Stimulation of HIV-specific T cell clonotypes using allogeneic HLA

Coral Ann Almeida, Paula van Miert, Kane O'Driscoll, Yvonne M. Zoet, Abha Chopra, Mark Watson, Dianne de Santis, Campbell Witt, Mina John, Frans H.J. Claas, Lloyd J. D'Orsogna

    Research output: Contribution to journalArticle

    5 Citations (Scopus)

    Abstract

    We hypothesized that HIV-specific CD8 T cell clonotypes can be stimulated by allogeneic HLA molecules. Multiple HIV-specific CD8 T cell clones were derived from 12 individuals with chronic HIV infection, specific for 13 different HIV Gag antigens and restricted to 7 different HLA molecules. The generated T cell clones were assayed for alloreactivity against a panel of single HLA class I expressing cell lines (SALs). HIV-specific T cells recognising at least one allogeneic HLA molecule could be identified from 7 of 12 patients tested. Allorecognition was associated with IFNγ cytokine production, CD137 upregulation and cytotoxicity, suggesting high avidity allo-stimulation. Allo-HLA recognition by HIV-specific T cells was specific to the HIV target peptide/HLA restriction and TCR TRBV usage of the T cells. HIV-specific T cells do crossreact against allogeneic HLA molecules in an epitope and TRBV specific manner. Therefore allo-HLA stimulation could be exploited to induce or augment HIV-specific T cell responses.

    Original languageEnglish
    Pages (from-to)32-40
    JournalCellular Immunology
    Volume316
    DOIs
    Publication statusPublished - Jun 2017

    Fingerprint

    HIV
    T-Lymphocytes
    Clone Cells
    HIV Antigens
    gag Gene Products
    HIV Infections
    Epitopes
    Up-Regulation
    Cytokines
    Cell Line
    Peptides

    Cite this

    Almeida, C. A., van Miert, P., O'Driscoll, K., Zoet, Y. M., Chopra, A., Watson, M., ... D'Orsogna, L. J. (2017). Stimulation of HIV-specific T cell clonotypes using allogeneic HLA. Cellular Immunology, 316, 32-40. https://doi.org/10.1016/j.cellimm.2017.03.004
    Almeida, Coral Ann ; van Miert, Paula ; O'Driscoll, Kane ; Zoet, Yvonne M. ; Chopra, Abha ; Watson, Mark ; de Santis, Dianne ; Witt, Campbell ; John, Mina ; Claas, Frans H.J. ; D'Orsogna, Lloyd J. / Stimulation of HIV-specific T cell clonotypes using allogeneic HLA. In: Cellular Immunology. 2017 ; Vol. 316. pp. 32-40.
    @article{6907ab88171b4788a695be8253ac3fda,
    title = "Stimulation of HIV-specific T cell clonotypes using allogeneic HLA",
    abstract = "We hypothesized that HIV-specific CD8 T cell clonotypes can be stimulated by allogeneic HLA molecules. Multiple HIV-specific CD8 T cell clones were derived from 12 individuals with chronic HIV infection, specific for 13 different HIV Gag antigens and restricted to 7 different HLA molecules. The generated T cell clones were assayed for alloreactivity against a panel of single HLA class I expressing cell lines (SALs). HIV-specific T cells recognising at least one allogeneic HLA molecule could be identified from 7 of 12 patients tested. Allorecognition was associated with IFNγ cytokine production, CD137 upregulation and cytotoxicity, suggesting high avidity allo-stimulation. Allo-HLA recognition by HIV-specific T cells was specific to the HIV target peptide/HLA restriction and TCR TRBV usage of the T cells. HIV-specific T cells do crossreact against allogeneic HLA molecules in an epitope and TRBV specific manner. Therefore allo-HLA stimulation could be exploited to induce or augment HIV-specific T cell responses.",
    keywords = "Allostimulation, Crossreactivity, HIV, HLA, Memory T cells, Vaccine",
    author = "Almeida, {Coral Ann} and {van Miert}, Paula and Kane O'Driscoll and Zoet, {Yvonne M.} and Abha Chopra and Mark Watson and {de Santis}, Dianne and Campbell Witt and Mina John and Claas, {Frans H.J.} and D'Orsogna, {Lloyd J.}",
    year = "2017",
    month = "6",
    doi = "10.1016/j.cellimm.2017.03.004",
    language = "English",
    volume = "316",
    pages = "32--40",
    journal = "Cellular Immunology",
    issn = "0008-8749",
    publisher = "Academic Press",

