SLIRP stabilizes LRPPRC via an RRM-PPR protein interface

H. Spåhr, A. Rozanska, X. Li, I. Atanassov, R.N. Lightowlers, Z.M.A. Chrzanowska-Lightowlers, Oliver Rackham, N.G. Larsson

Research output: Contribution to journalArticlepeer-review

35 Citations (Scopus)


© 2016 The Author(s) 2016. Published by Oxford University Press on behalf of Nucleic Acids Research.
LRPPRC is a protein that has attracted interest both for its role in post-transcriptional regulation of mitochondrial gene expression and more recently because numerous mutated variants have been characterized as causing severe infantile mitochondrial neurodegeneration. LRPPRC belongs to the pentatricopeptide repeat (PPR) protein family, originally defined by their RNA binding capacity, and forms a complex with SLIRP that harbours an RNA recognition motif (RRM) domain. We show here that LRPPRC displays a broad and strong RNA binding capacity in vitro in contrast to SLIRP that associates only weakly with RNA. The LRPPRC-SLIRP complex comprises a hetero-dimer via interactions by polar amino acids in the single RRM domain of SLIRP and three neighbouring PPR motifs in the second quarter of LRPPRC, which critically contribute to the LRPPRC-SLIRP binding interface to enhance its stability. Unexpectedly, specific amino acids at this interface are located within the PPRs of LRPPRC at positions predicted to interact with RNA and within the RNP1 motif of SLIRP's RRM domain. Our findings thus unexpectedly establish that despite the prediction that these residues in LRPPRC and SLIRP should bind RNA, they are instead used to facilitate protein-protein interactions, enabling the formation of a stable complex between these two proteins.
Original languageEnglish
Pages (from-to)6868-6882
Number of pages15
JournalNucleic Acids Research
Issue number14
Early online date28 Jun 2016
Publication statusPublished - 19 Aug 2016


Dive into the research topics of 'SLIRP stabilizes LRPPRC via an RRM-PPR protein interface'. Together they form a unique fingerprint.

Cite this