Stable conditional expression and effect of C/EBPβ-LIP in adipocytes using the pSLIK system

Cristina L. Esteves, Val Kelly, Valérie Bégay, Simon G. Lillico, Achim Leutz, Jonathan R. Seck, Karen E. Chapman

Research output: Contribution to journalArticle

4 Citations (Scopus)
94 Downloads (Pure)

Abstract

Murine 3T3-L1 adipocytes are widely used as a cellular model of obesity. However, whereas transfection of 3T3-L1 preadipocytes is straightforward, ectopic gene expression in mature 3T3-L1 adipocytes has proved challenging. Here, we used the pSLIK vector system to generate stable doxycycline-inducible expression of the liver-enriched inhibitor protein isoform of CCAAT/enhancer binding protein β (Clebpβ (Cebpb)) (ClEBPβ-LIP) in fully differentiated 3T3-L1 adipocytes. Because overexpression of Clebpβ-LIP impairs adipocyte differentiation, the Clebpβ-LIP construct was first integrated in 3T3-L1 preadipocytes but expression was induced only when adipocytes were fully differentiated. Increased C/EBPβ-LIP in mature adipocytes down-regulated Clebpβ target genes including 11β-hydroxysteroid dehydrogenase type 1, phosphoenolpyruvate carboxykinase and fatty acid binding protein 4 but had no effect on asparagine synthetase, demonstrating that transcriptional downregulation by Clebpβ-LIP in 3T3-L1 adipocytes is not a general effect. Importantly, these genes were modulated in a similar manner in adipose tissue of mice with genetically increased Clebpβ-LIP levels. The use of the pSLIK system to conditionally express transgenes in 3T3-L1 cells could be a valuable tool to dissect adipocyte physiology.

Original languageEnglish
Pages (from-to)91-98
Number of pages8
JournalJournal of Molecular Endocrinology
Volume51
Issue number1
DOIs
Publication statusPublished - 2013
Externally publishedYes

Fingerprint Dive into the research topics of 'Stable conditional expression and effect of C/EBPβ-LIP in adipocytes using the pSLIK system'. Together they form a unique fingerprint.

Cite this