    }

    Almeida, CA, van Miert, P, O'Driscoll, K, Zoet, YM, Chopra, A, Watson, M, de Santis, D, Witt, C, John, M, Claas, FHJ & D'Orsogna, LJ 2017, 'Stimulation of HIV-specific T cell clonotypes using allogeneic HLA' Cellular Immunology, vol. 316, pp. 32-40. https://doi.org/10.1016/j.cellimm.2017.03.004

    Stimulation of HIV-specific T cell clonotypes using allogeneic HLA. / Almeida, Coral Ann; van Miert, Paula; O'Driscoll, Kane; Zoet, Yvonne M.; Chopra, Abha; Watson, Mark; de Santis, Dianne; Witt, Campbell; John, Mina; Claas, Frans H.J.; D'Orsogna, Lloyd J.

    In: Cellular Immunology, Vol. 316, 06.2017, p. 32-40.

    Research output: Contribution to journalArticle

    TY - JOUR

    T1 - Stimulation of HIV-specific T cell clonotypes using allogeneic HLA

    AU - Almeida, Coral Ann

    AU - van Miert, Paula

    AU - O'Driscoll, Kane

    AU - Zoet, Yvonne M.

    AU - Chopra, Abha

    AU - Watson, Mark

    AU - de Santis, Dianne

    AU - Witt, Campbell

    AU - John, Mina

    AU - Claas, Frans H.J.

    AU - D'Orsogna, Lloyd J.

    PY - 2017/6

    Y1 - 2017/6

    N2 - We hypothesized that HIV-specific CD8 T cell clonotypes can be stimulated by allogeneic HLA molecules. Multiple HIV-specific CD8 T cell clones were derived from 12 individuals with chronic HIV infection, specific for 13 different HIV Gag antigens and restricted to 7 different HLA molecules. The generated T cell clones were assayed for alloreactivity against a panel of single HLA class I expressing cell lines (SALs). HIV-specific T cells recognising at least one allogeneic HLA molecule could be identified from 7 of 12 patients tested. Allorecognition was associated with IFNγ cytokine production, CD137 upregulation and cytotoxicity, suggesting high avidity allo-stimulation. Allo-HLA recognition by HIV-specific T cells was specific to the HIV target peptide/HLA restriction and TCR TRBV usage of the T cells. HIV-specific T cells do crossreact against allogeneic HLA molecules in an epitope and TRBV specific manner. Therefore allo-HLA stimulation could be exploited to induce or augment HIV-specific T cell responses.

    AB - We hypothesized that HIV-specific CD8 T cell clonotypes can be stimulated by allogeneic HLA molecules. Multiple HIV-specific CD8 T cell clones were derived from 12 individuals with chronic HIV infection, specific for 13 different HIV Gag antigens and restricted to 7 different HLA molecules. The generated T cell clones were assayed for alloreactivity against a panel of single HLA class I expressing cell lines (SALs). HIV-specific T cells recognising at least one allogeneic HLA molecule could be identified from 7 of 12 patients tested. Allorecognition was associated with IFNγ cytokine production, CD137 upregulation and cytotoxicity, suggesting high avidity allo-stimulation. Allo-HLA recognition by HIV-specific T cells was specific to the HIV target peptide/HLA restriction and TCR TRBV usage of the T cells. HIV-specific T cells do crossreact against allogeneic HLA molecules in an epitope and TRBV specific manner. Therefore allo-HLA stimulation could be exploited to induce or augment HIV-specific T cell responses.

    KW - Allostimulation

    KW - Crossreactivity

    KW - HIV

    KW - HLA

    KW - Memory T cells

    KW - Vaccine

    UR - http://www.scopus.com/inward/record.url?scp=85016402578&partnerID=8YFLogxK

    U2 - 10.1016/j.cellimm.2017.03.004

    DO - 10.1016/j.cellimm.2017.03.004

    M3 - Article

    VL - 316

    SP - 32

    EP - 40

    JO - Cellular Immunology

    JF - Cellular Immunology

    SN - 0008-8749

    ER -

    Almeida CA, van Miert P, O'Driscoll K, Zoet YM, Chopra A, Watson M et al. Stimulation of HIV-specific T cell clonotypes using allogeneic HLA. Cellular Immunology. 2017 Jun;316:32-40. https://doi.org/10.1016/j.cellimm.2017.03.004