Source tracking using microbial community fingerprints: Method comparison with hydrodynamic modelling

D. T. McCarthy, D. Jovanovic, A. Lintern, I. Teakle, M. Barnes, A. Deletic, R. Coleman, G. Rooney, T. Prosser, S. Coutts, M. R. Hipsey, L. C. Bruce, R. Henry

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Urban estuaries around the world are experiencing contamination from diffuse and point sources, which increases risks to public health. To mitigate and manage risks posed by elevated levels of contamination in urban waterways, it is critical to identify the primary water sources of contamination within catchments. Source tracking using microbial community fingerprints is one tool that can be used to identify sources. However, results derived from this approach have not yet been evaluated using independent datasets. As such, the key objectives of this investigation were: (1) to identify the major sources of water responsible for bacterial loadings within an urban estuary using microbial source tracking (MST) using microbial communities; and (2) to evaluate this method using a 3-dimensional hydrodynamic model. The Yarra River estuary, which flows through the city of Melbourne in South-East Australia was the focus of this study. We found that the water sources contributing to the bacterial community in the Yarra River estuary varied temporally depending on the estuary's hydrodynamic conditions. The water source apportionment determined using microbial community MST correlated to those determined using a 3-dimensional hydrodynamic model of the transport and mixing of a tracer in the estuary. While there were some discrepancies between the two methods, this investigation demonstrated that MST using bacterial community fingerprints can identify the primary water sources of microorganisms in an estuarine environment. As such, with further optimization and improvements, microbial community MST has the potential to become a powerful tool that could be practically applied in the mitigation of contaminated aquatic systems.

Original languageEnglish
Pages (from-to)253-265
Number of pages13
JournalWater Research
Volume109
DOIs
Publication statusPublished - 1 Feb 2017

Fingerprint

Estuaries
microbial community
Hydrodynamics
hydrodynamics
estuary
modeling
Contamination
Water
water
Rivers
estuarine environment
Public health
river
Microorganisms
Catchments
point source
comparison
method
public health
mitigation

Cite this

McCarthy, D. T., Jovanovic, D., Lintern, A., Teakle, I., Barnes, M., Deletic, A., ... Henry, R. (2017). Source tracking using microbial community fingerprints: Method comparison with hydrodynamic modelling. Water Research, 109, 253-265. https://doi.org/10.1016/j.watres.2016.11.043
McCarthy, D. T. ; Jovanovic, D. ; Lintern, A. ; Teakle, I. ; Barnes, M. ; Deletic, A. ; Coleman, R. ; Rooney, G. ; Prosser, T. ; Coutts, S. ; Hipsey, M. R. ; Bruce, L. C. ; Henry, R. / Source tracking using microbial community fingerprints : Method comparison with hydrodynamic modelling. In: Water Research. 2017 ; Vol. 109. pp. 253-265.
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McCarthy, DT, Jovanovic, D, Lintern, A, Teakle, I, Barnes, M, Deletic, A, Coleman, R, Rooney, G, Prosser, T, Coutts, S, Hipsey, MR, Bruce, LC & Henry, R 2017, 'Source tracking using microbial community fingerprints: Method comparison with hydrodynamic modelling' Water Research, vol. 109, pp. 253-265. https://doi.org/10.1016/j.watres.2016.11.043

Source tracking using microbial community fingerprints : Method comparison with hydrodynamic modelling. / McCarthy, D. T.; Jovanovic, D.; Lintern, A.; Teakle, I.; Barnes, M.; Deletic, A.; Coleman, R.; Rooney, G.; Prosser, T.; Coutts, S.; Hipsey, M. R.; Bruce, L. C.; Henry, R.

In: Water Research, Vol. 109, 01.02.2017, p. 253-265.

Research output: Contribution to journalArticle

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T2 - Method comparison with hydrodynamic modelling

AU - McCarthy, D. T.

AU - Jovanovic, D.

AU - Lintern, A.

AU - Teakle, I.

AU - Barnes, M.

AU - Deletic, A.

AU - Coleman, R.

AU - Rooney, G.

AU - Prosser, T.

AU - Coutts, S.

AU - Hipsey, M. R.

AU - Bruce, L. C.

AU - Henry, R.

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KW - Community profiling

KW - Faecal source tracking

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