Single-tube protocol for the extraction of DNA or RNA from paraffin-embedded tissues using a starch-based adhesive

Gavin R. Turbett, Timothy C. Barnett, Elizabeth K. Dillon, Loryn N. Sellner

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

We describe a method using an inexpenive craft glue to routinely isolate specific areas of tissue as small as 1 mm2 from paraffin sections. The tissue may be digested to release nucleic acid suitable for PCR or reverse transcription PCR. The use of this procedure obviates the requirement for manual microdissection or ultraviolet light irradiation. Tissue remaining on the slide can be stained and analyzed, allowing the precision of the extraction to be determined. The slide can be stored as a permanent record of the material taken for analysis.

Original languageEnglish
Pages (from-to)846-853
Number of pages8
JournalBioTechniques
Volume20
Issue number5
DOIs
Publication statusPublished - May 1996
Externally publishedYes

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