHY - ROYAN, Santana Ashanth - 2019
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Cite this

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title = "Structural and Functional Characterisation of Designer PPR RNA Editing Factors",

abstract = "Members of the pentatricopeptide repeat (PPR) protein family catalyse targeted RNA editing in land plants. This thesis describes the structural and functional characterisation of novel designer PPR RNA editing factors based on these natural proteins. Utilising a combination of structural modelling and small angle X-ray scattering data, structural models of these factors and their complexes were produced and refined. Site-specific RNA editing catalysed by these factors was demonstrated in the plant Arabidopsis thaliana and in the bacterium E. coli. This study serves as a successful pilot into the design and application of programmable PPR-based RNA editing factors.",

keywords = "RNA editing, PPR proteins, Designer proteins, Structural biology, Synthetic biology, SAXS",

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language = "English",

school = "The University of Western Australia",

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N2 - Members of the pentatricopeptide repeat (PPR) protein family catalyse targeted RNA editing in land plants. This thesis describes the structural and functional characterisation of novel designer PPR RNA editing factors based on these natural proteins. Utilising a combination of structural modelling and small angle X-ray scattering data, structural models of these factors and their complexes were produced and refined. Site-specific RNA editing catalysed by these factors was demonstrated in the plant Arabidopsis thaliana and in the bacterium E. coli. This study serves as a successful pilot into the design and application of programmable PPR-based RNA editing factors.

AB - Members of the pentatricopeptide repeat (PPR) protein family catalyse targeted RNA editing in land plants. This thesis describes the structural and functional characterisation of novel designer PPR RNA editing factors based on these natural proteins. Utilising a combination of structural modelling and small angle X-ray scattering data, structural models of these factors and their complexes were produced and refined. Site-specific RNA editing catalysed by these factors was demonstrated in the plant Arabidopsis thaliana and in the bacterium E. coli. This study serves as a successful pilot into the design and application of programmable PPR-based RNA editing factors.

KW - RNA editing

KW - PPR proteins

KW - Designer proteins

KW - Structural biology

KW - Synthetic biology

KW - SAXS

U2 - 10.26182/5f31fc788d8fa

DO - 10.26182/5f31fc788d8fa

M3 - Doctoral Thesis

ER -

Structural and Functional Characterisation of Designer PPR RNA Editing Factors

Abstract

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