Soil disturbance and water stress interact to influence arbuscular mycorrhizal fungi, rhizosphere bacteria and potential for N and C cycling in an agricultural soil

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Abstract

The objective of this study was to determine how soil disturbance and soil water deficit alter colonisation of roots by naturally occurring arbuscular mycorrhizal (AM) fungi and rhizosphere bacteria. Soil cores were collected at the end of summer from a cropped paddock with a 5-year history of no-tillage in south-western Australia which has a Mediterranean climate. Well-watered and water-stressed treatments were maintained at 70 and 35% field capacity, respectively. AM fungal colonisation was determined using microscopy, rhizosphere bacterial community composition was assessed using barcoded PCR-amplified bacterial 16S rRNA genes, and Phylogenetic Investigation of Communities by Reconstruction of Unobserved States (PICRUSt) analysis of functional gene prediction was used to characterise the rhizosphere functionality for some nitrogen (N) cycling and soil carbon (C) degradation. Mycorrhizal colonisation and plant growth were reduced by disturbance under water stress but not for well-watered soil cores. The rhizosphere bacterial community composition shifted with both soil disturbance and soil moisture, and there was an interaction between them. Disturbance decreased the relative abundance of Proteobacteria and Acidobacteria and increased the relative abundance of Actinobacteria and Firmicutes in water-stressed soil. The predicted abundance of some genes involved in N reactions was negatively influenced by both disturbance and soil moisture, but the predicted abundance of C degrading predicted genes was only marginally affected. This study highlighted how soil disturbance prior to seeding can alter soil biological processes that influence plant growth and that this could be most pronounced when water is limiting.

Original languageEnglish
Pages (from-to)53-66
Number of pages14
JournalBiology and Fertility of Soils
Volume55
Issue number1
Early online date3 Nov 2018
DOIs
Publication statusPublished - Jan 2019

Cite this

@article{0883f590d4194966ba3b4dd4ed908e71,
title = "Soil disturbance and water stress interact to influence arbuscular mycorrhizal fungi, rhizosphere bacteria and potential for N and C cycling in an agricultural soil",
abstract = "The objective of this study was to determine how soil disturbance and soil water deficit alter colonisation of roots by naturally occurring arbuscular mycorrhizal (AM) fungi and rhizosphere bacteria. Soil cores were collected at the end of summer from a cropped paddock with a 5-year history of no-tillage in south-western Australia which has a Mediterranean climate. Well-watered and water-stressed treatments were maintained at 70 and 35{\%} field capacity, respectively. AM fungal colonisation was determined using microscopy, rhizosphere bacterial community composition was assessed using barcoded PCR-amplified bacterial 16S rRNA genes, and Phylogenetic Investigation of Communities by Reconstruction of Unobserved States (PICRUSt) analysis of functional gene prediction was used to characterise the rhizosphere functionality for some nitrogen (N) cycling and soil carbon (C) degradation. Mycorrhizal colonisation and plant growth were reduced by disturbance under water stress but not for well-watered soil cores. The rhizosphere bacterial community composition shifted with both soil disturbance and soil moisture, and there was an interaction between them. Disturbance decreased the relative abundance of Proteobacteria and Acidobacteria and increased the relative abundance of Actinobacteria and Firmicutes in water-stressed soil. The predicted abundance of some genes involved in N reactions was negatively influenced by both disturbance and soil moisture, but the predicted abundance of C degrading predicted genes was only marginally affected. This study highlighted how soil disturbance prior to seeding can alter soil biological processes that influence plant growth and that this could be most pronounced when water is limiting.",
keywords = "Soil microbiology, PICRUSt, Abiotic stress, Next generation sequencing, MICROBIAL COMMUNITY STRUCTURE, EXTERNAL HYPHAE, CARBON, RESPONSES, ROOT, DIVERSITY, TILLAGE, DROUGHT, COLONIZATION, INFECTIVITY",
author = "Mickan, {Bede S.} and Abbott, {Lynette K.} and Solaiman, {Zakaria M.} and Falko Mathes and Siddique, {Kadambot H. M.} and Jenkins, {Sasha N.}",
year = "2019",
month = "1",
doi = "10.1007/s00374-018-1328-z",
language = "English",
volume = "55",
pages = "53--66",
journal = "Biology and Fertility of Soils",
issn = "0178-2762",
publisher = "Springer",
number = "1",

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TY - JOUR

T1 - Soil disturbance and water stress interact to influence arbuscular mycorrhizal fungi, rhizosphere bacteria and potential for N and C cycling in an agricultural soil

AU - Mickan, Bede S.

