Projects per year
Abstract
Individual cells in an organism are variable, which strongly impacts cellular processes. Advances in sequencing technologies have enabled single-cell genomic analysis to become widespread, addressing shortcomings of analyses conducted on populations of bulk cells. While the field of single-cell plant genomics is in its infancy, there is great potential to gain insights into cell lineage and functional cell types to help understand complex cellular interactions in plants. In this review, we discuss current approaches for single-cell plant genomic analysis, with a focus on single-cell isolation, DNA amplification, next-generation sequencing, and bioinformatics analysis. We outline the technical challenges of analysing material from a single plant cell, and then examine applications of single-cell genomics and the integration of this approach with genome editing. Finally, we indicate future directions we expect in the rapidly developing field of plant single-cell genomic analysis.
Original language | English |
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Article number | 50 |
Journal | Genes |
Volume | 9 |
Issue number | 1 |
DOIs | |
Publication status | Published - 22 Jan 2018 |
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Dive into the research topics of 'Single-cell genomic analysis in plants'. Together they form a unique fingerprint.Projects
- 2 Finished
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Establishing Novel Breeding Methods for Canola Improvement
Batley, J. (Investigator 01), Edwards, D. (Investigator 02), Pike, D. (Investigator 03), Raman, H. (Investigator 04) & Rae, S. (Investigator 05)
ARC Australian Research Council , Bayer AG, Department of Enterprise, Investment and Trade (New South Wales)
1/02/15 → 31/12/17
Project: Research
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Defining the Brassica Pan-genome and Establishing Methods for Gene Conversion Based Crop Improvement
Batley, J. (Investigator 01), Edwards, D. (Investigator 02) & Laga, B. (Investigator 03)
ARC Australian Research Council , Bayer AG
1/01/14 → 30/09/18
Project: Research