Structural and Functional Characterisation of Designer PPR RNA Editing Factors

Santana Royan

Research output: ThesisDoctoral Thesis

178 Downloads (Pure)

Abstract

Members of the pentatricopeptide repeat (PPR) protein family catalyse targeted RNA editing in land plants. This thesis describes the structural and functional characterisation of novel designer PPR RNA editing factors based on these natural proteins. Utilising a combination of structural modelling and small angle X-ray scattering data, structural models of these factors and their complexes were produced and refined. Site-specific RNA editing catalysed by these factors was demonstrated in the plant Arabidopsis thaliana and in the bacterium E. coli. This study serves as a successful pilot into the design and application of programmable PPR-based RNA editing factors.
Original languageEnglish
QualificationDoctor of Philosophy
Awarding Institution
  • The University of Western Australia
Supervisors/Advisors
  • Gutmann, Bernard, Supervisor
  • Schmidberger, Jason, Supervisor
  • Bond, Charlie, Supervisor
  • Small, Ian, Supervisor
Thesis sponsors
Award date12 Jun 2020
DOIs
Publication statusUnpublished - 2019

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