TY - JOUR
T1 - Stimulation of human synovial fibroblast DNA synthesis by recombinant human cytokines
AU - Butler, D. M.
AU - Piccoli, D. S.
AU - Hart, P. H.
AU - Hamilton, J. A.
PY - 1988/1/1
Y1 - 1988/1/1
N2 - The pronounced synovial hyperplasia often found in rheumatoid joints can be explained at least partially, by the interaction of monocyte-macrophage polypeptides (monokines) and lymphocyte polypeptides (lymphokines) with synovial fibroblast-like cells. We now report that purified recombinant human cytokines, interleukin-1α, interleukin-1β, tumor necrosis factor α, tumor necrosis factor β (or lymphotoxin) and, to a variable extent, interferon-γ, stimulate the DNA synthesis of human synovial fibroblast-like cells cultured in low (i.e., 1%) fetal bovine serum. With the exception of interferon-γ, the effects of the cytokines were generally elevated by indomethacin, suggesting inhibition of the DNA synthesis by an endogenously produced cyclooxygenase product(s). Consistent with this suggestion, exogenous prostaglandin E2 suppressed cytokine induced DNA synthesis. All of the cytokines studied might play a role in the synovial hyperplasia present in rheumatoid disease.
AB - The pronounced synovial hyperplasia often found in rheumatoid joints can be explained at least partially, by the interaction of monocyte-macrophage polypeptides (monokines) and lymphocyte polypeptides (lymphokines) with synovial fibroblast-like cells. We now report that purified recombinant human cytokines, interleukin-1α, interleukin-1β, tumor necrosis factor α, tumor necrosis factor β (or lymphotoxin) and, to a variable extent, interferon-γ, stimulate the DNA synthesis of human synovial fibroblast-like cells cultured in low (i.e., 1%) fetal bovine serum. With the exception of interferon-γ, the effects of the cytokines were generally elevated by indomethacin, suggesting inhibition of the DNA synthesis by an endogenously produced cyclooxygenase product(s). Consistent with this suggestion, exogenous prostaglandin E2 suppressed cytokine induced DNA synthesis. All of the cytokines studied might play a role in the synovial hyperplasia present in rheumatoid disease.
UR - http://www.scopus.com/inward/record.url?scp=0023767680&partnerID=8YFLogxK
M3 - Article
C2 - 3264575
AN - SCOPUS:0023767680
VL - 15
SP - 1463
EP - 1470
JO - Journal of Rheumatology
JF - Journal of Rheumatology
SN - 0315-162X
IS - 10
ER -