Staphylococcal Enterotoxin-B-Mediated Stimulation of Interleukin-13 Production as a Potential Aetiologic Factor in Eczema in Infants

H.S. Lehmann, T. Heaton, D. Mallon, Patrick Holt

Research output: Contribution to journalArticlepeer-review

29 Citations (Scopus)

Abstract

Background: Staphylococcus aureus colonization has been found in 80 - 100% of lesional skin from patients with atopic eczema dermatitis syndrome (AEDS) and is thought to have a role in the pathogenesis of the disease. Furthermore, up to 65% of S. aureus from lesional skin has been shown to produce toxigenic superantigens. Methods: Using a cohort of 11 children under 2 years of age diagnosed with AEDS, we isolated peripheral blood mononuclear cells, cultured them with staphylococcal enterotoxin B (SEB) and phytohaemagglutinin, and assessed the cytokine response profiles. Plasma was also collected for immunoglobulin E analysis. In addition, skin and nasal swabs were taken and cultured to determine the presence of SEB-producing S. aureus by polymerase chain reaction (PCR) and reverse passive latex agglutination. Results: We found a significant increase in the production of the SEB-induced cytokines interleukin (IL)-5 and IL-13 in the patient group when compared with non-atopic, healthy controls. For IL-13, there was almost no overlap in the levels between the groups. However, there was no correlation between SEB-induced IL-13 and disease severity. This difference was not seen when heat-inactivated S. aureus was used to stimulate the cells. Conclusions: IL-13 is an important factor in AEDS development in early childhood, and prophylactic antistaphylococcal treatment may provide protection from AEDS in atopic individuals. Copyright (C) 2004 S. Karger AG, Basel.
Original languageEnglish
Pages (from-to)306-312
JournalInternational Archives of Allergy and Immunology
Volume135
DOIs
Publication statusPublished - 2004

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