AU - Abbott, Lynette K.

AU - Solaiman, Zakaria M.

AU - Mathes, Falko

AU - Siddique, Kadambot H. M.

AU - Jenkins, Sasha N.

PY - 2019/1

Y1 - 2019/1

N2 - The objective of this study was to determine how soil disturbance and soil water deficit alter colonisation of roots by naturally occurring arbuscular mycorrhizal (AM) fungi and rhizosphere bacteria. Soil cores were collected at the end of summer from a cropped paddock with a 5-year history of no-tillage in south-western Australia which has a Mediterranean climate. Well-watered and water-stressed treatments were maintained at 70 and 35% field capacity, respectively. AM fungal colonisation was determined using microscopy, rhizosphere bacterial community composition was assessed using barcoded PCR-amplified bacterial 16S rRNA genes, and Phylogenetic Investigation of Communities by Reconstruction of Unobserved States (PICRUSt) analysis of functional gene prediction was used to characterise the rhizosphere functionality for some nitrogen (N) cycling and soil carbon (C) degradation. Mycorrhizal colonisation and plant growth were reduced by disturbance under water stress but not for well-watered soil cores. The rhizosphere bacterial community composition shifted with both soil disturbance and soil moisture, and there was an interaction between them. Disturbance decreased the relative abundance of Proteobacteria and Acidobacteria and increased the relative abundance of Actinobacteria and Firmicutes in water-stressed soil. The predicted abundance of some genes involved in N reactions was negatively influenced by both disturbance and soil moisture, but the predicted abundance of C degrading predicted genes was only marginally affected. This study highlighted how soil disturbance prior to seeding can alter soil biological processes that influence plant growth and that this could be most pronounced when water is limiting.

AB - The objective of this study was to determine how soil disturbance and soil water deficit alter colonisation of roots by naturally occurring arbuscular mycorrhizal (AM) fungi and rhizosphere bacteria. Soil cores were collected at the end of summer from a cropped paddock with a 5-year history of no-tillage in south-western Australia which has a Mediterranean climate. Well-watered and water-stressed treatments were maintained at 70 and 35% field capacity, respectively. AM fungal colonisation was determined using microscopy, rhizosphere bacterial community composition was assessed using barcoded PCR-amplified bacterial 16S rRNA genes, and Phylogenetic Investigation of Communities by Reconstruction of Unobserved States (PICRUSt) analysis of functional gene prediction was used to characterise the rhizosphere functionality for some nitrogen (N) cycling and soil carbon (C) degradation. Mycorrhizal colonisation and plant growth were reduced by disturbance under water stress but not for well-watered soil cores. The rhizosphere bacterial community composition shifted with both soil disturbance and soil moisture, and there was an interaction between them. Disturbance decreased the relative abundance of Proteobacteria and Acidobacteria and increased the relative abundance of Actinobacteria and Firmicutes in water-stressed soil. The predicted abundance of some genes involved in N reactions was negatively influenced by both disturbance and soil moisture, but the predicted abundance of C degrading predicted genes was only marginally affected. This study highlighted how soil disturbance prior to seeding can alter soil biological processes that influence plant growth and that this could be most pronounced when water is limiting.

KW - Soil microbiology

KW - PICRUSt

KW - Abiotic stress

KW - Next generation sequencing

KW - MICROBIAL COMMUNITY STRUCTURE

KW - EXTERNAL HYPHAE

KW - CARBON

KW - RESPONSES

KW - ROOT

KW - DIVERSITY

KW - TILLAGE

KW - DROUGHT

KW - COLONIZATION

KW - INFECTIVITY

U2 - 10.1007/s00374-018-1328-z

DO - 10.1007/s00374-018-1328-z

M3 - Article

VL - 55

SP - 53

EP - 66

JO - Biology and Fertility of Soils

JF - Biology and Fertility of Soils

SN - 0178-2762

IS - 1

ER -