Single-nucleus RNA sequencing reveals novel molecular signatures of healthy and chronically injured liver

Rodrigo Carlessi, Julia Köhn-Gaone, ND Abu Bakar, Elena Denisenko, Matt Jones, Daniel Poppe, George Yeoh, Alistair Forrest, Nina Tirnitz-Parker

Research output: Contribution to journalAbstract/Meeting Abstract

Abstract

Single‐cell transcriptome sequencing (scRNA‐seq) has recently revolutionized the understanding of mammalian liver biology and generated data at unprecedented detail. An obstacle in such studies, however, has been to generate highly viable single‐cell suspensions that represent a true snapshot of the intact liver tissue, without activation of de novo transcriptional stress responses. Past studies implemented diverse enrichment strategies to investigate rare and/or difficult‐to‐dissociate cell types; however, these approaches compromised normal cellular representation. Our aims were to: (a) develop an unbiased sampling approach to profile hepatic cells by single‐nucleus RNA sequencing (snRNA‐seq) of flash‐frozen tissue samples; (b) compare transcriptomics of healthy versus chronically injured liver; and (c) identify novel molecular signatures and potential diagnostic or prognostic biomarkers.
Original languageEnglish
Pages (from-to)25-26
Number of pages2
JournalJournal of Gastroenteology and Hepatology
Volume35
Issue numberS1
DOIs
Publication statusPublished - 11 Nov 2020

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