@article{5a591a98abc6463bbf7016c371ce8875,
title = "Stalk cell polar ion transport provide for bladder-based salinity tolerance in Chenopodium quinoa",
abstract = "Chenopodium quinoa uses epidermal bladder cells (EBCs) to sequester excess salt. Each EBC complex consists of a leaf epidermal cell, a stalk cell, and the bladder. Under salt stress, sodium (Na+), chloride (Cl−), potassium (K+) and various metabolites are shuttled from the leaf lamina to the bladders. Stalk cells operate as both a selectivity filter and a flux controller. In line with the nature of a transfer cell, advanced transmission electron tomography, electrophysiology, and fluorescent tracer flux studies revealed the stalk cell{\textquoteright}s polar organization and bladder-directed solute flow. RNA sequencing and cluster analysis revealed the gene expression profiles of the stalk cells. Among the stalk cell enriched genes, ion channels and carriers as well as sugar transporters were most pronounced. Based on their electrophysiological fingerprint and thermodynamic considerations, a model for stalk cell transcellular transport was derived.",
keywords = "halophyte, polar ion transport, quinoa, salt tolerance, stalk cell",
author = "Nadia Bazihizina and Jennifer B{\"o}hm and Maxim Messerer and Christian Stigloher and M{\"u}ller, \{Heike M.\} and Cuin, \{Tracey Ann\} and Tobias Maierhofer and Joan Cabot and Mayer, \{Klaus F.X.\} and Christian Fella and Shouguang Huang and Al-Rasheid, \{Khaled A.S.\} and Saleh Alquraishi and Michael Breadmore and Stefano Mancuso and Sergey Shabala and Peter Ache and Heng Zhang and Zhu, \{Jian Kang\} and Rainer Hedrich and S{\"o}nke Scherzer",
note = "Funding Information: We wish to thank Daniela Bunsen (University of Wuerzburg) for her assistance in preparing the samples for the 3D‐EM. This work was supported by the DFG (Deutsche Forschungs Gemeinschaft) grant SCHE 2148/1‐1 to S Scherzer and grant HE 1640/44‐1 to RH, the DFG – INST 93/1003‐1 FUGG (426173797), the Strategic Priority Research Program of CAS (XDB27040108 to HZ and XDB27040101 to J‐KZ), the King Saud University´s International Cooperation and Scientific Twinning Dept., Riyadh, Saudi Arabia (Project ICSTD‐2020/2), the King Saud University's Distinguished Fellowship Program, Riyadh, Saudi Arabia to KFXM, the Australian Research Council (DP150101663), National Distinguished Expert Project (WQ20174400441), and grant no. 31961143001 for Joint Research Projects between the the Pakistan Science Foundation and the National Natural Science Foundation China to S Shabala. NB was a recipient of the Marie Curie Fellowship funded by the EU (grant no. 700001). Publisher Copyright: {\textcopyright} 2022 The Authors. New Phytologist {\textcopyright} 2022 New Phytologist Foundation.",
year = "2022",
month = sep,
doi = "10.1111/nph.18205",
language = "English",
volume = "235",
pages = "1822--1835",
journal = "New Phytologist",
issn = "0028-646X",
publisher = "Wiley-Blackwell",
number = "5",
}